2026-10-12T01:19:50Z https://data.mendeley.com/oai

oai:data.mendeley.com/d5mg48z55p.1 2022-06-17T20:58:16Z

Dubé, Mathieu Temporal associations of B and T cell immunity with robust vaccine responsiveness in a 16-week interval BNT162b2 regimen. Nayrac et al. Mendeley Data Spacing of the BNT162b2 mRNA doses beyond 3 weeks raised concerns about vaccine efficacy. We longitudinally analyzed B cell, T cell and humoral responses to two BNT162b2 mRNA doses administered 16 weeks apart in 53 SARS-CoV-2 naïve and previously-infected donors. This regimen elicited robust RBD-specific B cell responses whose kinetics differed between cohorts, the second dose leading to increased magnitude in naïve participants only. While boosting did not increase magnitude of CD4+ T cell responses further compared to the first dose, unsupervised clustering of single-cell features revealed phenotypic and functional shifts over time and between cohorts. Integrated analysis showed longitudinal immune component-specific associations, with early Thelper responses post-first dose correlating with B cell responses after the second dose, and memory Thelper generated between doses correlating with CD8 T cell responses after boosting. Therefore, boosting elicits a robust cellular recall response after the 16-week interval, indicating functional immune memory. Statistics Nayrac, Manon Sannier, Gérémy Nicolas, Alexandre Marchitto, Lorie Tastet, Olivier Tauzin, Alexandra Brassard, Nathalie Lima-Barbosa, Raphaël Beaudoin-Bussières, Guillaume Vezina, Danie Yu Gong, Shang Ben, Med Gasser, Romain Laumaea, Annemarie Prévost, Jérémie Bourassa, Catherine Gendron-Lepage, Gabrielle Medjahed, Halima Goyette, Guillaume Ortega-Delgado, Gloria Gabrielle Laporte, Mélanie Niessl, Julia Gokool, Laurie Morrisseau, Chantal Arlotto, Pascale Richard , Jonathan Bélair, Justin Prat, Alexandre Tremblay, Cécile Laferrière, Valérie Finzi, Andrés Kaufmann, Daniel Dataset doi:10.17632/d5mg48z55p.1 oai:data.mendeley.com/d5mg48z55p.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/d5mg48z55p 2022-06-17T20:58:16Z