2026-10-14T14:39:15Z https://data.mendeley.com/oai

oai:data.mendeley.com/2kvc98hc65.2 2025-10-29T16:38:11Z

Burton, Oliver AutoSpectral: 40-colour ID7000 dataset Mendeley Data Healthy human PBMC and buffy coat samples, cryopreserved and stained with a 40-colour flow cytometry panel. Acquired on a 5-laser Sony ID7000 spectral instrument. Unmixed using WLSM on the Sony software or using AutoSpectral. WLSM: Sony software unmixed using weighted least squares WLS: AutoSpectral unmixed using weighted least squares scAF: AutoSpectral unmixed using weighted least squares and per-cell (single cell) autofluorescence extraction perCellFluor: AutoSpectral unmixed using weighted least squares, per-cell autofluorescence extraction and per-cell fluorophore spectral optimization. Slow = exact recomputation of the unmixing matrix per variant, Medium = rank-one updating, Fast = approximation using the difference in spectrum between variants and the abundance of the endmember (fluorophore). Flow Cytometry Spectral Unmixing Buecken, Leoni De Vuyst, Leana Dooley, James Liston, Adrian Humblet-Baron, Stephanie Dataset doi:10.17632/2kvc98hc65.2 oai:data.mendeley.com/2kvc98hc65.2 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/2kvc98hc65 2025-10-29T16:38:11Z