Contributors: Frazier, J.
... The Aldabran giant tortoise is one of the few testudines that feeds in dense herds, and in so doing the animals frequently exhibit a conspicuous orientation away from the sun. This orientation is most marked when ambient temperature is high, and it breaks down when the tortoises are shielded from direct sunlight. It was previously hypothesized that negative heliotaxis is related to thermoregulation-to reduce heat inflow to the exposed anterior appendages and instead expose the protected posterior of the shell. There has been no test of the thermoregulatory function, and pilot experiments indicate that orientation away form the sun occurs to reduce direct glare into the tortoise's eyes. The glare of the sun seems to interfere with accurately oriented biting movements and reduce feeding efficiency. Speed and intensity of reaction to solar glare is inversely related to the animal's body size. The possibility of a thermoregulatory component to the away-from-the-sun orientation cannot be discounted, but it must be investigated directly.
Contributors: Nagashima, Jennifer B., Wildt, David E., Travis, Alexander J., Songsasen, Nucharin
... Understanding regulators of folliculogenesis remains limited in the domestic dog (Canis familiaris), which challenges our ability to develop in vitro follicle culture systems for canid genome rescue efforts. Here, we investigated the influence of activin on dog follicle development and survival, oocyte quality, and FSH receptor expression in culture. Preantral (150 - 330-550 mu m) stage follicles were encapsulated in a fibrin-alginate hydrogel with 0, 100, or 200 ng/ml rhActivin plus 0, 0.1, 1, or 10 mu g/ml FSH for 12 or 21 d of in vitro culture. All follicle groups increased in diameter (P 330-550 mu m) stage follicles were encapsulated in a fibrin-alginate hydrogel with 0, 100, or 200 ng/ml rhActivin plus 0, 0.1, 1, or 10 mu g/ml FSH for 12 or 21 d of in vitro culture. All follicle groups increased in diameter (P 630 mu m) in early antral and antral cohorts. This complementary effect was not linked to changes in FSHR mRNA expression (P> 0.05). Although not influencing (P> 0.05) follicle survival or transzonal projection (TZP) density in shorter term 12 d culture, activin in the presence of 1 ng/ml FSH maintained TZP density from the 12-21 d interval. Activin also increased oocyte diameter and improved nuclear integrity compared to un-supplemented controls. These results indicate that activin acts synergistically with FSH to promote growth and antral cavity expansion of the dog follicle in vitro, information useful to formulating an effective culture microenvironment for this species. (C) 2019 Elsevier Inc. All rights reserved.
Contributors: Daemmrich, Arthur
... This article reviews the history of drug manufacturing and changes in the compounding of drugs by pharmacies in the United States and outlines opportunities for new research into the making of medicines. The pharmaceutical industry has long been on the vanguard of globalization, and drug companies restructured their international manufacturing footprints frequently in the 19th and 20th centuries. Firms in the prescription drug sector were among the first to market products internationally, to build manufacturing plants around the world, and to integrate research from laboratories across multiple time zones. Yet, issues of quality and safety have arisen repeatedly and the industry has continued to produce its newest medicines in North America even as technological capabilities increased significantly in China, India, and elsewhere since the 1980s. Compounding pharmacies, by contrast, experienced a steady decline during the second half of the 20th century as medicines arrived from producers in final form. The emergence of individualized therapies, however, is now bringing a resurgence in business for biological labs acting in many ways as compounders. Broadly, as pharmaceutical firms turned to a business model built around research and intellectual property production, and as pharmacists shifted from producing medicines to repackaging them for patients, analysts likewise turned to regulation, pricing, and new product innovation as subjects of their research. As a consequence, pharmaceutical manufacturing is largely ignored in the recent historiography of medicine, pharmacy, and pharmaceuticals, an oversight that should be rectified.
Contributors: Collin, Rachel, Venera-Pontón, Dagoberto E., Driskell, Amy C., Macdonald, Kenneth S., Chan, Kit-Yu Karen, Boyle, Michael J.
... Phoronid larvae, actinotrochs, are beautiful and complicated organisms which have attracted as much, if not more, attention than their adult forms. We collected actinotrochs from the waters of the Pacific and Caribbean coasts of Panama, and used DNA barcoding of mtCOI, as well as 16S and 18S sequences, to estimate the diversity of phoronids in the region. We discovered three operational taxonomic units (OTUs) in the Bay of Panama on the Pacific coast and four OTUs in Bocas del Toro on the Caribbean coast. Not only did all OTUs differ from each other by >10% pairwise distance in COI, but they also differed from all phoronid sequences in GenBank, including the four species for which adults have been reported for the Pacific of Panama, Phoronopsis harmeri, Phoronis psammophila, Phoronis muelleri, and Phoronis hippocrepia. In each ocean region, one common OTU was more abundant and occurred more frequently than other OTUs in our samples. The other five OTUs were relatively rare, with only one to three individuals collected during the entire project. Species accumulation curves were relatively flat but suggest that at least one more species is likely to be present at each site. Actinotrochs from the seven sequenced OTUs had morphologies typical of species with non-brooded planktotrophic development and, in some cases, may be distinguished by differences in pigmentation and the arrangement of blood masses. We found one larva with morphology typical of brooded planktotrophic larvae for which sequencing failed, bringing the total number of potential species detected to eight and representing >50% of the adult species currently recognized globally.
The shape of biogeography: Endemism, maps, and classification of fish distributions in the western Pacific
Contributors: Parenti, Lynne R.
... NH-Vertebrate Zoology