Data/Software for "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse"
Contributors: Connon I. Thomas, Christian Keine, Satoko Okayama, Rachel Satterfield, Morgan Musgrove, Debbie Guerrero-Given, Naomi Kamasawa, Samuel M. Young, Jr.
... Contains data and software from the publication: "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse" published in the The Journal of Neuroscience (https://doi.org/10.1523/JNEUROSCI.0363-19.2019). The preprint to this data set has been published on bioRxiv (https://doi.org/10.1101/689653). In this study, we created a helper-dependent adenoviral vector (HdAd) to co-express cytoplasmic EGFP and a genetically encoded peroxidase marker (mito-APEX2) at the calyx of Held, an excellent model for deciphering regulatory mechanisms of presynaptic function. ABSTRACT: The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support the high action-potential firing rates required for auditory information encoding. In addition, calyx terminals are morphologically diverse which impacts vesicle release properties and synaptic plasticity. Mitochondria influence synaptic plasticity through calcium buffering and are crucial for providing the energy required for synaptic transmission. Therefore, it has been postulated that mitochondrial levels increase during development and contribute to the morphological-functional diversity in the mature calyx. However, the developmental profile of mitochondrial volumes and subsynaptic distribution at the calyx of Held remains unclear. To provide insight on this, we developed a helper-dependent adenoviral vector (HdAd) that expresses the genetically encoded peroxidase marker for mitochondria, mito-APEX2, at the mouse calyx of Held. We developed protocols to detect labeled mitochondria for use with serial block face scanning electron microscopy to carry out semi-automated segmentation of mitochondria, high-throughput whole terminal reconstruction and presynaptic ultrastructure in mice of either sex. Subsequently, we measured mitochondrial volumes and subsynaptic distributions at the immature postnatal day 7 (P7) and the mature (P21) calyx. We found an increase of mitochondria volumes in terminals and axons from P7 to P21 but did not observe differences between stalk and swelling subcompartments in the mature calyx. Based on these findings, we propose that mitochondrial volumes and synaptic localization developmentally increase to support high firing rates required in the initial stages of auditory information processing. Data are sorted by the figures they appear in. Media (movies and 3D models) and custom-written software are located in separate folders.
Contributors: Nikhil Kaza
... This curated dataset is derived from public sources. The aim was to link urban form measurements to energy consumption as proxied by sales in gas stations in the United States. The geographic resolution is a county.
Contributors: Alessandro Olper, Marzio Galeotti, Chiara Falco
... The files enclosed are related to the dataset (STATA format), and variables description, of the article: "Climate Change and Migration: Is Agriculture the Main Channel? ( Falco, C., Galeotti M. and Olper, A) Submitted to Global Environmental Change.
The relationship between regular sinusoidal waves in the tissue at lung-related acupuncture points and the respiration pacesetter mechanism
Contributors: Fletcher Kovich
... This data compliments a paper entitled: The relationship between regular sinusoidal waves in the tissue at lung-related acupuncture points and the respiration pacesetter mechanism. The paper fully describes and analyses the data. Supplemental to that paper, the following document analyses aspects of the “pace signals” to rule out the possibility that the pace signals are artefact. http://www.curiouspages.com/research/01.pdf At the time of writing, the main paper is still in production. Please see the links at the end of this dataset, which will be updated once the paper is published. A preprint draft of the main paper is available at https://www.preprints.org/manuscript/201910.0201/v2
Confronting the Wall of Patriarchy: Does participatory intrahousehold Decision Making empower Women in agricultural Households?
Contributors: Els Lecoutere, eva Wuyts
... This study investigates the impact of introducing participatory intrahousehold decision-making on the empowerment of women in agricultural households in sub-Saharan Africa, particularly in Ugandan coffee farming households. Participatory intrahousehold decision-making is expected to empower women through increasing their voice and reducing collective action problems, which otherwise compromise efficiency and equity of the household farm. With a mixed methods approach this study captures the impact on multiple dimensions of empowerment, including women’s perceptions of the process, meaning and value. Our study qualitatively finds that involvement in strategic household decisions and cash crops is of key importance to women but hard to achieve. Decision-making power over day-to-day household affairs is assigned to them by default, but carries less meaning. Women portrayed three possible pathways towards empowerment in their household: “Breaking through the wall of patriarchy” – the preferred pathway but conditional on being married to a cooperative husband - “Circumventing” it, or having “No choice but to take full responsibility” in case of husbands who are ill or absent. On the basis of a randomized encouragement of couples to participate in an intervention introducing participatory intrahousehold decision-making, we quantitatively demonstrated the catalyzing effects on different domains of women’s empowerment, including involvement in strategic household decisions, women’s control over household income, personal income and assets. Women’s decision-making power over cash crop production, another valued strategic domain, increased to some extent. These impacts support women in following a pathway to empowerment by “Breaking through the wall of patriarchy”, but are also valuable for women for whom that pathway is out of reach. Alleviating power imbalances and collective action problems through participatory intrahousehold decision-making has the potential to empower women in domains they value and should be combined with effective ways to accomplish women’s wish to gain economic power to actively contribute to their household’s development.
Schistosoma mansoni infection is associated with quantitative and qualitative modifications of the mammalian intestinal microbiota
Contributors: Timothy Jenkins, Laura Peachey, Nadim Ajami, Andrew MacDonald, Michael Hsieh, Paul Brindley, Cinzia Cantacessi, Gabriel Rinaldi
... Raw 16S rRNA sequencing data from luminal contents from sections of the small (SI) and large intestines (LI) of 10 Swiss-Webster female mice (S+) that were infected with 200 S. mansoni cercariae and 10 age- and gender-matched mice that remained uninfected and were included as controls (S-). For each S+ and S- group, five mice were euthanised 28 days after infection (D28 p.i., i.e. before egg laying by sexually mature S. mansoni females had commenced). The remaining mice (n = 5 for each S+ and S-) were euthanised at day 50 p.i. (D50 p.i., corresponding with ongoing egg laying by sexually mature S. mansoni females). The experiment was carried out in duplicate and samples from both experiments (i.e. Exp. 1 & Exp. 2) pooled for further analysis. The data set includes four types of files: 1. Raw joined read files - 1 per sample (demultiplexed): e.g. Exp1_SI_S-_D50_1.fasta 2. Joined, quality filtered file containing reads from all samples and ready for OTU picking. The file was generated using Qiime: seqs.fna 3. Metadata file containing the relevant contextual information for each sample: Schisto_microbiome_Metadata.csv 4. OTU table created using Qiime including all of the taxonomic information: otu_table_mc2_w_tax_no_pynast_failures.biom
Contributors: Eniko Magyari
... This dataset contains the results of multi proxy analyses done on the 20 to 9 kyr cal BP section of a lake sediment core taken from Kokad Mire, E Hungary (47°24’10” N, 21°55’43” E; 112 m a.s.l.). Sediment description, pollen, microcharcoal and major element analyis results are included in the dataset. In addition, input files for quantitative climate reconstruction and output files for biomisation and summer (June-July-August) mean temperature reconstruction are provided with relevant R scripts. The aim of the study was to reconstruct vegetation change, soil development and provide quantitative climate reconstruction for the last glacial termination period for E Hungary and study the early expansion of temperate broadleaved trees (mainly Ulmus and Corylus) in the lowland of E Hungary. Our results suggest that warming was most intensive after Heinrich event 1, around 16,200 cal yr BP, the late glacial climate fluctuation is weakly expressed, the Younger Dryas (GS-1) do not show significant decrease in summer mean temperatures, and hazel shrubs (Corylus) expand in the area at that time confirming that this contintal area was wekly affected by this climate perturbation. The study was published in Quaternary Science Reviews. The paper is available at https://www.sciencedirect.com/science/article/pii/S0277379119305657?dgcid=author or https://doi.org/10.1016/j.quascirev.2019.105980
Contributors: Riccardo Priore
... The dataset includes several Excel sheets, each listing the records obtained from Patstat online database (https://data.epo.org/expert-services/index.html) by means of SQL queries run in Patstat online (Autumn 2017 ed.). Such investigation is aimed at retrieving patent documents data including in particular: • the individual patent application identification code (appln_id attribute in the table), • the application authorities (i.e. the patent offices to whom the patent applications are filed), • the earliest filing year, • the docdb_family_id code univocally identifying a single patent family (a.k.a. DOCDB family), • the patent applications titles and abstracts, • the classification codes (IPC and CPC, respectively) assigned to each patent application, • the patent publication authority, • the patent publication number, • the patent publication kind, • the denomination of each assignee/applicant (psn_name) and • the type of applicant (psn_sector allowing for distinguishing companies, universities, individuals etc. among applicants/assignees). PDF files (named as the corresponding Excel) may be present. Each provides lists of the documents downloadable from PATSTAT online. The number of documents corresponds to the amount of non-duplicated appln_id values (eg. 1125 for PHASE A in the pdf, as arguable from the PHASE A Excel file). URLs are provided for each record to link to Espacenet (https://worldwide.espacenet.com/) • PHASE A TABLE concern a broad search regarding glass recycling. • PHASE B concerns a more specific search, based on keywords AND IPC/CPC codes, regarding recycling of laminated glass by means of mechanical comminution. Data rather based on keywords are argued from PHASE B_query A. Records selected by means of cpc code Y02W30/521 are included in PHASE B Query B TABLE. • PHASE C Query C1 TABLE concerns recycling of glass with poly-vinyl-butyral interlayer (PVB) • PHASE C Query C2’ TABLE concerns recycling of glass with ethyl-vinyl-acetate interlayer (EVA) • PHASE C Query C2’’ TABLE concerns recycling of glass with (thermoplastic) poly-urethane interlayer (TPU). One file with SQL scripts not detailed in other publications is included (PATSTAT SCRIPTS). The file contains two scripts to be run with Patstat: the former is aimed at ranking the patent families depending on the co-occurrence of IPC classification codes, while the latter is aimed at ranking the granted patents depending on the number of annual fee payments and the date of patent validity expiration. SQL scripts are included in a manuscript submitted to Data in Brief journal, accompanying the manuscript submitted to World Patent Information for review (WPI_2018_95 _R1).
Contributors: John Ayers
... Samples of rice paddy soil, tidal channel sediment, and water were collected from five different sites in SW Bangladesh in May and November of 2016 and chemically analyzed to characterize element mobilities and the geochemical factors controlling them. Soil and sediment samples were found to have compositions similar to each other and to average upper continental crust. Disequilibrium partitioning of elements between solids and water or deionized water extracts was observed, although apparent partition coefficients mostly show the expected order of element mobilities. Solid compositions do not change seasonally while surface water compositions do, and little spatial variation was observed.
Data for Gift-giving, Love Styles, and Sacrifice: An Analysis Among Romantic Partners published by RAC-Revista de Administração Contemporânea
Contributors: Jairo de Pontes Gomes, Marianny Brito, Salomão Farias, Vicente Melo
... This paper aimed to understand the gift-giving from the love types experienced in romantic relationships, investigating the frequency, the reasons for the act of giving gifts among couples, and the sacrificial perception. Through a quantitative and descriptive research, we conducted a survey with 257 people. The results revealed that the love characterized by eroticism and passion (Eros) is the predominant style among couples, and it is also, among the primary typologies, that guides individuals to gift more frequently through the social motive. In addition, among the secondary love styles, the altruist (Agape) tends to be what most often induces gift-giving, generating a sacrificial perception of the offerer. Further, it was seen that the sacrifice can be perceived in the gift-giving in other forms of love besides the Agape style. It is concluded that the gift-giving can vary according to the love styles experienced by people and that the perception of sacrifice in this act is related to these typologies of love. From the research results, we encourage the development of reflections and investigations focused on the consequences of love forms on consumer behavior, highlighting Lee's theory (1988) as capable of providing new interpretations about consumer relations.