2026-10-15T23:28:41Z https://data.mendeley.com/oai

oai:data.mendeley.com/65pfcj4j4m.1 2023-10-24T01:46:48Z

Yang, Xin Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators Mendeley Data Ferroptosis, an iron-dependent programmed cell death triggered by excessive lipid peroxidation, has shown promising therapeutic potentials in human diseases. Here, we describe a protocol of a CRISPR-Cas9 loss-of-function screen to identify regulators in response to different inducers of ferroptosis. We emphasize the steps of library amplification, drug treatment, high-throughput sequencing preparation, and bioinformatics analysis using Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout (MAGeCK). We also present a method to discover the regulators of ferroptosis and verify the potential targets efficiently. CRISPR/Cas9 Ferroptosis Gu, Wei Dataset doi:10.17632/65pfcj4j4m.1 oai:data.mendeley.com/65pfcj4j4m.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/65pfcj4j4m 2023-10-24T01:46:48Z