2026-10-15T21:08:32Z https://data.mendeley.com/oai

oai:data.mendeley.com/wdsystvjng.1 2021-09-15T21:30:43Z

wang, jian An antibody-based proximity labelling map reveals mechanisms of SARS-CoV-2 inhibition of antiviral immunity. Zhang et al. Mendeley Data The global epidemic caused by the coronavirus SARS-CoV-2 has resulted in the infection of over 200 million people. To extend the knowledge of interactions between SARS-CoV-2 and humans, we systematically investigate the interactome of 29 viral proteins in human cells by using an antibody-based TurboID assay. In total, 1388 high-confidence human proximal proteins with biotinylated sites are identified. Notably, we find that SARS-CoV-2 manipulates the antiviral and immune responses. We validate that the membrane protein ITGB1 associates ACE2 to mediate SARS-CoV-2 entry. Moreover, we reveal that SARS-CoV-2 proteins inhibit activation of the interferon pathway through the mitochondrial protein MAVS and the methyltransferase SETD2. We propose 111 potential drugs for the clinical treatment of COVID-19 and identify three compounds that significantly inhibit the replication of SARS-CoV-2. The proximity labelling map of SARS-CoV-2 and humans provides a resource for elucidating the mechanisms of viral infection and developing drugs for COVID-19 treatment. Medicine Life Sciences Interactome Interaction Proteomics Dataset doi:10.17632/wdsystvjng.1 oai:data.mendeley.com/wdsystvjng.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/wdsystvjng 2021-09-15T21:30:43Z