Data for: The nuclear transcription factor FoxG1 affects the sensitivity of mimetic aging hair cells to inflammation by regulating autophagy pathways
Contributors: Wei-Jia Kong, Hai-ying Sun, YU SUN, Zuhong He, Xiao-xiang Xu, Sen Chen, Dan Li, Xia Wu, Ming Li, renjie chai
... Inflammation is a self-defense response to protect individuals from infection and tissue damage, but excessive or persistent inflammation can have adverse effects on cell survival. Many individuals become especially susceptible to chronic- inflammation-induced sensorineural hearing loss as they age, but the intrinsic molecular mechanism behind aging individuals' increased risk of hearing loss remains unclear. FoxG1 (forkhead box transcription factor G1) is a key transcription factor that plays important roles in hair cell survival through the regulation of mitochondrial function, but how the function of FoxG1 changes during aging and under inflammatory conditions is unknown. In this study, we first found that FoxG1 expression and autophagy both increased gradually in the low concentration lipopolysaccharide (LPS)-induced inflammation model, while after high concentration of LPS treatment both FoxG1 expression and autophagy levels decreased as the concentration of LPS increased. We then used siRNA to downregulate Foxg1 expression in hair cell-like OC-1 cells and found that cell death and apoptosis were significantly increased after LPS injury. Furthermore, we used D-galactose (D-gal) to create an aging model with hair cell-like OC-1 cells and cochlear explant cultures in vitro and found that the expression of Foxg1 and the level of autophagy were both decreased after D-gal and LPS co-treatment. Lastly, we knocked down the expression of Foxg1 under aged inflammation conditions and found increased numbers of dead and apoptotic cells. Together these results suggest that FoxG1 affects the sensitivity of mimetic aging hair cells to inflammation by regulating autophagy pathways.
Contributors: Dmitrii Maltsev
... The data includes en face optical coherence tomography angiography images from mild hypertensive patients and healthy individuals. Each image constructed with a 9-µm slab at the lower border of the outer plexiform layer and aims to demonstrates the presence or absence of chronic/resolved paracentral acute middle maculopathy (PAMM) lesions appearing as small dark areas. In the case where at least one PAMM lesion present corresponding cross-sectional scan shows structural appearance of that.
Reshaping commensal gut microbiota in early life with amoxicillin presents with lower blood pressure
Contributors: Saroj Chakraborty
... Pediatric hypertension is recognized as an emerging global health concern. While new guidelines are developed for facilitating clinical management, the reasons for the prevalence of hypertension in children remain unknown. Genetics and environmental factors do not fully account for the growing incidence of pediatric hypertension. Because stable bacterial flora in early life are linked with health outcomes later in life, we hypothesized that reshaping of gut microbiota in early developmental stages of life affects blood pressure (BP) of pediatric subjects. To test this hypothesis, we administered amoxicillin, the most commonly prescribed pediatric antibiotic, to alter gut microbiota of young, genetically hypertensive rats (study 1) and dams during gestation and lactation to reshape microbiota of offspring (study 2). Reshaping of microbiota, with reductions in Firmicutes/Bacteriodetes ratio observed in Amoxicillin treated young rats and in dams. Amoxicillin treated rats also had lower blood pressure compared to the untreated rats. In the young rats treated with amoxicillin, the lowering effect on blood pressure persisted even after the antibiotics were discontinued. Similarly, the offspring from the dams treated with amoxicillin also showed lower systolic blood pressure compared to the control rats. Remarkably, in all cases, a decrease in BP was associated with lowering of Veillonellaceae, which are succinate-producing bacteria. Elevated plasma succinate is reported in hypertension. Accordingly, serum succinate was measured and found lower in animals treated with amoxicillin. Our results demonstrate a direct correlation between succinate-producing gut microbiota and early development of hypertension, and indicate that reshaping gut microbiota, especially by depleting succinate-producing microbiota early in life may have long-term benefits for hypertension-prone individuals.
Contributors: Syed Saiden Abbas, Tjeerd M. H. Dijkstra
... This data set has been used in "Detection and stage classification of Plasmodium falciparum from images of Giemsa stained thin blood films using random forest classifier". It was originally collected for the paper https://doi.org/10.1073/pnas.0811829106. The data set contains 883 Giemsa stained images from 17 malaria infected patients. We provide (1) the original Giemsa stained images in PNG format, (2) ground truth images in PNG format and (3) life-stage labels for each parasite with their location in Excel format.
Contributors: Sangarani Sharungbam, Pranav Pandya
... Manipur, a land of the rising sun, is endowed with rich biodiversity and abundant natural resources. Despite inaccessibility, marginality and heterogeneity, the state has made good progress in agriculture and allied sectors. The presence of Mountains causes a barrier effect over the entire region which leads to a protective effect on the flora and fauna. This protective effect has been studied in this research.
Contributors: Matthias Sinnesael, Sietske J. Batenburg, Christian Zeeden, Jiri Laurin, Damien Pas, Linda Hinnov, Johannes Monkenbusch, Maximilian Vahlenkamp, Mingsong Li, Sébastien Wouters
... Supplementary materials for Submission: Sinnesael et al., Earth-Science Reviews "The Cyclostratigraphy Intercomparison Project (CIP): consistency, merits and pitfalls ".
Molecular Identification and Isolation of Antimicrobial, Phytochemical, Antioxidant Properties of Different Solvent Extracts from Wild Mushrooms
Contributors: MANJUSHA PGS
... The main objective of the present research work was to identify the unknown wild mushrooms based on morphological characteristics and DNA based sequencing by using universal primers ITS 1 and ITS 4 which were collected from Andhra Pradesh and Telangana. To test the potential of various extracts of mushrooms by using three different solvents (Methanol, Hot Water and Hexane). To elucidate antimicrobial properties, qualitative and quantitative estimation of phytochemical properties and antioxidant activity was determined by DPPH free radical scavenging activity. Then, IC50 values were determined. The four isolates were identified on the basis of morphological and molecular characterization was identified as Trametes versicolor, Daedaleopsis confragosa, Schizophyllum communae and Tyromyces chioneus. Three different solvents had been shown good antimicrobial activity against all pathogenic microorganisms. The hot water extract of T. versicolor exhibited more amount of phenol content (11.13±0.081) and ascorbic acid content (9.62±0.086). Methanol extract of S. communae showed high amount of flavonoids content (12.58±0.138). Methanol extract of T. chioneus exhibited more amount of β-carotene (3.54±0.010) and hot water extract of D. confragosa exhibited more amount of lycopene content (2.29±0.012). The DPPH free radical scavenging activity and IC50 values was much more exhibited by methanol extracts then followed by hot water extracts and hexane extracts of wild mushrooms they all have exhibited stronger antioxidant activity between (40-50 µg/ml). This comprehensive analysis demonstrates that these mushrooms having a viable source of natural antioxidants that can utilized for functional foods, nutraceutical’s applications with beneficial biological activities and implementation of new drug discovery.
A histopathological image repository of normal epithelium of Oral Cavity and Oral Squamous Cell Carcinoma
Contributors: Tabassum Yesmin Rahman
... Total 1224 images are there in this repository. Images are divided into two sets in two different resolutions. First set composed of 89 histopathological images with the normal epithelium of the oral cavity and 439 images of Oral Squamous Cell Carcinoma (OSCC) in 100x magnification. The second set consists of 201 images with the normal epithelium of the oral cavity and 495 histopathological images of OSCC in 400x magnifications. The images were captured using a Leica ICC50 HD microscope from H&E stained tissue slides collected, prepared and catalogued by medical experts from 230 patients.
Contributors: Camilo Sanchez-Ferreira, Carlos Llanos, Mylene Farias, Leandro Coelho, HELON VICENTE HULTMANN AYALA, Jose Mauricio Motta
... containing 135 underwater images taken at different levels of turbidity, different distances between camera and object and using different colors of the objects. The dataset also contains 45 reference images (captured with no water) and the corresponding subjective quality scores for degraded images. The subjective quality scores were obtained by performing a subjective quality assessment experiment based on Single Stimulus technique with voluntary participants that rated the quality of the test images.
Photodynamic therapy for cutaneous squamous cell carcinoma in situ: impact of anatomic location, tumor diameter and incubation time on effectiveness
Contributors: Sean Christensen, Nour Kibbi, David Leffell, Yuemei Zhang
... Background Photodynamic therapy (PDT) has been reported as a treatment for cutaneous squamous cell carcinoma in situ (SCCis) but there is limited data on the effectiveness of PDT with aminolevulinic acid (ALA-PDT). Objective To review the outcomes of SCCis treated with ALA-PDT and examine factors associated with response. Methods Retrospective review identified 58 patients with 68 primary SCCis lesions treated with ALA-PDT and blue light illumination. Patient demographics, lesion features, treatment details, clinical response, and subsequent recurrence were extracted from medical record review. Results Upon completion of PDT, the initial complete response rate was 77.9% and was not associated with the number of PDT treatments. On multivariate analysis, the factors associated with response were location on the face, tumor diameter <2 centimeters, and longer ALA incubation time. Lesions treated with a maximum incubation time of <3 hours had a 53.3% response compared with 84.9% for longer incubation. Subsequent recurrence of SCCis was noted in 7 of 53 cases (13.2%) at a median time of 11.7 months. Limitations This was a retrospective study performed at a single institution without systematic follow-up. Conclusions ALA-PDT may be an effective treatment for selected cases of SCCis. Effectiveness is impacted by anatomic location, tumor diameter, and ALA incubation time. This Dataset is Supplementary Data for the manuscript, "Photodynamic therapy for cutaneous squamous cell carcinoma in situ: impact of anatomic location, tumor diameter and incubation time on effectiveness." Authors: Nour Kibbi, MD, Yuemei Zhang, MD, MBA, David J. Leffell, MD, and Sean R. Christensen, MD, PhD Accepted for publication by Journal of the American Academy of Dermatology 2019 (JAAD-D-19-01312R1). Supplementary Figure 1. Response of SCCis according to number of PDT treatments. A, Initial complete response for all lesions and according to number of PDT treatments. B, Durable complete response at final follow-up for all lesions and according to number of PDT treatments. Supplementary Figure 2. Occlusion during PDT incubation period was significantly associated with incubation time. P value was calculated using the Spearman correlation. Supplementary Table. Factors associated with recurrence of SCCis after initial complete response.