2026-10-15T21:05:51Z https://data.mendeley.com/oai

oai:data.mendeley.com/3fbrj2zgjw.1 2025-01-03T07:38:44Z

Wang, lina Alpine grassland degradation mediates soil bacterial community assembly processes: a perspective on life-history strategies trade-offs Mendeley Data This study investigated the impact of grassland degradation on plant community structure, soil properties, bacterial communities across five degradation levels (non-degraded, lightly degraded, moderately degraded, severely degraded and extremely degraded) in alpine meadows of the Three Rivers Source Area region on the Qinghai-Tibetan Plateau. Soil sampling was conducted following the plant community survey. At each site, five surface soil samples (0-10 cm) were randomly collected and then combined to form a single composite sample. Some soil samples were tested for soil physical and chemical properties including soil microbial biomass carbon, microbial biomass nitrogen, urease , invertase and alkaline phosphatase, soil pH, soil organic carbon, soil total nitrogen, soil total phosphorus, soil ammonium nitrogen, soil nitrate nitrogen, soil available phosphorus, and soil moisture content. And high-throughput gene sequencing was performed with the Illumina PE250 sequencing platform (Illumina, San Diego, CA, USA). The primers 515F(5’-GTGCCAGCMGCCGCGG-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’) were used for amplification of the V3-V4 region of the bacterial 16S rRNA gene.PCR amplification products were performed in a TransStart Fastpfu DNA Polymerase, 20 μl reaction system, PCR machine is ABI GeneAmp® 9700, all experimental samples are carried out according to formal experimental requirements, and each experimental sample is repeated three times. Soil Ecosystem Degradation Plant Community Bacterial Community Dataset doi:10.17632/3fbrj2zgjw.1 oai:data.mendeley.com/3fbrj2zgjw.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/3fbrj2zgjw 2025-01-03T07:38:44Z