2026-10-15T08:26:47Z https://data.mendeley.com/oai

oai:data.mendeley.com/bfhtr3gtbg.2 2026-10-13T18:09:30Z

xu, xiaofan Enhanced Accumulation of High-Value Acylated Anthocyanins and Improved Growth in Lemna turionifera with SpANS Overexpression Mendeley Data Anthocyanins are valuable natural pigments but their large-scale production is limited by inefficient plant extraction and low microbial productivity. Spirodela polyrhiza accumulates abundant anthocyanins but exhibits limited suitability for industrial production, whereas Lemna turionifera shows strong environmental adaptability and efficient genetic transformation capacity. Here, six L. turionifera lines overexpressing SpANS were generated. These lines showed marked phenotypic changes and up to a tenfold increase in anthocyanin accumulation relative to the wild type. RNA-seq indicated that SpANS overexpression may activate MAPK signaling and coordinate hormone signaling, photosynthesis, and carbon and nitrogen metabolism, thereby promoting both growth and secondary metabolism. Targeted metabolomics revealed that SpANS overexpression enhanced anthocyanin accumulation in L. turionifera, particularly promoting the biosynthesis of high-value acylated anthocyanins. Collectively, this study establishes engineered L. turionifera as a promising plant-based biomanufacturing platform for sustainable production of valuable anthocyanin derivatives and provides new insights into the development of duckweed as an industrial crop for natural product biosynthesis. Duckweed Dataset doi:10.17632/bfhtr3gtbg.2 oai:data.mendeley.com/bfhtr3gtbg.2 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/bfhtr3gtbg 2026-10-13T18:09:30Z