Contributors: Javier Murillo, Ellen Kenchington, Gabrielle Tompkins-MacDonald, Lindsay Beazley, Emily Baker, Anders Knudby, Wojciech Walkusz
... Little information exists on the environmental requirements of sponges from the Canadian Arctic, increasing the necessity to establish baseline distribution data on sponge as - semblages to predict their susceptibility to climate change. Here we describe the sponge taxa of Hudson Strait, Ungava Bay, Western Davis Strait and Western Baffin Bay collected by Canadian research vessel trawl surveys. A total of 2026 sponge specimens were examined, and 93 different taxa were identified with 79% identified to species, of which 2 are new to science, 1 recorded for the first time in the North Atlantic, 16 are new records for the northwest Atlantic, and 10 are new records for the Baffin Bay, Davis Strait and Hudson Strait sponge fauna. Taxonomic distinctness was higher north of Cape Dyer and south of Davis Strait, whereas the number of species reached a maximum in Davis Strait, which represents the southern distribution limit of the arctic sponge fauna along the slope in this region. Five sponge species assemblages were identified, some of which have been observed elsewhere, suggesting that they may be common to the North Atlantic and at the generic level to the global oceans. Two of the Baffin Bay−Davis Strait assemblages were characterized by large structure-forming astrophorids: one, with arctic species, found at midwater depths in Baffin Bay and the other, characterized by boreal species, was found deeper, south of Davis Strait. Another assemblage characterized by glass and carnivorous sponges was found along the continental slope of western Baffin Bay. Candidate target indicator species are provided for future sponge community monitoring.
Contributors: Guillermo Reyes
... SEM, AFM, Conductometric and optical images for cellulose dissolution and hydrolysis
Essential gene profiles for human pluripotent stem cells identify uncharacterized genes and substrate dependencies. Mair et al.
Contributors: Barbara Mair, Jelena Tomic, Sanna Masud, Jason Moffat
... Human pluripotent stem cells (hPSCs) provide an invaluable tool for modeling diseases and hold promise for regenerative medicine. For understanding pluripotency and lineage differentiation mechanisms, a critical first step involves systematically cataloging genes that are indispensable for hPSC maintenance and proliferation. To map genetic determinants of hPSC fitness, we performed genome-scale loss-of-function screens in an inducible Cas9 H1 hPSC line cultured on feeder cells and feeder-free to identify essential genes. Among these, we found FOXH1 and VENTX, genes that encode transcription factors previously implicated in stem cell biology, as well as an uncharacterized gene, C22orf43/DRICH1. hPSCs essential genes are substantially different from other cell lines, and gene essentiality in hPSCs is highly context-dependent with respect to different growth substrates. Our CRISPR screens establish parameters for genome-wide screens in hPSCs, which will facilitate the characterization of unappreciated regulators of hPSC biology to understand the genetic wiring of hPSC fitness and pluripotency.
Strain effect of hydrogen evolution reaction of single-layer molybdenum disulfide containing molybdenum vacancies：A DFT study
Contributors: KongQiang Ye
... In this paper, the hydrogen evolution catalytic properties of single-layer molybdenum disulfide containing single molybdenum defects were studied by first-principles calculation, and the effects of different strains (uniaxial stretching, biaxial stretching, biaxial compression) on them were studied. The data in this paper have the bond length and bond angle of molybdenum disulfide under different strains, as well as electronic properties analysis (energy band, density of states, bader charge analysis, etc.)
Activity, selectivity and characterization data of nano-shaped CeO2 supports for carbon monoxide removal from an actual syngas stream
Contributors: Bernay Cifuentes, Martha Cobo Angel, Felipe Bustamante
... This dataset contains the information of activity, selectivity and stability tests of carbon monoxide removal from syngas using AuCu catalysts supported on different nano-shaped ceria (i.e., polyhedra, rods and cubes). CeO2 without defined morphology is also included. Characterization data of EDS, Raman spectroscopy, XRD, TEM, and TGA are also included.
Data for: Experimental tests and post-test analysis of non-uniformly heated 19-pins fuel bundle cooled by Heavy Liquid Metal
Contributors: Ranieri Marinari, Morena Angelucci, Daniele Martelli, Ivan Di Piazza, Mariano Tarantino
... The two .txt files (ADP_06 and ADP_07) contain the raw experimental data. TC nomenclature.docx file contains the nomenclature and location of the termocouples inside the Fuel Pin bundle simulator (test section). The other files contains the matlab scripts adopted for post-processing the raw data files.
Contributors: Alexis Huf, Frank Siqueira
... This repository contains document data for a Systematic Literature Review (SLR) titled "Composition of Heterogeneous Web Services: A Systematic Review". Inclusion/exclusion decision and extracted data from the documents are included. Three main types can be identified on the Web and on corporate networks: SOAP services, which use the homonym protocol and well established technologies, such as WSDL; RESTful services which employ HTTP directly and conform to the constraints of the REST architectural style; and event-oriented services that take the initiative in notifying their clients about relevant facts. The co-existence of these service types has brought considerable research interest on service type heterogeneity in Web Service composition. The research question of SLR is "How are services of heterogeneous types (SOAP, RESTful and \event-oriented services) composed?". Documents that may answer this question were searched in Scopus and IEEE Xplore, from conferences and journal sources, without a time limit. Search results were last updated in July 22, 2018 and yielded 63 relevant documents published from 2005 to 2018. Most works (48) target SOAP and RESTful services heterogeneity, followed by a smaller group of 18 targeting SOAP and event-oriented heterogeneity. The other two combinations, RESTful/event-oriented and SOAP/RESTful/event-oriented sum 5 documents. Among these documents, RESTful support was found to be incipient, with most documents violating constraints of the REST architectural style. The method used for heterogeneity support were classified in 7 archetypes: 1. Common description 2. Proxy 3. Middleware 4. Workflow language 5. Event processor 6. Automatic composition 7. Direct Implementation
Contributors: Xiaolei Nie
... In this study we endeavoured to develop a full-scaled osteochondral graft with LhCG as the cartilage phase, PLGA scaffold as the subchondral phase, and a naturally formed interpentration network (IPN) that mimics the cartilage-bone interface. The IPN in the graft is formed by spontaneous migration of chondrocytes in LhCG into the porous PLGA scaffold. The decellularization potential of the full-scaled osteochondral graft will also be investigated in this study. Collectively we have developed both cell-laden and decellularized full-scaled tissue engineered graft with a naturally formed cartilage-bone interpenetration zone and investigated their potential to repair traumatic osteochondral lesions. The anisotropic graft is characterized for its microarchitecture and biochemical composition. The osteochondral lesion repair capability was investigated within a rabbit osteochondral defect model by examining the mechanical property and biological property of the regenerated tissue. The PLGA-SMS subchondral layer was integrated with scaffold-free cartilage layer sandwiching a transition zone to establish the cartilage-bone interface. Gelation of chondral layer on top of the porous subchondral bone layer mimics the osteochondral gradient in terms of biochemical composition and its micro-architecture. The secretion of ECM from native chondrocytes and the migration of chondrocytes into the porous SMS scaffold formed the layer specific osteochondral tissue zonal structure naturally. The decellularized version of the graft has been successfully derived as confirmed by the removal of cellular component and retention of ECM composition and its morphology. In the untreated defect no regeneration was observed in the cartilage layer and the tissue gradually degenerated. On the other hand, the biomimetic gradient LhCG+ scaffold exhibited superior tissue coverage at the defect macroscopically. Furthermore, histological analysis demonstrated the regeneration of both subchondral bone and cartilage tissue as evidenced by the same phenotype of neo-tissue when compared with the native healthy osteochondral tissue in the same zonal region. In addition, confined subchondral bone formation with bone volume to total defect volume similar to the native tissue was observed in the LhCG+ graft after 100 days of implantation within the animal model studies. Among the two types of graft, LhCG+ demonstrated a significantly higher Young’s modulus which suggested stronger load-bearing capacity of regenerated neo–tissue which is approximately 70% of native tissue mechanical strength. These results suggested that the engineered osteochondral mimetic cell laden full-scaled graft that comprises of cartilage layer, subchondral bone layer and gradient interface showed strong regenerative capabilities to repair osteochondral tissue defects.
Contributors: M. Florencia Martini
... Files of DMPC-melittin and DMPC-pentalysin interaction, and different types of data analysis
Data for: Characterizations on the microstructure and micro-mechanics of cast Be-Al-0.4Sc-0.4Zr alloy prepared by vacuum induction melting
Contributors: Liangbo Yu
... The data corresponding to the figures in the manuscript can be distinguished from their filenames.