235 results for qubit oscillator frequency
Contributors: Carmen Stavarache, B. Yim, M. Vinatoru, Y. Maeda
... The influence of ultrasound (200kHz) on the decomposition of water solution (around 100 ppm) of chlorobenzene in the presence and absence of iron and palladium sulfates was investigated. The intermediates of sonolysis were identified, leading to a deeper insight on the degradation mechanism. It was established that chlorobenzene, a volatile compound, is degraded mainly by pyrolysis inside cavitation bubbles. The first sonolysis product is benzene, formed, by an outside cavitation reaction, from phenyl radicals and hydrogen atoms, sonolytically generated from water. Polyphenolic compounds, as chlorobenzene sonochemical degradation, are reported. Alternative mechanism to chlorobenzene sonolysis is advanced to explain phenols, chlorophenols and benzene formation. This work brings some light on the reaction sites and decomposition mechanisms during sonication of chlorobenzene. Keywords: chlorobenzene, sonolysis, sonochemical mechanism, waste water treatment.
The dominant 55kDa allergen of the subtropical Bahia grass (Paspalum notatum) pollen is a group 13 pollen allergen, Pas n 13
Contributors: Janet M. Davies, Astrid Voskamp, Thanh D. Dang, Benjamin Pettit, Dorothy Loo, Arnd Petersen, Michelle M. Hill, John W. Upham, Jennifer M. Rolland, Robyn E. O’Hehir
... Bahia grass, Paspalum notatum, is an important pollen allergen source with a long season of pollination and wide distribution in subtropical and temperate regions. We aimed to characterize the 55kDa allergen of Bahia grass pollen (BaGP) and ascertain its clinical importance. BaGP extract was separated by 2D-PAGE and immunoblotted with serum IgE of a grass pollen-allergic patient. The amino-terminal protein sequence of the predominant allergen isoform at 55kDa had similarity with the group 13 allergens of Timothy grass and maize pollen, Phl p 13 and Zea m 13. Four sequences obtained by rapid amplification of the allergen cDNA ends represented multiple isoforms of Pas n 13. The predicted full length cDNA for Pas n 13 encoded a 423 amino acid glycoprotein including a signal peptide of 28 residues and with a predicted pI of 7.0. Tandem mass spectrometry of tryptic peptides of 2D gel spots identified peptides specific to the deduced amino acid sequence for each of the four Pas n 13 cDNA, representing 47% of the predicted mature protein sequence of Pas n 13. There was 80.6% and 72.6% amino acid identity with Zea m 13 and Phl p 13, respectively. Reactivity with a Phl p 13-specific monoclonal antibody AF6 supported designation of this allergen as Pas n 13. The allergen was purified from BaGP extract by ammonium sulphate precipitation, hydrophobic interaction and size exclusion chromatography. Purified Pas n 13 reacted with serum IgE of 34 of 71 (48%) grass pollen-allergic patients and specifically inhibited IgE reactivity with the 55kDa band of BaGP for two grass pollen-allergic donors. Four isoforms of Pas n 13 from pI 6.3–7.8 had IgE-reactivity with grass pollen allergic sera. The allergenic activity of purified Pas n 13 was demonstrated by activation of basophils from whole blood of three grass pollen-allergic donors tested but not control donors. Pas n 13 is thus a clinically relevant pollen allergen of the subtropical Bahia grass likely to be important in eliciting seasonal allergic rhinitis and asthma in grass pollen-allergic patients.
Contributors: K. Yamada, H. Hoshikawa, S. Maki, T. Ozaki, Y. Kuroki, S. Kamado, Y. Kojima
... The addition of 0.25–0.5at.% of Ag to Mg–3.4Gd–0.1Zr (at.%) alloy significantly enhances the age-hardening response, particularly in underaged conditions. The peak-aged Mg–3.4Gd–0.5Ag–0.1Zr alloy exhibits an extremely high ultimate strength, of above 410MPa. The plate precipitates are formed on the basal plane of the matrix with a high number density in underaged conditions and co-exist with β′ phase in the peak-aged condition. The co-precipitation effectively contributes to the strengthening of the alloy. In the Ag-added Mg–Gd alloys, characteristic initial structure change is found.
Identification and expression analysis of cold and freezing stress responsive genes of Brassica oleracea
Contributors: Nasar Uddin Ahmed, Hee-Jeong Jung, Jong-In Park, Yong-Gu Cho, Yoonkang Hur, Ill-Sup Nou
... Cold and freezing stress is a major environmental constraint to the production of Brassica crops. Enhancement of tolerance by exploiting cold and freezing tolerance related genes offers the most efficient approach to address this problem. Cold-induced transcriptional profiling is a promising approach to the identification of potential genes related to cold and freezing stress tolerance. In this study, 99 highly expressed genes were identified from a whole genome microarray dataset of Brassica rapa. Blast search analysis of the Brassica oleracea database revealed the corresponding homologous genes. To validate their expression, pre-selected cold tolerant and susceptible cabbage lines were analyzed. Out of 99 BoCRGs, 43 were differentially expressed in response to varying degrees of cold and freezing stress in the contrasting cabbage lines. Among the differentially expressed genes, 18 were highly up-regulated in the tolerant lines, which is consistent with their microarray expression. Additionally, 12 BoCRGs were expressed differentially after cold stress treatment in two contrasting cabbage lines, and BoCRG54, 56, 59, 62, 70, 72 and 99 were predicted to be involved in cold regulatory pathways. Taken together, the cold-responsive genes identified in this study provide additional direction for elucidating the regulatory network of low temperature stress tolerance and developing cold and freezing stress resistant Brassica crops.
Original article - Multi-locus genotyping reveals absence of genetic structure in field populations of the brown ear tick (Rhipicephalus appendiculatus) in Kenya
Contributors: Esther G. Kanduma, Joram M. Mwacharo, Stephen Mwaura, Joyce N. Njuguna, Inosters Nzuki, Peter W. Kinyanjui, Naftaly Githaka, Heloise Heyne, Olivier Hanotte, Robert A. Skilton
... Rhipicephalus appendiculatus is an important tick vector of several pathogens and parasitizes domestic and wild animals across eastern and southern Africa. However, its inherent genetic variation and population structure is poorly understood. To investigate whether mammalian host species, geographic separation and resulting reproductive isolation, or a combination of these, define the genetic structure of R. appendiculatus, we analyzed multi-locus genotype data from 392 individuals from 10 geographic locations in Kenya generated in an earlier study. These ticks were associated with three types of mammalian host situations; (1) cattle grazing systems, (2) cattle and wildlife co-grazing systems (3) wildlife grazing systems without livestock. We also analyzed data from 460 individuals from 10 populations maintained as closed laboratory stocks and 117 individuals from five other species in the genus Rhipicephalus. The pattern of genotypes observed indicated low levels of genetic differentiation between the ten field populations (FST=0.014±0.002) and a lack of genetic divergence corresponding to the degree of separation of the geographic sampling locations. There was also no clear association of particular tick genotypes with specific host species. This is consistent with tick dispersal over large geographic ranges and lack of host specificity. In contrast, the 10 laboratory populations (FST=0.248±0.015) and the five other species of Rhipicephalus (FST=0.368±0.032) were strongly differentiated into distinct genetic groups. Some laboratory bred populations diverged markedly from their field counterparts in spite of originally being sampled from the same geographic locations. Our results demonstrate a lack of defined population genetic differentiation in field populations of the generalist R. appendiculatus in Kenya, which may be a result of the frequent anthropogenic movement of livestock and mobility of its several wildlife hosts between different locations.
Brief report - PIKK-dependent phosphorylation of Mre11 induces MRN complex inactivation by disassembly from chromatin
Contributors: Michela Di Virgilio, Carol Y. Ying, Jean Gautier
... The role of Mre11 phosphorylation in the cellular response to DNA double-strand breaks (DSBs) is not well understood. Here, we show that phosphorylation of Mre11 at SQ/TQ motifs by PIKKs (PI3 Kinase-related Kinases) induces MRN (Mre11–Rad50–Nbs1) complex dissociation from chromatin by reducing Mre11 affinity for DNA. Whereas phosphorylation of Mre11 at these residues is not required for DSB-induced ATM (Ataxia-Telangiectasia mutated) activation, abrogation of Mre11 dephosphorylation impairs ATM signaling. Our study provides a functional characterization of the DNA damage-induced Mre11 phosphorylation, and suggests that MRN inactivation participates in the down-regulation of damage signaling during checkpoint recovery following DSB repair.
Contributors: Margarete Digel, Robert Ehehalt, Joachim Füllekrug
Live microscopy of lipid droplets (Powerpoint file). This short animation visualizes the main kinds of movement which have been observed. Most of the LDs undergo only limited oscillations (e.g. within the grey circle). In the brown circle there is one LD jumping spontaneously. Within the orange circle, two small dots meet and immediately depart again. The lower still image compares the position of LDs at the beginning of the recording time (red) with their final destination (green). Most of the bigger LDs are yellow suggesting that they did not move during the time of observation. The small yellow arrow indicates the saltatory movement of the LD within the red circle ... Lipid droplets emerge as important intracellular organelles relevant for lipid homeostasis and the pathophysiology of metabolic diseases. Here, we present a personal view on the current knowledge about the biogenesis of mammalian cytoplasmic lipid droplets, with a focus on microscopy and especially live imaging. We also discuss difficulties related to the lipid droplet proteome, contentious views on lipid droplet growth, and last but not least the evidence for the heterogeneity of lipid droplets within a single cell. We conclude with an outline of the most important future challenges.
Contributors: Tomohisa Asai, Eriko Sugimori, Naoko Bando, Yoshihiko Tanno
The relative frequency distributions of each total schizotypy scores (combined among groups 0–4). ...The relative frequency distributions of each total schizotypy scores (combined among groups 0–4). ... Schizotypal personality traits (schizotypy) might be seen as on a continuum with schizophrenia. However, controversy remains with regard to whether this continuum is quasi-dimensional, applying only to people with schizophrenia and schizotypy, or fully dimensional, applying to all people. If the fully dimensional model is accurate, schizotypy could be described by the same personality theories as are applied to people in general. We examined the relationship between schizotypy and the five-factor model of personality (FFM), which is arguably the most established contemporary personality theory. When we assumed a hierarchic structure of schizotypy factors, we found that the FFM scales could explain schizotypy fairly well regardless of the questionnaires used, suggesting that schizotypy might represent a variation better understood by reference to typical dimensions of personality, though it might still indicate a predisposition to schizophrenia. This article discusses this conclusion in relation to each of the five personality factors. A perspective that situates schizophrenia on a continuum with general personality variations implies that this disorder constitutes a potential risk for everyone and, thus, helps to promote understanding and correct misunderstandings that contribute to prejudice.
Proteomic analysis of plasma samples from patients with acute myocardial infarction identifies haptoglobin as a potential prognostic biomarker
Contributors: Benjamin Haas, Tommaso Serchi, Daniel R. Wagner, Georges Gilson, Sebastien Planchon, Jenny Renaut, Lucien Hoffmann, Torsten Bohn, Yvan Devaux
Plasma Hp levels in 30 acute MI patients. (A) Frequency plot. The dotted line indicates the 2g/L threshold for abnormally high Hb values. (B) The 3 groups of patients of the test cohort showed different Hp plasma levels. Means±95% confidence intervals are shown. ...Plasma Hp levels in 30 acute MI patients. (A) Frequency plot. The dotted line indicates the 2g/L threshold for abnormally high Hb values. (B) The 3 groups of patients of the test cohort showed different Hp plasma levels. Means±95% confidence intervals are shown. ... Prognosis of clinical outcome following myocardial infarction is variable and difficult to predict. We have analyzed the plasma proteome of thirty patients with acute myocardial infarction to search for new prognostic biomarkers. Proteomic analyses of blood samples were performed by 2-D-DiGE after plasma depletion of albumin and immunoglobulins G. New York Heart Association (NYHA) class determined at 1-year follow-up was used to identify patients with heart failure. Principal component analysis and hierarchical clustering of proteomic data revealed that patients could be separated into 3 groups. The 22 differentially expressed proteins involved in this grouping were identified as haptoglobin (Hp) and respective isoforms. The 3 groups of patients had distinct Hp isoforms: patients from group 1 had the α1–α1, patients from group 2 the α2–α1, and patients from group 3 the α2–α2 genotype. This classification was also associated with different total plasma levels of Hp. The presence of the α2 genotype and low plasma levels of Hp was associated with a higher NYHA class and therefore with a detrimental functional outcome after myocardial infarction. A plasma level of Hp below 1.4g/L predicted the occurrence of heart failure (NYHA 2, 3, 4) at 1-year with 100% sensitivity.
Letter to the Editor - Re: “Increased Langerhan cell density and corneal nerve damage in diabetic patients: Role of immune mechanisms in human diabetic neuropathy” by Tavakoli et al.
Contributors: Andrey Zhivov, Oliver Stachs