2026-10-12T12:45:30Z https://data.mendeley.com/oai

oai:data.mendeley.com/x3vkrx9hcm.1 2018-08-30T09:23:57Z

Kuang, Haixue Data for: Comparisons of the pharmacokinetic and tissue distribution profilesof withanolide B after intragastric administration of the effective part of Datura metel L. in normal and psoriasis guinea pigs Mendeley Data A simple, highly sensitive ultra-performance liquid chromatography- electrospray ionization-mass spectrometry (LC-ESI-MS) method has been developed to quantify of withanolide B and obakunone (IS) in guinea pig plasma and tissues, and to compare the pharmacokinetics and tissue distribution of withanolide B in normal and psoriasis guinea pigs. After mixing with IS, plasma and tissues were pretreated by protein precipitation with methanol. Chromatographic separation was performed on a C18 column using aqueous (0.1% formic acid) and acetonitrile (0.1% formic acid) solutions at 0.4 mL/min as the mobile phase. The gradient program was selected. Detection was performed on a 4000 QTRAP UPLC–ESI-MS/MS system from AB Sciex in the multiple reaction monitoring (MRM) mode. Withanolide B and obakunone (IS) were monitored under positive ionization conditions. The optimized mass transition ion-pairs (m/z) for quantitation were 455.1/109.4 for withanolide B and 455.1/161.1 for obakunone. Liquid Chromatography Zhang, Lijie Yang, Lianrong Meng, Xin Yang, Bingyou Dataset doi:10.17632/x3vkrx9hcm.1 oai:data.mendeley.com/x3vkrx9hcm.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/x3vkrx9hcm 2018-08-30T09:23:57Z