2026-10-12T19:07:06Z https://data.mendeley.com/oai

oai:data.mendeley.com/8pkk8y3xht.1 2025-04-11T07:14:43Z

Jogi, Shraddha Comparative Transcriptome Analysis of Wild-Type and Metabolically Engineered Pichia pastoris (Komagataella phaffii) for β-Lactam Precursor δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine (ACV) Production Mendeley Data This dataset "Comparative Transcriptome Analysis of Wild-Type and Metabolically Engineered Pichia pastoris (Komagataella phaffii) for β-Lactam Precursor ACV Production" supports the manuscript titled “Metabolic Engineering of Pichia pastoris for Production of the β-Lactam Precursor δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine (ACV)”. It includes raw transcriptomic data (RNA-Seq FASTQ files) and the results of Differential Gene Expression (DEG) analysis comparing a wild-type strain of Pichia pastoris (also known as Komagataella phaffii) with a genome engineered strain designed to produce the β-Lactam precursor δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine (ACV). Two biological replicates were analyzed via RNA-Seq for each condition (wild-type and engineered). The dataset contains: 1. Raw sequencing reads (FASTQ]). 2. Processed DEG analysis results (tables in CSV format) including gene identifiers, log2 fold changes, p-values, and adjusted p-values (FDR). Pichia pastoris is a widely used host for heterologous protein and metabolite production. This transcriptomic data provides insights into the global gene expression changes resulting from the metabolic engineering strategies employed for ACV synthesis. Synthetic Biology Metabolic Engineering Raghunathan, Anu Dataset doi:10.17632/8pkk8y3xht.1 oai:data.mendeley.com/8pkk8y3xht.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/8pkk8y3xht 2025-04-11T07:14:43Z