2026-10-12T00:25:40Z https://data.mendeley.com/oai

oai:data.mendeley.com/w24t4trkv5.2 2025-01-06T16:44:39Z

Sato, Yohei A dataset on NFKB1/NFKB2 knockout Jurkat cells and primary CD4+ T cells generated by CRISPR/Cas9 mediated gene disruption. Mendeley Data Specific biological roles of non-canonical nuclear factor-kappa B (NFKB) signaling in human T cells remain unclear. Therefore, we genetically modified Jurkat leukemic cells using the clustered regularly interspaced palindromic repeat (CRISPR)/CRISPR-associated protein 9 technology to better understand the NFKB signaling mechanisms. IKAROS family zinc finger-2 levels were downregulated in NFKB2-knocked-out cells but upregulated in NFKB1-knocked-out cells. To further understand the biological roles of NFKB signaling in CD4+ T cells, NFKB1/NFKB2 loci of human primary CD4+ T cells were genetically edited using the CRISPR/CRISPR-associated protein 9 technology. Quantitative polymerase chain reaction and fluorescence-activated cell sorting revealed the similar phenotypes and gene expression profiles of NFKB1/NFKB2-knocked-out human CD4+ T cells. Immunology Biotechnology Genome Editing Dataset doi:10.17632/w24t4trkv5.2 oai:data.mendeley.com/w24t4trkv5.2 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/w24t4trkv5 2025-01-06T16:44:39Z