Contributors: Bradley Dickerson
... Data for Dickerson et al. 2019, "Flies regulate wing motion via active control of a dual-function gyroscope."
Contributors: Gemma Deakin, Adrian Mulligan, Tracey Brown, Emily Jesper-Mir
... Survey data in SPSS and Excel for the Trust and Peer Review survey. This was a global online survey of 3133 active researchers.
Contributors: Nikolai A. Poklonski, Andrei I. Siahlo, Sergey A. Vyrko, Yurii E. Lozovik, Andrei M. Popov
... The file "Figure2-4.m" provides Mathematica code for reproduction of Figures 2-4 of the article. See Figure2-4.pdf for the results of the code execution.
Data for : Voltage-gated potassium channel proteins and stereoselective S-nitroso-L-cysteine signaling
Contributors: Nadzeya Marozkina
... Method 1: L-CSNO binding to proteins after native-PAGE separation The mouse brain membrane fraction (above) was resuspended in HEPES buffer. A BCA (Pierce) protein assay was performed and 50 µg protein was run on two native-PAGE gels (TGX, Bio Rad). One gel was incubated for 30 min in the dark with 50 µM L-CSNO in S-nitrosothiol buffer (10 mM Tris-HCl, pH 6.0, 150 mM NaCl). The second gel was incubated for 30 min in the dark with 50 µM L-CSNO and 100 µM each S-phenyl-cysteine (L-CSφ) and S-methyl-cysteine (L-CSMe) in S-nitrosothiol buffer. Each gel was then rinsed with water and incubated in the dark with 40 µM diaminofluoroscein (DAF) 2 (Cayman Chemicals) for 10 min in the dark (RT) (24). Gels were then imaged on Chemidoc (Bio Rad; Hercules, CA) using the fluorescein setting and bands cut out for mass spectrometry proteomics (see below) that were seen without, but not with, the S-methyl and S-phenyl substituted cysteine co-incubations (21). Method 2: L-CSNO affinity chromatography L-CSNO affinity columns were prepared as follows. L-Cysteine was coupled to AminoLink Plus (Pierce/ThermoFisher; Waltham, MA) resin according to the manufacturer’s protocol. In control experiments, aminoLink Plus was used without reaction with L-cysteine. Briefly, L-cysteine was dissolved in coupling buffer A (0.1 M sodium citrate, 0.05 M sodium carbonate, pH 10) at a concentration of 100 mM and incubated with 0.4 ml, previously washed, AminoLink resin in a small (0.5 ml) column for 4 hours at room temperature. The resin was then washed with coupling buffer B (0.1 M phosphate. 0.15 M sodium chloride, pH 7.2) and incubated for 4 hours at room temperature with 100 mM cyanoborohydride in coupling buffer B. Active sites were then blocked with quenching buffer (1 M Tris-HCl, pH 7.4). Resin was then washed with 1 M NaCl and then incubated for 10 min with 30% ethyl nitrite (EtONO) in ethanol at room temperature in the dark (25). It was then washed with 100% ethanol followed by 1M NaCl. Excised mouse brain homogenate was briefly centrifuged and the supernatants were then incubated with prepared Aminolink columns for 30 min at room temperature. The columns were then washed with wash buffer and bound protein eluted with Laemmli buffer and elution buffer (0.1 M Glycine, pH 3.5). Eluate was concentrated and used for SDS-PAGE gel (TGX, BioRad; Hercules, CA) followed by Coomassie staining. Bands found in samples from + L- Cysteine columns but not in – L-cysteine columns were excised and analyzed further by MS proteomics. Biotin substitution analysis for NO-substituted cysteine’s in Kv proteins CHO cells expressing all three Kv proteins were plated on in 6 wells of a six well plate. Once confluent, cells were incubated with no treatment, 500 µM L-CSNO, or 500 µM L-CSNO plus 10mM leucine for two minutes. Wells were then washed with cold PBS and lysed in HEN buffer (250mM HEPES pH7.7, 1mM EDTA, 0.1mM Neocuproine). After biotin switch, samples went to LC-MS.
Contributors: Boda Liu
... We provide the original data and visualization scripts associated with "Importance of the size and distribution of chemical heterogeneities in the mantle source to the variations of isotope ratios and trace element abundances in mid-ocean ridge basalts" GCA2019
Contributors: Huajun Ming
... The Fredlund-Xing (FX) model was chosen to develop the proposed equation. 1. SWR data sets were recoreded from other published papers. Related references can be found in Table 3. 2. Tables and Figures are shown in the paper. 3. Original datum of figures can be found in the folder "OriginPro and pictures related to Figures", and original pictures of figures can also be found in the jpg type.
A Mathematical Model for the Berth Allocation Problem with Machine Patterns and Continuous Time Horizon
Contributors: Bruno Luís Hönigmann Cereser
... Intances, codes and results.
Contributors: Constance Schmidt, Stephen Schmidt, Kara Wilson
... Word Lists for Experiments 1 through 3. SPSS data files for Experiments 1 through 3. T-test data file for Experiment 3. SPSS files include recall as a function of condition in each experiment.
Contributors: Enrique Garcia-Macias, Alejandro E. Martínez-Castro
... All the data relating the case studies used to validate the HTSA approach. These include: simply supported beam, continuous three-span bridge, the viaduct of Rodenillo, and the viaduct of Santa Ana. Along with the data files, MATLAB scripts are also provided to post-process the results.
Data for: Transient basin as an indicator of tectonic expressions in bedrock landscape: an approach based on MATLAB geomorphic tool (Transient profiler)
Contributors: Prabha Pandey
... Transient Profiler (Matlab tool)