2026-10-11T22:42:38Z https://data.mendeley.com/oai

oai:data.mendeley.com/7t9snvwv3p.1 2025-01-22T17:50:04Z

Xing, Lixiao Early fusion intermediate of ACE2-using coronavirus spike acting as antiviral target Mendeley Data Coronavirus fusion with and entry into the host cell depends on viral spike, which acts as a crucial component of viral infection. However, the lack of receptor-activated spike intermediate conformation has hindered a comprehensive understanding of spike-induced membrane fusion. Here, we captured an ACE2-induced early fusion intermediate conformation (E-FIC) of SARS-CoV-2 spike in which HR1 in S2 has ejected while S1 remains attached. This E-FIC can transition to the late fusion intermediate conformation after S2' cleavage. Leveraging this discovery, we designed an E-FIC-targeted dual-functional antiviral protein AL5E. AL5E effectively inactivated ACE2-using coronaviruses and inhibited their infection, outperforming a mono-functional antiviral in protecting animals against these coronaviruses. This study has identified the E-FIC and used it as target for the development of a dual-functional antiviral for the prevention and treatment of ACE2-using coronavirus infection. Membrane Fusion Viral Entry Antiviral Agent Active against Respiratory Viruses Coronavirus Liu, Zhimin Wang, Xinling Liu, Qianying Xu, Wei Mao, Qiyu Zhang, Xiang Hao, Aihua Xia, Shuai Liu, Zezhong Sun, Lujia Zhang, Guangxu Wang, Qian Chen, Zhenguo Jiang, Shibo Sun, Lei Lu, Lu Dataset doi:10.17632/7t9snvwv3p.1 oai:data.mendeley.com/7t9snvwv3p.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/7t9snvwv3p 2025-01-22T17:50:04Z