2026-10-15T11:46:17Z https://data.mendeley.com/oai

oai:data.mendeley.com/3rptcr8rxj.1 2026-09-06T22:02:34Z

Yu, Linyang Punicalagin inhibits Getah virus replication by directly blocking viral attachment and internalization Mendeley Data Getah virus (GETV) is an important mosquito-borne zoonotic pathogen, like Chikungunya virus (CHIKV), belonging to the genus Alphavirus of the family Togaviridae. Since 2024, the highly virulent GETV GIII variant strain has emerged in China, causing severe abortions in sows and piglet mortality rates as high as 80%, resulting in substantial economic losses to the swine industry. However, no approved vaccines or effective antiviral drugs are currently available for the prevention and control of GETV. In this study, we screened a library of 113 natural compounds using a GETV-GFP reporter strain and identified 14 candidate molecules with anti-GETV activity. Among them, punicalagin (PUN) showed the most potent antiviral activity. In vitro experiments demonstrated that PUN exhibited significant dose-dependent inhibitory effects against multiple GETV strains, including the GETV-GFP strain, the highly virulent GIII variant strain HeN01 and the non-variant GIII strain HNny25. Mechanistic studies revealed that PUN blocks the viral attachment and internalization stages to exert its antiviral action. In addition, we found that PUN significantly inhibited the replication of other enveloped viruses, including porcine epidemic diarrhea virus (PEDV), pseudorabies virus (PRV), and vesicular stomatitis virus (VSV), but showed no activity against the non-enveloped virus Senecavirus A (SVA). Furthermore, in suckling mice challenged with the highly virulent HeN01 strain, PUN treatment significantly increased the survival rate to 66.7% (4/6), ameliorated growth and development indicators, and effectively reduced the viral load in major target organs. These findings not only provide a solid theoretical basis for the future clinical translation of PUN but also hold great promise in bridging the gap in specific GETV therapeutics. Virus Antivirus Agent Geng, Yidan Yang, Zhengxin Li, Lanlan Liu, Chao Gao, Fei Li, Dongliang Li, Jianli Zheng, Guanmin Chen, Lu Li, Wentao Du, Wenjuan Li, Yongtao Dataset doi:10.17632/3rptcr8rxj.1 oai:data.mendeley.com/3rptcr8rxj.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/3rptcr8rxj 2026-09-06T22:02:34Z