2026-10-15T09:16:45Z https://data.mendeley.com/oai

oai:data.mendeley.com/sjnkwyjw68.1 2026-10-09T13:58:49Z

Winkler, Wade Tran Peterson et al The core interaction network of transcription elongation factors is broadly maintained across diverse bacteria Mendeley Data Molecular mechanisms of bacterial transcription elongation have been investigated most thoroughly for the model bacterium Escherichia coli. E. coli elongation factor NusG associates through its N-terminal domain (NTD) with the transcription elongation complex (TEC), while its C-terminal domain (CTD) forms mutually exclusive interactions with either ribosomal protein S10 or Rho termination factor. The E. coli NusG:S10 complex creates a bridge that directly couples the TEC with a pioneering ribosome. However, the E. coli NusG:S10 complex also participates in antitermination complexes (ATC) that inhibit Rho termination and increase transcription of rRNA operons. A characteristic sequence in the nascent rrn RNA, called boxA, loads E. coli NusB:S10 onto the NusG-bound TEC, leading to formation of the rrn ATC. However, these findings may not extend to all bacteria. The role of Rho-dependent termination appears to be less important in many Gram-positive bacteria, including some that do not encode for Rho. Moreover, transcription and translation remain uncoupled within Bacillus subtilis and many other Gram-positive bacteria. Therefore, it is unclear whether the interaction network for E. coli elongation factors also occurs in B. subtilis and other Gram-positive bacteria. To investigate this, we identified boxA-like sequences upstream of 16S and 23S genes in Bacillus species and found they associate directly with NusB. We also found that B. subtilis S10 associates directly with both NusB and NusG, and that Rho can also associate with NusG. Together, these data demonstrate that B. subtilis elongation factors form an interaction network similar to E. coli, which in theory could participate in transcription-translation coupling or formation of the rrn ATC. Transcription Elongation Bacillus subtilis Bacterial Transcription Dataset doi:10.17632/sjnkwyjw68.1 oai:data.mendeley.com/sjnkwyjw68.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/sjnkwyjw68 2026-10-09T13:58:49Z