Contributors: Bruno Camargo dos Santos, Vanessa Stramantinoli Rossi, Bruno Goncalves, Fabio Zanuzzo, Juliane Leutz, Percília Cardoso Giaquinto
... In the present paper, we assess whether fish feeding responses and aggressive behavior can be affected by a hook injury (C&R). We assessed this question using an aggressive cichlid fish species, the Nile tilapia (Oreochromis niloticus), which displays pronounced dominance hierarchies and is a territorialist species. Hierarchy and territorialism are dependent of aggressivity and the nutritional state of individuals and thus may be affected by C&R. We exposed fish to three treatments: control (no-handling), chase/capture (C&C) and catch/release (C&R). Fish had their feeding responses (latency to feed and time to eat all feed pellets) evaluated prior to and at 2, 24 and 48 h post-handling, and the aggressive behavior was evaluated prior to and at 2, 6, 24 and 48 h post-handling. The aggressive behavior (latency, number of bites and confront index) was evaluated through the mirror test. We observed that 2 h after the catch-and-release, fish took a longer time to eat all pellets. In general, control fish displayed a consistent increase in aggressive behavior over time, and this pattern was interrupted by C&C, and an even larger response was seen in fish exposed to C&R, which had a consistent reduction in the number of bites post-handling compared to other groups. Although fish showed a tendency to become less aggressive post-C&R handling, catch-and-release did not largely affect their feeding responses or aggressive behavior, demonstrating that Nile tilapia is resistant/resilient to physical handling such as C&C or C&R.
Contributors: Chaitali Misra
... Myotonic dystrophy type 1 (DM1) is a dominantly inherited neuromuscular disease caused by a CTG repeat expansion in 3’-UTR of DMPK gene. DM1 affects multiple tissues, but cardiac dysfunctions are the second leading cause of death. The best characterized pathogenic mechanism of DM1 is toxic gain-of-function of expanded CUG repeat (CUGexp) RNA that accumulates to form ribonuclear foci causing sequestration of MBNL and overexpression of CELF1 family of splicing factors. However, loss of MBNL or gain of CELF1 activity does not explain the cardiac phenotypes observed in DM1. Here we report that steady-state protein levels of RBFOX2, a critical splicing regulator, are drastically upregulated in DM1 heart tissue. This is accompanied by aberrant skipping of a muscle-specific 43bp exon in RBFOX2 transcript, resulting in selective up-regulation of the non-muscle RBFOX2 splice isoform in adult cardiomyocytes. We demonstrate that expression of CUGexp RNA in DM1 affects RBFOX2 in two distinct ways: reduced expression of a subset of microRNAs causes de-repression of RBFOX2 protein production; and CELF1 overexpression promotes skipping of the muscle-specific exon of RBFOX2. Remarkably, tet-inducible overexpression of the non-muscle RBFOX2 isoform, or CRISPR/Cas9 mediated deletion of the muscle-specific RBFOX2 exon in the mouse heart results in prolonged PR and QR intervals, slower conduction velocity and spontaneous cardiac arrhythmias that mirror human DM1 pathology. Integration of RBFOX2-CLIP with RNA-seq data from cardiomyocytes of mice expressing the non-muscle RBFOX2 isoform identified a core network of mRNA splicing defects in genes encoding sodium, potassium, and calcium ion channels that are similarly misspliced in hearts of DM1 patients. These aberrantly spliced ion channels alter their rate of ion diffusion and electrophysiological properties thereby induce cardiac arrhythmias in DM1 heart. Thus, we have uncovered a crucial role for RBFOX2 isoform switching in DM1 cardiac pathogenesis
Contributors: Martin Bitomský, Pavla Mládková, Robin Pakeman, Martin Duchoslav
... Data from: Bitomský M., Mládková P., Pakeman RJ, & Duchoslav M. (accepted pending minor revision 12.11.2020). Clade composition of a plant community indicates its phylogenetic diversity. Ecology and Evolution Data summarises results from the case studies and simulations presented in our paper. In addition, we provide an R script for calculation of proposed phylogenetic diversity metrics (the clade indices). Brief description of each file: 1. Grasslands_DNA_markers_info.xls - Accession numbers of all DNA markers used for phylogeny inference in grasslands 2. Grasslands_DNA_alignment_BEFORE_GBlocks.fasta - DNA alignment matrix before utilisation of the GBlocks tool 3. Grasslands_DNA_alignment_AFTER_GBlocks.fasta - DNA alignment matrix after utilisation of the GBlocks tool 4. Grasslands_BEAST_file.xml - BEAST .xml file submitted to the CIPRES portal (www.phylo.org) 5. Grasslands_tree.txt - Dated MCC tree, grasslands (newick format) 6. Phyto-database_pruned_tree.txt - Pruned dated tree from the super tree of European flora (Durka & Michalski 2012, Ecology), phytosociological database (newick format) 7. Plot_data.slx - plot data of all case studies + species lists 8. Simulation_results.txt - Summary of R2 values (phylogeny-based metric ~ the clade index) for simulated phylogenies and community matrices (manipulated: phylogenetic scale, species pool size and species richness range) 9. Bitomsky2020EE_R_script_indices.R - An R script for computation of the clade indices (with notes and examples)
Deriving ecological site metrics for mapping rangeland resource units in an extensive East African pastoral landscape
Contributors: Mohamed Shibia, Achim Röder, Francesco Fava, Marion Stellmes, Joachim Hill
... GIS data and data codes supporting satellite data interpretation scheme for categorizing and mapping different rangeland resource units in an extensive African landscape where field observation is irregular and sparse.
Contributors: Mischa Haas, Petra Boltshauser-Kaltenrieder, Nathalie Dubois, Nemiah Ladd, Michael Strasser, Timothy Ian Eglinton, Caroline Welte
... Data tables for Haas et al. "Land-use evolution in the catchment of Lake Murten, Switzerland"
Contributors: divya mohan, geetha mary
... Scheduling procedures implemented in the wireless networks include a variety of workflows such as resource allocation, channel gain improvement and reduction in packet arrival delay. Among these techniques, the Long Term Evolution (LTE) scheduling is highly preferable due to its high speed of communication and less bandwidth consumption. The LTE assigns resources to the workflows based on the time and frequency domains. Normally, the information gathering prior to scheduling increases the processing time due to the verification of each attribute of the user. To solve this issue, parallel processing through data mining is employed in the recent research studies. The label can be assigned to the user attributes which contributes highly towards scheduling of time slots effectively. The label assignment and parallel processing through data mining concept reduces the delay and increases the throughput. Besides, the extraction of matched data from the library and the prediction of available channels with fewer dimensions are the major issues in the LTE scheduling. Traditionally, numerous approaches are developed for LTE scheduling by solving the problems related to data missing values and classification. Based on this, a powerful setup is created for data mining on handoff procedures in LTE communication. The data initially can be obtained from the network service providers, based on our process the data is taken for analysis. This dataset provides you the information on user handoff related data attributes for analysis.
Data for "Reassessing enzyme kinetics: Considering protease-assubstrate interactions in proteolytic networks"
Contributors: Manu Platt
... Data to accompany the PNAS paper of the same title with authors Meghan Ferrall-Fairbanks, Chris Kieslich, and Manu Platt
Data for: Paleoenvironmental change on coastal SE Zagros during the last two millennia using paleoreconstruction of Khuran Estuary
Contributors: Mohammad Ali Hamzeh, Sedigheh Farahi Ghasr-Aboonasr
... Data are about physical properties of the sediment core, distribution of foraminifers, ages and GIS-based shapefiles.
Questionnaire data of innovation ambidexterity, absorptive capacity and sustainable competitive advantage
Contributors: Astadi Pangarso
... This research data were collected through 530 questionnaire. The research variable are: X: absorptive capacity Y1: innovation ambidexterity Y2: sustainable competitive advantage
Dataset for Validation for Development of Authentic-based Competency Assessment Rubric for Secondary School Vocational Student: Malaysian Context
Contributors: Nithia K, Farrah Dina Yusop, Yan Piaw Chua , akhmad habibi
... This data set consists of list of experts' information that are involved in validating research's assessment rubric's instruments. The rubric was developed to assess secondary school vocational students' competency skill in authentic-based learning strategy within Malaysian context. The instruments was developed based on four constructs ( Teamwork, Skills, Knowledge and Presentation). It has 20 criterions and it is used to assess students' competency based on authentic-based tasks. The rubric was given to 12 experts in Technology, Vocational and Education Technology (TVET) to evaluated the rubric. Six of them have responded and their responses was used to calculate Content Validity Ratio (CVR) and Content Validity Index (CVI) based on Lawshe Method. Sufficient values of CVR and CVI for each and all of the items determine the validity and reliability of the rubric.