2026-10-15T09:32:18Z https://data.mendeley.com/oai

oai:data.mendeley.com/z7jjchsfts.1 2026-09-01T12:59:58Z

Haraźna, Katarzyna Enzyme-Mediated Crosslinking of Silk Fibroin/ N-Acetylcysteine-Modified Chitosan/ Hydroxyapatite Hydrogel Composites for Subchondral Bone Regeneration - additional experiments Mendeley Data This dataset contains the results of comprehensive physicochemical, morphological, structural, and biological analyses of hydrogel composites based on silk fibroin, N-acetylcysteine-modified chitosan, and hydroxyapatite. The investigated materials were prepared using an enzymatic cross-linking approach and evaluated as composite hydrogel systems for potential application in subchondral bone regeneration. The data include the results of additional experiments conducted during the peer-review process and comprise: (1) direct biocompatibility assessment, including absorbance measurements and fluorescence microscopy observations; (2) alkaline phosphatase (ALP) activity measurements, together with total protein quantification at the corresponding time points; (3) Alizarin Red S mineralization assays, including absorbance measurements and microscopic observations; (4) ELISA measurements of osteocalcin (OCN) and RUNX2, normalized to total protein content determined using the BCA assay; (5) rheological measurements; (6) scanning electron microscopy (SEM) observations; and (7) assessment of superoxide anion (O₂•⁻) generation in THP-1 cells using the nitroblue tetrazolium (NBT) assay. This research was funded by the National Science Centre, Poland, under the project “Hierarchical approaches to osteochondral tissue engineering” (grant no. UMO-2022/45/B/ST8/02557). Polymer Biopolymer Ceramic Composite Hydrogel Enzyme Catalysis Polysaccharide Scaffolds for Bone Regeneration Bone Regeneration Träger, Dominika Winiarska, Agnieszka Wesołowska, Julita Niziołek, Karina Slota, Dagmara Lis, Kamila Sobczak-Kupiec, Agnieszka Dataset doi:10.17632/z7jjchsfts.1 oai:data.mendeley.com/z7jjchsfts.1 Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 https://data.mendeley.com/datasets/z7jjchsfts 2026-09-01T12:59:58Z