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Beckman, Danielle SARS-CoV-2 infects neurons and induces neuroinflammation in a non-human primate model of COVID-19. Beckman et al. 2022 Mendeley Data A supplementary file containing the videos associated with the study by Beckman et al., 2022. Movie 01 (00:00) – 3D representation of SARS-CoV-2 proteins (dsRNA – red, SARS-CoV-2-Spk – purple) within a neuron (MAP2 – green, DAPI - blue) located in the entorhinal cortex (ERC) of an aged, infected animal. dsRNA is found within the soma, closely associated with its degrading nucleus, while SARS-CoV-2-Spk is found distributed throughout the surface of the soma and MAP2+ processes; Movie 02 (00:45) - 3D representation of SARS-CoV-2 proteins (dsRNA – red, SARS-CoV-2-Spk – purple) within a neuron (MAP2 – green, DAPI - blue) located in the ERC of an aged, infected animal. The presence of SARS-CoV-2 within a neuron is associated with suppression of MAP2 immunosignal, suggesting infection is associated with decreased neuronal integrity. Movie 03 (01:30) - 3D representation of SARS-CoV-2 proteins (dsRNA – red, SARS-CoV-2-Spk – purple) within neurons (MAP2 – green, DAPI - blue) located in the piriform cortex of an aged, infected animal. Different degrees of dsRNA immunosignal can be found in neurons, suggesting a progressive replication process following SARS-CoV-2 infection accompanied by neuronal degeneration; Movie 04 (02:10) - 3D representation of astrocytes (GFAP – white) and microglia (IBA1 – green) contacting neurons (NeuN – blue) positive for nucleocapsid protein (SARS-CoV-2-N – red) in the olfactory cortex of a young, infected animal. The directed contact between infected neurons and glial cells suggests that infection triggers a neuroinflammatory response that may contribute to neuronal death; Movie 05 (02:55) - 3D representation of microglia (IBA1 – red, DAPI - blue) interacting with neurons (Pan Neurofilament – white, DAPI – blue) and dendritic spines (PSD95 – green) in the olfactory cortex of an infected animal. The contact between microglia and neurons suggests a direct action of microglia over neuronal degeneration, a mechanism underscored by the substantial engulfment of PSD95+ puncta; Movie 06 (03:35) - 3D of microglia (IBA1 – red) interacting with neurons (Pan Neurofilament – white) and dendritic spines (PSD95 – green) in the piriform cortex of an infected animal, indicating extensive dendritic spine engulfment by activated microglia in SARS-CoV-2-bearing areas; Movie 07 (04:10) - 3D representation of an activated neutrophil (Myeloperoxidase – red, DAPI - blue) recruitment associated with blood-brain barrier disruption, as evidenced by disorganization of vascular astrocytic end feet (GFAP – green), among neurons (NeuN – white, DAPI – blue) of the primary olfactory cortex of a SARS-CoV-2 infected animal; Movie 08 (04:47) - 3D representation of an activated neutrophil (Myeloperoxidase – white, DAPI - blue) associated with blood-brain barrier disruption, as evidenced by disorganization of vascular astrocytic end feet (GFAP – red) among neurons (NeuN – green, DAPI – blue) of the olfactory cortex of a SARS-CoV-2 infected animal. Neuroscience Neuro-Inflammation Severe Acute Respiratory Syndrome Coronavirus 2 Dataset doi:10.17632/j4wdhwh3sw.1 oai:data.mendeley.com/j4wdhwh3sw.1 Attribution-NonCommercial 3.0 Unported https://creativecommons.org/licenses/by-nc/3.0 https://data.mendeley.com/datasets/j4wdhwh3sw 2022-10-06T07:30:01Z