Dataset of allelopathic effects of Casuarina equisetifolia-L leaf aquatic extract on seed germination and growth of selected plant crops
Contributors: Ahmed Abou Elezz
... Casuarina tree is used typically as windbreaks in farms that surrounded by dunes. Moreover, it can play a significant role in inhibiting nearby vegetation growth. In this dataset, Allelopathic effect of Casuarina equisetifolia-L on germination of four selected plant crops (Maize, lentil, Mustard, and Wheat) was studied. Lab and field experiments were conducted to study the allelopathic effects of Casuarina equisetifolia leaf on seed germination and seedling growth. Various concentrations of leaf extract were prepared and used (0, 1.25%, 2.5%, and 5%) in the lab experiment. While in the greenhouse, leaf powder was mixed with soil at concentrations of (0, 15, 30 and 45 g.kg-1 soil). Results revealed that aqueous leaf extract of Casuarina equisetifolia-L affected significantly on seed germination of all selected plant crops under laboratory condition. It was observed that inhibition of 5% was high compared with the control. In addition, the findings showed that leaf extract of Casuarina had a maximum inhibitory effect on root growth more than shoot of the studied seedling. Greenhouse experiments demonstrated that C. equisetifolia exhibited significant allelopathic activity on wheat germination based on all treatments. However, Casuarina leaf extracts presented a positive effect on Mustard germination and growth, while Wheat seeds were negatively affected by all treatments. The pH and the electrical conductivity (EC) of soil were examined with a relative reduction in pH and marked an increase in (EC). Casuarina tree exhibited a relatively negative effect on the selected plant crops.
Contributors: Hui Huang, Lixian Huang, Haibiao Lin
... Biotin as dietary supplement or therapy may lead to analytical interference in biotin-streptavidin immunoassay.When excess biotin is present in the specimen, the biotin molecules will saturate the streptavidin-binding sites, thus preventing the antibody-analyte sandwich from binding with the streptavidin-coated solid phase to generate assay signal after the washing phase. In order to investigate the biotin interference in CanAg Ca242, we compare the difference of experimental samples(spiked biotin solution) and control samples(spiked PBS solution). Pure biotin was dissolved in 0.01 M NaOH and stored at -20°C for reservation. Biotin stock diluted with PBS into working solutions with nine concentration gradients that were then spiked into three different baseline Ca242 samples to achieve the indicated final concentration of biotin (1000,500,250,125,62.5,31.25,15.63,7.81) ng/mL. Our data(Fig .1) shows three different concentration across the analysis of measurement ranges of Ca242 had significant interference from biotin, but the magnitude of the interference was variable at different concentration of biotin. The higher the biotin concentration, the more significant the reduction of Ca242 .The data from table 1 shows that the relative deviation between the baseline level and the biotin-spiked samples, the relative deviation greater than 10% was considered a significant difference according to the indoor quality target. The Ca242-Low level, cutoff level, and high level appeared significant difference respectively at biotin concentration of 15.63 ng/ml, 31.25 ng/ml and 62.5 ng/ml. At the biotin concentration of 250 ng/ml, the relative deviation was more than 99 %（Table 1）. Now that we have confirmed that biotin interferes with Ca242 detection, the next step is to find a feasible solution. Based on the strong affinity to streptavidin, we used streptavidin microparticles to “pre-bound” biotin. The samples were absorbed with magnetic microparticles coated with streptavidin. This reagent included in the Cobas® assays kits supplied by Roche and the streptavidin concentration is 0.72 mg/mL. After pretreatment, the results had recovered firmly to the baseline levels(Table 2).Meanwhile, we performed parallel experiments on the Mindray CL2000i. The data shows that biotin interference does not appear in the Mindray CL2000i intact Ca242 assay, which does not use the streptavidin/biotin method (Fig. 2).
The Data of Isobaric tags for relative and absolute quantification-based proteomic analysis of defense responses triggered by the fungal pathogen Fusarium graminearum in wheat
Contributors: Biao Wang, Xuefeng Li, Wuying Chen, Lingrang Kong
... The raw data for the isobaric tags for relative and absolute quantification-based proteomic analysis of defense responses triggered by the fungal pathogen Fusarium graminearum in wheat. It contained five files.
Contributors: Raúl Roberto poppiel, Marilusa Pinto Coelho Lacerda, José Lucas Safanelli, Rodnei Rizzo, Manuel Pereira de Oliveira junior, Jean Jesus Novais, Jose Alexandre Dematte
... Maps of clay, silt and sand contents (g kg-1) were predicted at 0-20 cm, 20-60 cm and 60-100 cm depths intervals by random forest regression in Google Earth Engine. Gridded soil information covers a part of the Midwest Brazil, from 12° S to 20° S and from 45° W to 54° W, and is available with 250m resolution. The maps were cross-validated and had Coefficient of Determination ranging from 0.64 to 0.85 at all depth intervals.
Antibiotic Properties of Isolated Endophytic Fungi Extracts from Davidson County Community College Campus Flora
Contributors: Joseph Felts, Molly Clark, Harley Hughes, Sopheap Thompson
... These collective data are from projects conducted by the first group of undergraduate research fellows and their advisor at Davidson County Community College (DCCC) in Thomasville, North Carolina. All projects were conducted within a framework of endophytic fungi isolation and testing of organic extracts for antibiotic properties. Individual plant specimens representing 10 species were collected on the college campus. Various tissues were surface sterilized, and then plated on either potato dextrose or yeast-malt extract agar containing kanamycin (50 µg/mL) to inhibit bacterial growth followed by storage at room temperature. Plates were checked regularly for fungal growth and further subsampled and re-plated in an attempt to isolate endophytic fungi. Once a fungus appeared to be isolated, sterilized scalpels were used to remove small strips of agar from a plate and were placed in sterile 15mL conical centrifuge tubes containing sterile water or 250mL flasks containing various type of broth. The 15mL tubes are permanent water stock cultures and are currently stored on the DCCC campus. The 250 mL flasks were used for initial fermentation cultures that were allowed to grow under ambient room conditions with occasional mixing before transfer into 1,000 mL flasks containing more broth. Those flasks were allowed to grow for several weeks with occasional mixing under ambient room conditions before being subjected to organic extraction. Organic extraction consisted of separation of organic and aqueous phases with ethyl acetate. The resulting organic phase was placed in a modified distillation apparatus as there was no access to a rotary evaporator. The ethyl acetate was evaporated off and recollected in a receiving flask and the organic residue remaining from the extraction was collected and stored in methanol in a laboratory freezer until being tested for antibacterial properties. Extracts were tested against four species of bacteria (Escherichia coli, Staphylococcus epidermidis, Bacillus subtilis, and Klebsiella (Enterobacter) aerogenes) using the agar diffusion method in 1:10 potato dextrose: nutrient agar. Plates were inoculated by submerging sterile cotton swabs in fresh broth cultures of a species and subsequent swabbing of agar’s surface. Wells were constructed using sterilized 10mm diameter cuvettes and then filled with 25 µL of extract, or of control (+ kanamycin, - methanol) solutions. Plates were incubated at species appropriate temperatures for 24 hours followed by removal and the measurement (mm) of the zones of inhibition around each well. All testing was done in triplicate. Due to limited time and space, only five organic extractions were completed. Of the five, subsequent statistical analyses indicated four extracts had inhibitory effects against two species, B. subtilis and S. epidermidis while no inhibitory effects against E. coli or K. aerogenes were observed.
Data for: Adherence to non-opioid multimodal analgesia (NOMA) protocol is associated with a shorter length of stay after thoracic surgery
Contributors: Mindaugas Pranevicius, Denise Sullivan, Jody M. Kaban, MD, FACS, Aurimas Knepa, Leonard Golden, Afshin Parsikia, Jack Kuttz
... Study objective: To review the outcomes of non-opioid multimodal analgesia (NOMA) in patients after thoracic surgery. Design: Retrospective one-year cohort. Setting: Single hospital, level one trauma center. Patients. 29 ASA 2-4 patients after thoracotomy (8), open lung biopsy or video-assisted thoracic surgery (VATS) operated by a single surgeon. Interventions: NOMA protocol included intravenous acetaminophen alternating with ketorolac every 3 hours (each every 6 hours, later converted to oral acetaminophen and ibuprofen) with a low dose (0.05mg/kg/h) IV ketamine infusion up to chest tube removal. Opioids were not routinely prescribed from postoperative day one. Measurements: NOMA adherence versus breach (need for >2 opioid rescue doses/24h) rate and it’s association with the perioperative variables and length of stay was explored from the chart review. Main Results: The median (IQR) postoperative opioid requirement was 3 (0-6) doses per hospital stay. 7/29 patients (CI 10-44%) required >2 opioid rescue doses/24 h. Need for opioid rescue was associated with lower BMI and using hydromorphone as oppose to morphine in the recovery room. 3/29 patients were prescribed opioids on discharge. No patients required opioids on follow-up and 63% were pain-free. Median length of stay was 3 (2-5.25) days in NOMA compliant and 9 (6-11) days in opioid rescue groups (P=0.002). In subgroup analysis length of stay reduction was limited to non-thoracotomy patients.
Data for: The PERK/Nrf2 pathway mediates endoplasmic reticulum stress-induced injury by upregulating endoplasmic reticulophagy in H9c2 cardiomyoblasts
Contributors: Xiuhua Liu
... ERS caused ER-phagy with a decrease in cell viability and an increase in cell death in H9c2 cardiomyoblasts.(A) Live/Dead staining of H9c2 cardiomyoblasts was performed using a LIVE/DEAD® Viability/Cytotoxicity Kit (bar=40 μm, n=3). TG- or TM-treated H9c2 cardiomyoblasts were incubated with calcein-AM (green) and EthD-1 (red) for 10 min, and the fluorescence was visualized using a confocal microscope. Green fluorescence indicates live cells, while red fluorescence indicates dead cells. (E) Ultrastructural lesions in the ER caused by TG or TM and the occurrence of ER-phagy were observed via transmission electron microscopy (bar=500 nm). (F) Representative confocal microscopy images corresponding to analysis of ER-phagy for TG- or TM-treated H9c2 cardiomyoblasts and showing colocalization of autophagosomes with ER-fragments (bar=15 μm; n=3). LC3B was labeled with Texas Red, while calreticulin was labeled with Alexa Fluor 488.
Contributors: Pradeep Koulgi, Nicholas Clinton, Krithi Karanth
Contributors: Ling-Ling Chen
... Detailed description of this data can be accessed in Yang et al. Dynamic imaging of RNA in living cells by CRISPR-Cas13 systems.