2026-10-15T08:26:08Z https://data.mendeley.com/oai

oai:data.mendeley.com/czzb4jb8ss.1 2023-02-03T13:03:24Z

li, wenbo SARS-CoV-2 restructures host chromatin architecture Mendeley Data Some viruses restructure host chromatin, influencing gene expression, with implications for disease outcome. Whether this occurs for SARS-CoV-2, the virus causing COVID-19, is largely unknown. Here, we characterized the 3D genome and epigenome of human cells after SARS-CoV-2 infection, finding widespread host chromatin restructuring that features widespread A compartmental weakening, A-B mixing, reduced intra-TAD contacts and decreased H3K27ac euchromatin modification levels. Such changes were not found following common-cold virus HCoV-OC43 infection. Intriguingly, the cohesin complex was significantly depleted from intra-TAD regions, indicating that SARS-CoV-2 disrupts cohesin loop extrusion. These altered 3D genome/epigenome structures correlated with transcriptional suppression of interferon response genes by the virus, while increased H3K4me3 was found in the promoters of pro-inflammatory genes highly induced during severe COVID-19. These findings show that SARS-CoV-2 acutely rewires host chromatin, facilitating future studies of the long-term epigenomic impacts of its infection. This work is to be published in Nature microbiology (Wang et al., 2023). Confocal Microscopy Dataset doi:10.17632/czzb4jb8ss.1 oai:data.mendeley.com/czzb4jb8ss.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/czzb4jb8ss 2023-02-03T13:03:24Z