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  • This thesis describes the study of mutant mice which harbour congenital defects in various aspects of erythropoiesis, obtained through ENU mutagenesis. Mice were phenotyped and characterised in order to understand the underlying genetic and molecular mechanisms which resulted in their red cell defects, as well as their clinical relevance to human red cell diseases.
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  • Post-conflict societies and transitional states, including those in our region the Pacific, are increasingly seen as fertile ground for the imposition of externally designed legal systems. Imposition can occur as a result of NGO advocacy, the transposition of 'ready made' legal systems by the international civil society or by supra-national and international organisations. Not only is the existence of local customary legal system.s often ignored during this process, but the strengths and inherent infrastructure of customary systems are often not capitalised on during the implementation phase of the new system, ultimately to the detriment of the state's legal system. The result of these,failures is that establishment of law and order proves more elusive than it otherwise could be. Customary and traditional legal systems, in their many varied,fbrms, continue to provide a source of social stability and a basis on which indigenous legal development could occur in numerous post-conflict and transitional states around the world. Additionally, customary law al.so,functions as a central element of peoples' cultural integrity and heritage. By using transitional South Sudan as a research case study, important lessons are brought to the fore about the advantages and importance of both acknowledging and building on existing local legal systems in transitional states. Customary legal systems can potentially provide a key avenue through which to expedite rule of law and judicial development in post-conflict and transitional states. Insights into this process are provided by an examination ofthe interfaces in South Sudan between local customary legal systems, nascent statutory regimes und internationally promoted human rights standards.
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  • This thesis explores young rural adults’ psychosocial recovery beyond the 2009 Gippsland bushfires and addresses the paucity of research on young adults’ recovery from this disaster. Because of climate change, scientists predict such events are likely to increase in frequency and severity. Climate change has the potential to profoundly affect the Gippsland region’s social, environmental, economic, and health outcomes, and recovery needs into the future.
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  • This performance-based research correlates three fundamental compositional parameters of Romanian composer Livia Teodorescu-Ciocanea’s work – timbre, texture and structure – with a set of performance guidelines resulting from Smolyar’s pianistic experience. Smolyar has intensively examined the timbral nuances that the pianoforte is able to achieve through an examination of the underlying structure and texture of Teodorescu-Ciocănea’s music, which is structured along spectralist lines and the composer’s innovative concept of ‘hypertimbralism’. The research aims to identify the role of timbre in the creation of musical meaning for each performance situation via score analysis and interpretative approaches depending on the physical requirements of playing the piano.
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  • The disulfide-dithioloxidoreductase enzyme DsbA is an oxidative folding catalyst found in the bacterial periplasm and is a key determinant of virulence in Gram-negative pathogens. DsbA has a wide spectrum of substrate specificity and has been demonstrated to catalyse the formation of disulfides in numerous secreted proteins. DsbA operates at a central point in the production of virulence-determinants because most virulence factors are proteins that require disulfide bonds to be active. DsbA knockout mutants in E. coli, have been shown to be impaired in a range of processes related to virulence. Significantly, DsbA mutations in many pathogenic bacteria attenuate their virulence, demonstrating the value of targeting DsbA to develop anti-bacterial agents to counteract virulence. DsbA is a structurally as well as functionally well characterized protein which has no direct homologue in eukaryotes. To date there are no known small molecule inhibitors of this protein. Hence DsbA represents an attractive antibacterial target and is the protein of interest for this project. The current thesis reports the identification of scaffolds that are suitable starting points for designing inhibitors of DsbA. These scaffolds were identified using a fragment-based drug design (FBDD) approach. This study describes a structure assisted-FBDD process performed to validate DsbA as an antibacterial drug target. A broad range of complementary techniques were employed to identify inhibitors of DsbA from two different Gram negative bacterial species; Escherichia coli and Vibrio cholerae. NMR based screening of a fragment library was carried out in two rounds. Theprimary screen employed STD (Saturation Transfer Difference) experiments to identify candidate hits and 1H-15N HSQC (Heteronuclear Single Quantum Coherence) experiments were used to confirm binding. A number of strong hits were identified and the binding locations were identified from the HSQC data. The binding efficiency of the fragments was determined by calculating NMR-based dissociation constants.Several of these initial binding fragments also show inhibitory activity in a phenotype assaysin E.coli. X-ray crystallographic studies revealed that the most potent fragment hits bind in the hydrophobic groove of EcDsbA in adjacent or overlapping positions. Charactisation of the structures of complexes of EcDsbA with theoriginal NMR hits was followed by fragment elaboration through structure-guided medicinal chemistry efforts. Preliminary structure-activity relationships(SAR) were identified for several series. This SAR will facilitate the development of novel DsbA inhibitors that specifically target functionally important protein surface sites. In conclusion, this study presentsthe discovery of novel small molecule inhibitors of DsbA and provides insights into the development of potential DsbA inhibitors as antibacterial drug candidates.
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  • Two-dimensional free-standing plasmonic superlattice (Plasmene) shows the superior ability to engineer properties by altering the particle size, shape, composition and interspacing. Despite the current success, there are still unsolved problems. This thesis aims to address issues from synthesis of plasmonic particles to fabrication of Plasmene using new building blocks and investigation on properties related applications. The successes in the synthesis of complex bimetallic nanoparticles and the fabrication of new plasmene nanosheets allow a better understanding of plasmonic coupling interactions of nanoparticles and self-assembly process. The demonstrated utilization of plasmene in immunoassay and optics further integrate the downstream real-world forensic applications.
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  • Online trolling has become omnipresent on the internet, in the media, and in popular culture. This thesis analyses the history of online trolling to reveal how this object of knowledge is structured through the dominant discourses of antagonism, deception and vigilantism, as well as any reconfiguration of these. In addition, given trolling is increasingly being directed at firms, brands and marketing communications online, this study explores how marketing industry professionals manage the threat of trolling. It reveals how consumers and marketers exercise specific strategies of control to instigate change through subtle, corrective, and pervasive means.
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  • The main subject of this thesis is the complete study of all the unpublished Ptah-Sokar-Osiris figures preserved in the Egyptian Museum of Florence. The research has taken into consideration typological, philological, and religious aspects of these particular funerary figures, also focusing on the reconstruction of their acquisition history using 19th Century manuscripts. The thesis includes a detailed catalogue of the artefacts. Department: Civiltà e Forme del Sapere University of Pisa - Italy Supervisor: Prof. Betrò, Marilina The thesis is under embargo until July 2052. https://etd.adm.unipi.it/theses/available/etd-06132012-235950/
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  • Laboratory experiments are an essential part of engineering education for students to gain practical skills and to prepare them for working in industries. However, due to the increasing number of undergraduate students and the rising cost of industry equipment, many universities are unable to provide industrial experience in the laboratories. To address these challenges, educators have proposed Virtual Laboratories (VLabs) that provide an alternative means of conducting experiments. This research proposes a specific type of VLab and investigates its significance in undergraduate engineering courses.
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  • This thesis tells the story of the experience of 18 teachers as they engaged in observing, deconstructing, and enacting teaching approaches associated with developing students’ reasoning and problem-solving. The findings confirm the potential and merit of modelling particular approaches to teaching to enhance mathematics teaching and learning by confronting teacher assumptions about teaching and learning and in doing so providing opportunities for them to develop mathematical knowledge for teaching. Two recommendations to improve pre-and post-modelled lesson briefings are presented.
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