2026-10-10T09:43:59Z https://data.mendeley.com/oai

oai:data.mendeley.com/trvt5fxyrm.1 2024-06-11T19:06:46Z

刘, 刘洁 Supplementary material of Phage-based magnetic capture method as an aid for real-time recombinase polymerase amplification detection of Salmonella spp. in milk Mendeley Data Salmonella is a major cause of foodborne diseases worldwide. Conventional rapid assays for detecting Salmonella in real samples often encounter severe matrix interference or detect the limited number of species of a genus, resulting the inaccuracy of detection. In this study, we developed a method that combined phage-based magnetic capture with real time recombinase polymerase amplification (RPA) for the rapid, highly sensitive, and specific detection of Salmonella in milk with an ultra-low detection limit. The Felix O-1 phage-conjugated magnetic beads (O-1 pMBs) synthesized in this method showed excellent capture ability for Salmonella spp. and ideal specificity for non-Salmonella strains. After O-1 pMBs-based magnetic separation, the limit of detection (LOD) of the real time RPA assay was 50 CFU/mL in milk samples, which was significantly increased by a magnitude of 3–4 orders. The method exhibited a high sensitivity (compatibility) of 100% (14/14) for all tested Salmonella serotype strains and an ideal specificity (exclusivity) of 100% (7/7) for the tested non-Salmonella strains. The entire detection process including Salmonella capture, DNA extraction, and real time RPA detection was completed within 1.5 h. Furthermore, milk samples spiked with 10 CFU/25 mL of Salmonella were detected positive after cultured in buffered peptone water for only 3 h. Therefore, the proposed method could be an alternative for the rapid and accurate detection of Salmonella. Advanced Material Dataset doi:10.17632/trvt5fxyrm.1 oai:data.mendeley.com/trvt5fxyrm.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/trvt5fxyrm 2024-06-11T19:06:46Z