2026-10-12T02:50:37Z https://data.mendeley.com/oai

oai:data.mendeley.com/5ngx4jz7fg.1 2025-07-11T09:24:07Z

li, runliu Data Repository: An aptasensor for specific quantification of the signaling protein Amuc_1100 from Akkermansia muciniphila MucT Mendeley Data The combination of rGO-FETs (reduced Graphene Oxide-Field Effect Transistor) and DNA oligonucleotide aptamers has proved to be a promising technical method to detect analytes with high sensitivity and specificity. Targeting the Amuc_1100 protein, we demonstrate the application and ability of the aptamer/FET combination to detect Akkermansia muciniphila, an intestinal symbiont that improves host metabolism. Amuc_1100, a protein highly expressed on the bacterial outer membrane that signals to Toll-like receptor 2 and, has been shown to partially replicate these beneficial effects. By developing, characterizing, and demonstrating the suitability of SELEX based anti-AMUC_1100 aptamer libraries for the construction of functional rGO-FET sensors for specific and sensitive Amuc_1100 quantification, we provide evidence that the evolution of focused polyclonal aptamer libraries for related targets can be used as a straightforward strategy. Revolution and innovation in sensing technology will undoubtedly further improve the diagnostic and monitoring process in biomedical technlogy. Field Effect Transistor Aptamer Microorganism Kissmann, Ann-Kathrin Dataset doi:10.17632/5ngx4jz7fg.1 oai:data.mendeley.com/5ngx4jz7fg.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/5ngx4jz7fg 2025-07-11T09:24:07Z