2026-10-12T14:00:48Z https://data.mendeley.com/oai

oai:data.mendeley.com/68k3bsz2tz.1 2025-10-10T14:01:13Z

Cui, Zekai Single-Cell Transcriptomics of Nfe2l2-KO versus WT Mouse Corneas Mendeley Data Fresh corneas from WT and Nfe2l2-KO mice at P14/P28 (n = 5/group, 4-month-old) were dissociated with papain (20 U/mL) + DNase I for 15–20 min at 37 °C, quenched with ovomucoid, filtered (40 µm), and processed on a droplet-based scRNA-seq platform. Library preparation and sequencing were outsourced to Singleron Biotechnologies (Nanjing, China). Raw reads were aligned to mm10 with STAR/Drop-seq Tools; ambient RNA was corrected with SoupX. In Seurat v5, low/high feature outliers and high-mitochondrial cells were removed, doublets were filtered (DoubletFinder), SCTransform normalization and integration were applied, clusters were identified by SNN/UMAP, and annotated with canonical markers. Differential expression used Wilcoxon with BH FDR < 0.05. Functional enrichment of DEGs was performed with Metascape. Cell–cell communication was inferred with CellChat v1.6 (MouseDB; 1,000 permutations; FDR < 0.05) and summarized at pathway level. Cornea Keratoconus Single-Cell RNA Sequencing Transcription Factor Nrf2 Dataset doi:10.17632/68k3bsz2tz.1 oai:data.mendeley.com/68k3bsz2tz.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/68k3bsz2tz 2025-10-10T14:01:13Z