2026-10-14T05:49:06Z https://data.mendeley.com/oai

oai:data.mendeley.com/8j3rcgs7jv.1 2024-03-25T05:11:47Z

Wehr, Michael Protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay Mendeley Data The ERBBprofiler assay measures compound effects on ERBB family receptors and key downstream signaling pathways that are implicated in cancer or other complex diseases. Here, we present a protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay. We describe steps for in solution transfection, cell treatment, combined processing of samples, amplification and indexing of PCRs, sequencing, and data analysis. This approach allows for the simultaneous assessment of compound effects and cell type dependent effects. For complete details on the use and execution of this protocol, please refer to Popović et al, 2024, iScience. Epidermal Growth Factor Receptor Drug Screening Cell-Based Assays Popovic, Luksa Rossner, Moritz Dataset doi:10.17632/8j3rcgs7jv.1 oai:data.mendeley.com/8j3rcgs7jv.1 Attribution-NonCommercial 3.0 Unported https://creativecommons.org/licenses/by-nc/3.0 https://data.mendeley.com/datasets/8j3rcgs7jv 2024-03-25T05:11:47Z