2026-10-16T01:28:23Z https://data.mendeley.com/oai

oai:data.mendeley.com/wynyf35xf3.2 2021-10-13T14:26:44Z

Sarabia-Vega, Vanessa A low-cost and open-source protocol to produce key enzymes for molecular detection assays. Mendoza-Rojas et al. (2021) Mendeley Data Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes for molecular detection of pathogens. Based on previous reports, we optimized the protocol for LbCas12a, Taq DNA polymerase, M-MLV reverse transcriptase, and TEV protease to make it compatible with minimal laboratory equipment, broadly available in low- and middle-income countries. The enzymes produced with this protocol have been successfully used for molecular detection applications. For complete details on the use of this protocol, please refer to Alcantara et al. (2021). Biochemistry Molecular Biology COVID-19 Mendoza-Rojas, Gabriel Sanchez-Castro, Ana Tello, Lesia Cabrera-Sosa, Luis Nakamoto, Jose A. Peñaranda, Katherin Adaui, Vanessa Alcántara, Roberto Milón, Pohl Dataset doi:10.17632/wynyf35xf3.2 oai:data.mendeley.com/wynyf35xf3.2 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/wynyf35xf3 2021-10-13T14:26:44Z