2026-10-15T10:19:41Z https://data.mendeley.com/oai

oai:data.mendeley.com/dzxcw45vp6.1 2025-04-07T09:45:15Z

Feeney, Austin Enhancing human pluripotent stem cell differentiation to cardiomyocytes through cardiac progenitor reseeding and cryopreservation Mendeley Data Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have the potential to transform understanding of heart development and treatment of heart failure. However, hPSC-CM differentiation efficiency is plagued by batch-to-batch and line-to-line variability. Here, we describe a method to improve CM purity by 10-20% (absolute) without negatively affecting contractility, sarcomere structure, multinucleation, junctional Cx43, or CM number by detaching and reseeding progenitors between the EOMES+ mesoderm and ISL1+/NKX2-5+ cardiac progenitor stages. Moreover, we demonstrate that EOMES+ mesoderm and ISL1+/NKX2-5+ cardiac progenitors are cryopreservable with similar improvements in CM purity after resuming differentiation, facilitating storage of large batches of hPSC-CM progenitors for on-demand CM production. Reseeding during differentiation also enables transition to defined extracellular matrices including fibronectin, vitronectin, and laminin-111, which all supported hPSC-derived EOMES+ mesoderm and ISL1+/NKX2-5+ cardiac progenitor differentiation to CMs. In summary, we present a method to increase hPSC-CM differentiation purity and demonstrate that specific CM progenitors are amenable to cryopreservation. Cell Differentiation Simmons, Aaron Peplinski, Claire Zhang, Xiaotian Palecek, Sean Dataset doi:10.17632/dzxcw45vp6.1 oai:data.mendeley.com/dzxcw45vp6.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/dzxcw45vp6 2025-04-07T09:45:15Z