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            <header>
                <identifier>oai:data.mendeley.com/rr9t3ccbc5.1</identifier>
                <datestamp>2025-07-21T09:16:01Z</datestamp>
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            <metadata><oai_dc:dc xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
    <dc:creator>Frail, Sarah</dc:creator>
    <dc:title>Data for Genomes of nitrogen-fixing eukaryotes reveal an alternate path for organellogenesis</dc:title>
    <dc:publisher>Mendeley Data</dc:publisher>
    <dc:description>Raw data files used in the analyses for the in-prep 2024 manuscript Genomes of nitrogen-fixing eukaryotes reveal a non-canonical model of organellogenesis . Summary below

File descriptions:

Genome files:
Ep = E. pelagica, Ec = E. clementina. E. pelagica genome assembly is previously published and available at GCA_946965045.2, all E. pelagica files are compatible with the NCBI files. The diazoplast of E. clementina was previously published at GCA_029919255.1
[diatom].gtf    de novo annotation feature file
[diatom].faa    translation of annotation feature file
Ec.nuc.fasta    nuclear genome of E. clementina
Ec. mito.fasta    mitochondrial genome of E. clementina
Ec.chlo.fasta    chloroplast genome of E. clementina

Figure 1: 
1A_SpeciesAccessions.xlsx     All accessions for sequences
1C_[diatom].masked.comparative.summary.tbl    Output tables form the denovo repeatmasker run. 
1D_Pairwise percent identity.csv    All pairwise % amino acid identity values of protein orthologues between species pairs
1E_DiatomOrthogroups.xlsx    Orthogroups and genes within them (orthofinder Orthogroups.tsv file)
1E_Orthogroup_overlap_and_percent_identity_statistics.csv   Pairwise % identity of protein orthologues and jaccard index of the overlap between orthogroups
1E_Individual_orthogroup_membership.csv    Orthogroups and number of genes for each species contained within them (orthofinder Orthogroups.GeneCount.tsv file)
1E_HGTRawResults.xlsx    Output of HGT search for all diatoms. The result fields include, HGT confidence based on tree topology, AI score, and top blast hit taxa
1E_Ec_HGT_trees.tar.gz    Raw phylogenetic trees used to identify HGTs and summary of HGT calls for E. clementina
1E_Ep_HGT_trees.tar.gz    As above, for E. pelagica
1E_StudyGeneCodesID.xlsx  Table associating the simple gene codes used in the orthogroup analyses in this study to their full corresponding gene IDs on NCBI and other databases

Figure 2:
2A_Ec.masked.regions.gff    Repeat regions used for genome tracks

Figure 4:
4B_4C_ProcessedProteomicsData.xlsx    Processed and unprocessed proteomics tables
4B_4C_20230228_orbitrapqexactive_lysate.raw    The raw proteomics data from whole cell lysate used for analysis in Figure 5B and C. Run on the orbitrap q-exactive
4B_4C_20231030_orbitrapeclipse_lysate.raw    for whole cell lyste, run on the orbitrap eclipse
4B_4C_20230421_orbitrapeclipse_lysate.raw    for whole cell lyste, run on the orbitrap eclipse
4B_4C_20231030_orbitrapeclipse_diazo.raw    for diazoplast fraction, run on the orbitrap eclipse
4B_4C_20230421_orbitrapeclipse_diazo.raw    for diazoplast fraction, run on the orbitrap eclipse
4B_4C_20230228_orbitrapqexactive_diazo.raw    for diazoplast fraction, run on the orbitrap q-exactive

</dc:description>
    <dc:subject>Genomics</dc:subject>
    <dc:subject>Proteomics</dc:subject>
    <dc:subject>Horizontal Gene Transfer</dc:subject>
    <dc:subject>Evolution Symbiosis</dc:subject>
    <dc:type>Dataset</dc:type>
    <dc:identifier>doi:10.17632/rr9t3ccbc5.1</dc:identifier>
    <dc:identifier>oai:data.mendeley.com/rr9t3ccbc5.1</dc:identifier>
    <dc:rights>Creative Commons Attribution 4.0 International</dc:rights>
    <dc:rights>http://creativecommons.org/licenses/by/4.0</dc:rights>
    <dc:relation>https://data.mendeley.com/datasets/rr9t3ccbc5</dc:relation>
    <dc:date>2025-07-21T09:16:01Z</dc:date>
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