2026-10-15T17:58:33Z https://data.mendeley.com/oai

oai:data.mendeley.com/7pn4pwj3td.1 2025-04-02T09:49:03Z

Ferraro, Mariola Gut Microbiome Profiles in CB2R-Knockout and Wild-Type Mice With and Without Salmonella Infection Mendeley Data This dataset examines how genetic deletion of cannabinoid receptor 2 (CB2R) impacts gut microbiome composition during Salmonella enterica serovar Typhimurium infection. CB2R is a G protein-coupled receptor involved in regulating immune responses and intestinal homeostasis. Its expression in immune cells and the gastrointestinal tract suggests a potential role in shaping host-microbiota interactions. To investigate whether CB2R deficiency alters microbial dynamics in a way that influences susceptibility to infection, we compared CB2R knockout (CB2R-KO) mice with wild-type (WT) littermate controls in an oral Salmonella challenge model. Fecal samples were collected at two time points: pre-infection (baseline) and four days post-infection. Samples were subjected to shallow shotgun metagenomic sequencing (1×150 bp, Illumina). Raw reads were filtered using Vsearch to remove low-quality sequences and short fragments. Taxonomic classification was performed with Kraken2, and processed data were analyzed using R (phyloseq, ALDEx2, microbiome packages). Alpha diversity analysis showed no significant difference in species richness or evenness between genotypes. However, CB2R-KO mice exhibited distinct shifts in microbial composition following infection. These findings suggest that CB2R contributes to maintaining a balanced gut microbial ecosystem and that its absence may promote dysbiosis during enteric infection, potentially increasing host susceptibility. Gut Microbiome Barker, Hailey Roesch, Luiz Dataset doi:10.17632/7pn4pwj3td.1 oai:data.mendeley.com/7pn4pwj3td.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/7pn4pwj3td 2025-04-02T09:49:03Z