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oai:data.mendeley.com/8n5h7v2965.1 2026-06-23T21:31:24Z

Jian, Xing Acute Slow Oscillation Power After Photothrombotic Stroke Wide-Field Calcium Imaging, Behavioral, and Histological Dataset Digital Commons Data@Becker This dataset contains multimodal neuroscience data collected from 25 adult Thy1-GCaMP transgenic mice (~8–12 months of age) undergoing photothrombotic (PT) stroke, generated to assess functional and behavioral changes at acute and chronic timepoints following ischemic injury. Data were acquired from a single biological source — live mice — with tissue samples subsequently processed for post-mortem histological analysis. Three data modalities are included: widefield calcium imaging (WFCI) capturing cortex-wide neural activity via the genetically encoded calcium indicator GCaMP; behavioral data from the cylinder rearing test, which quantifies forelimb asymmetry as a measure of sensorimotor deficit; and histological data comprising both Cresyl Violet (Nissl) staining for lesion visualization and immunohistochemistry (IHC) for protein-specific labeling. Each mouse was assessed at three experimental timepoints: baseline (pre-stroke), acute (24 hours post-PT stroke), and chronic (one week post-PT stroke), with histology collected only at the post-chronic endpoint. The dataset is hierarchically organized under a top-level /data directory containing three primary modality folders: /behavior, /WFCI, and /histology. The /behavior folder is organized by mouse ID (e.g., /M01) and then by timepoint (/bsl, /acute, /weekone), containing raw video recordings, Excel-format scoring sheets, and aggregated GraphPad Prism files summarizing results across all animals. The /WFCI folder is subdivided into three processing tiers — /Raw (unprocessed imaging files), /Tier1_Derived (intermediate processed data), and /Tier2_SourceData (analysis-ready outputs organized by mouse and timepoint) — supporting reproducibility from raw acquisition through final analysis. The /histology folder contains two subfolders, /cresyl_violet and /IHC, holding stained tissue section images. File formats include TIFF images for histology, CSV and XLSX for behavioral scoring, and standard video formats for raw behavior recordings. The organizational rationale — mouse ID → modality → timepoint — enables straightforward longitudinal comparisons within subjects and cross-sectional comparisons across the cohort. Data were generated by Jake Lee and Arnav Ajay Jadav in the Landsness Lab, Department of Neurology, Washington University in St. Louis (contact: jake.l@wustl.edu), and are suitable for analyses of stroke-induced changes in cortical dynamics, motor behavior, and structural pathology. Neuroscience Pathology Motor Behavior Lee, Jake Jadav, Arnav A. Landsness, Eric Dataset doi:10.17632/8n5h7v2965.1 oai:data.mendeley.com/8n5h7v2965.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://digitalcommonsdata.wustl.edu/datasets/8n5h7v2965 2026-06-23T21:31:24Z