Data/Software for "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse"
Contributors: Connon I. Thomas, Christian Keine, Satoko Okayama, Rachel Satterfield, Morgan Musgrove, Debbie Guerrero-Given, Naomi Kamasawa, Samuel M. Young, Jr.
... Contains data and software from the publication: "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse" published in the The Journal of Neuroscience (https://doi.org/10.1523/JNEUROSCI.0363-19.2019). The preprint to this data set has been published on bioRxiv (https://doi.org/10.1101/689653). In this study, we created a helper-dependent adenoviral vector (HdAd) to co-express cytoplasmic EGFP and a genetically encoded peroxidase marker (mito-APEX2) at the calyx of Held, an excellent model for deciphering regulatory mechanisms of presynaptic function. ABSTRACT: The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support the high action-potential firing rates required for auditory information encoding. In addition, calyx terminals are morphologically diverse which impacts vesicle release properties and synaptic plasticity. Mitochondria influence synaptic plasticity through calcium buffering and are crucial for providing the energy required for synaptic transmission. Therefore, it has been postulated that mitochondrial levels increase during development and contribute to the morphological-functional diversity in the mature calyx. However, the developmental profile of mitochondrial volumes and subsynaptic distribution at the calyx of Held remains unclear. To provide insight on this, we developed a helper-dependent adenoviral vector (HdAd) that expresses the genetically encoded peroxidase marker for mitochondria, mito-APEX2, at the mouse calyx of Held. We developed protocols to detect labeled mitochondria for use with serial block face scanning electron microscopy to carry out semi-automated segmentation of mitochondria, high-throughput whole terminal reconstruction and presynaptic ultrastructure in mice of either sex. Subsequently, we measured mitochondrial volumes and subsynaptic distributions at the immature postnatal day 7 (P7) and the mature (P21) calyx. We found an increase of mitochondria volumes in terminals and axons from P7 to P21 but did not observe differences between stalk and swelling subcompartments in the mature calyx. Based on these findings, we propose that mitochondrial volumes and synaptic localization developmentally increase to support high firing rates required in the initial stages of auditory information processing. Data are sorted by the figures they appear in. Media (movies and 3D models) and custom-written software are located in separate folders.
Contributors: Aniol Maria
... Designing the Archive was an international archives conference presented by the Australian Society of Archivists (ASA), Archives and Records Association of New Zealand Te Huinga Mahara (ARANZ), the International Council on Archives (ICA) and the Pacific Regional Branch International Council on Archives (PARBICA). It was celebrated in Adelaide, South Australia, from 21 to 25 October 2019. Conference ICA Adelaide 2019 | International Council on Archives https://www.ica.org/en/conference-ica-adelaide-2019 Associació Arxivers a Twitter: "Comença la setmana del Congrés Internacional d'Arxius de la @ICArchiv, us recomanem que en feu seguiment per xarxes: #DTAAdelaide2019 #DesigningTheArchive i @DTAAdelaide2019" https://twitter.com/AAC_GD/status/1186167807739871232 Designing The Archive / Concevoir les archives (@DTAAdelaide2019) / Twitter https://twitter.com/DTAAdelaide2019 Hashtags: #DTAAdelaide2019 #DesigningTheArchive @DTAAdelaide2019 Dates: 2019.10.20-2019.10.26 Number of Tweets: 7,046 Size (Hydrated): 42.3 megabytes
Contributors: Oscar Pupo Roncallo, Javier Campillo, Derek Ingham, Kevin Hughes, Mohammed Pourkashanian
... Data produced for the analysis and development of a high temporal resolution model for the Colombian energy system. The information presented here was collected from international agencies and domestic organisations (SIEL, XM, IEA, UPME, ACOLGEN, IDEAM) and finally filtered and processed. It includes electricity hourly demand data from 2006-2016, natural water inflows time series, renewable energy sources (RES) production distributions and energy exports and imports.
Contributors: Nikolay Bazenkov, Marina Sandomirskaia, Maria Kuznetsova
... The data are the prices collected from market.yandex.ru, the largest Russian online marketplace. The sample includes 30 the most popular household applicances divided into 5 categories: warm ovens, fridges, cooktops, cooker hoods, washing machines and dishwashers. The period is from July 15 to October 12 at 2015. The prices were sampled 4 times per day at 1-00, 7-00, 13-00 and 19-00. The data was collected using the internal API provided by Yandex company. The shops are anonimized due to the Yandex corporate policy. The primary aim of the research was to study price dispersion previosly reported for major online marketplaces such as Amazon.com in US. Folders and files: prices/ - contain 30 files where the filenames are 'product_id.csv' products.csv - contains identifiers, categories and the names of the products The price files have the following structure. The first column stores the timestamps and the other columns store the prices posted by each shop for the given product at the given time. All files have equal number of columns while some columns consist exclusively of NaN values since not every shop offered every product in the sample. timestamp; shop_1; shop_2; ... ; shop_N 4/07/15 01:00; 16860.00; NaN; ...
Online Supplementary Material for: Clements JC, Comeau LA. Behavioural defenses of shellfish prey under ocean acidification. Journal of Shellfish Research
Contributors: Jeff Clements
... Annotated R-script, all raw data files, and a complete dataset accompanying the following article: Clements JC, Comeau LA. 2018. Behavioural defenses of shellfish prey under ocean acidification. Journal of Shellfish Research. (Under Review). All results and methods can be obtained from the manuscript itself.
Data for: Network Pharmacology-based Identification of the Key Mechanism of Qinghuo Rougan Formula Acting on Uveitis.
Contributors: hongsheng bi, Xiaofeng Xie, xueyan zhang, Diana Jing, Sun zhuolun
... We identified 259 major candidate targets of QHRGF and successfully constructed a ‘QHRGF-compound-target-uveitis’ network. Gene enrichment analysis revealed that targets in the cell cycle, autoimmune disease and related signalling pathways were relevant.
Contributors: Pablo Caño
... Raw data files of the main measurements shown in the main manuscript.
Codes and data for "Effects of atmospheric profiles on the perceived loudness of low-boom supersonic aircraft"
Contributors: Pedro Camara Leal
... The effects of atmospheric variability on the acoustic performance of supersonic aircraft are evaluated. Findings show that an economically feasible aircraft (e.g., acceptable fuel consumption) can be quiet for every atmospheric profile and elevation. The geographic distribution of the U.S. population was also considered in measuring the affected population. Furthermore, no noticeable seasonal effects were observed in the evaluated cases.
Processed Data for: Top-Down Mass Spectrometry of Histone Modifications in Sorghum Reveals Potential Epigenetic Markers for Drought Acclimation
Contributors: Mowei Zhou, Neha Malhan, Amirhossein Ahkami, Kristin Engbrecht, Gabriel Myers, Jeffery Dahlberg, Joy Hollingsworth, Julie Sievert, Robert Hutmacher, Mary Madera
... This study explores the changes in histone modifications of Sorghum bicolor (L.) Moench through developmental stages and in response to drought stress in two genotypes. We analyzed the leaves of 48 plants using top-down mass spectrometry and identified 26 unique histone proteins and 677 unique histone proteoforms. This is the collection of processed data and scripts used for data processing.
Data for: Temporal and Spatial Evolution of Instrumented Seismicity in the Trans-Mexican Volcanic Belt.
Contributors: F. Ramon Zuniga, Quetzalcoatl Rodriguez Perez, Pierre Lacan, Víctor H. Márquez-Ramírez
... Catalog of events within and in the vicinity of the Trans-mexican Volcanic Belt region, employed in the analysis.