2026-10-15T11:45:04Z https://data.mendeley.com/oai

oai:data.mendeley.com/7mncdjvwpb.1 2025-02-20T18:36:10Z

Guan, tangfei Exploring the Hair Growth-Promoting Effects of Periplaneta americana Extract on Alopecia Areata Mice through Alleviating Skin Inflammation and Modulating Skin Microbiota Based on Systems Pharmacology Mendeley Data Alopecia areata (AA), an autoimmune hair-loss disease due to follicular inflammatory cell infiltration, lacks an ideal cure. the Periplaneta Americana (PA) extract PA-011, a traditional Chinese medicine with anti-inflammatory, antioxidant, tissue-repair-promoting, and immune-regulating properties, was studied for its AA-treating effects. Guided by network pharmacology, AA model mice were treated with PA-011. Hair growth, skin tissue, inflammatory factors, and Wnt protein levels were examined. Transcriptomics, metabolomics, and 16S rRNA sequencing explored the hair-growth-promoting mechanisms. Network pharmacology showed PA-011 could regulate AA-related targets and pathways. PA-011 intervention promoted hair follicle cell proliferation and hair growth in AA mice, reduced skin TNF-α, IL-23, and VCAM-1 expression. Transcriptomics and WB analysis indicated PA-011 downregulated inflammatory genes, activated Wnt3a, and modulated the TGF-β pathway. Metabolomics found PA-011 regulated metabolic pathways. 16S rRNA analysis showed it reversed AA-induced skin microbiota changes, inhibiting pathogens and promoting probiotics. Multi-omics analysis revealed PA-011 regulated skin microbiota and metabolic balance for hair growth. In conclusion, PA-011 alleviates AA by reducing skin inflammation and modulating skin microbiota, suggesting its potential as an AA therapeutic. Animal Pharmacology Dataset doi:10.17632/7mncdjvwpb.1 oai:data.mendeley.com/7mncdjvwpb.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/7mncdjvwpb 2025-02-20T18:36:10Z