2026-10-15T21:04:28Z https://data.mendeley.com/oai

oai:data.mendeley.com/49smydnjjn.4 2026-10-08T10:21:23Z

Wu, Zongcheng Data supporting an auditable sparse-evidence workflow for phage receptor-binding-protein prioritization and Gp17-mediated magnetic capture of Yersinia enterocolitica O:3 Mendeley Data Version 4 adds an ELISA experimental-unit correction and reanalysis record for the Journal of Biotechnology manuscript. No experiments were added. Frozen 827-record scores, weights, rankings and seeds are unchanged. V3 and earlier files remain as historical provenance. The authors confirmed that each of the three stored values per condition in the binding and specificity workbooks is the mean of three technical wells from one independent experiment. Thus n = 3 independent experiments. Individual well-level values are unavailable and were not reconstructed. V3's description of "36 technical wells" and its single-assay interpretation are superseded for the current analysis. Separate binding fits give apparent parameters of 41.21, 46.37 and 45.67 micrograms/mL: 44.41 +/- 2.80 micrograms/mL (mean +/- between-experiment sample SD). This mass-concentration parameter concerns the tagged preparation, not intrinsic molar affinity. Historical 44.38 +/- 2.98 (estimate +/- SE; R-squared 0.9904) remains an earlier analysis; its SE must not be relabelled SD. Binding uses the unrounded mean of recorded negative values. Specificity uses matched experiment rows and each group's negative-control mean, with paired two-sided tests and Holm correction. Small n limits assumptions. The corrected outputs, source workbooks and analysis code were already published in v1.1.0, https://doi.org/10.5281/zenodo.23156488, under extension_v04/04_ELISA, extension_v04/01_source_snapshot/ELISA and extension_v04/analysis/analyze_elisa.py. The added JoB_reanalysis_correction_20261006.zip contains 09_JoB_reanalysis_20261006: corrected derived tables, summary, ELISA figure, source hashes and figure crosswalk. It does not replace code. Historical software v1.0.0 remains at https://doi.org/10.5281/zenodo.22625899. Software and third-party licences remain separate. The retrospective evidence extensions distinguish 827 accession records from 744 unique sequences and examine identity, missing evidence and receptor/structure input requirements. They do not establish independent predictive accuracy, complete external prediction or cross-target generalization. The functional case concerns tagged φYeO3-12 Gp17, distinct from prior AP5-derived Gp17 magnetic-capture work. The historical 87.2% count index lacks recoverable volume/dilution normalization and is not absolute recovery. Food-extract observations are not a surface-inoculation challenge or validated detection limit. Current JoB Figure 4 (ELISA) corresponds to historical V3 Figure 3. Consult the new correction README and figure crosswalk before combining versions. Original source and image-provenance limitations remain applicable. Food Microbiology Bacteriophage Receptor Binding Foodborne Pathogens Ren, Honglin Shangguan, Chunyi Li, Yansong Lu, Han Zhou, Xiangyu Jiang, Kang Wang, Ziyi Hu, Pan Liu, Xilin Lu, Shiying Dataset doi:10.17632/49smydnjjn.4 oai:data.mendeley.com/49smydnjjn.4 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/49smydnjjn 2026-10-08T10:21:23Z