Is it effective for patients taking dienogest to use progestin-primed ovarian stimulation (PPOS) protocol during controlled ovarian hyperstimulation (COH), compared to PPOS with dydrogesterone (DYG)?
Patients taking dienogest can continue the endometriosis treatment and get good quality embryo using PPOS during COH, despite they have severe ovarian endometriosis.
What is known already
Dienogest is an oral progestin effective for the treatment of symptomatic endometriosis, such as reduction of endometrial lesion and control of pain intensity with safe profile and good tolerability. Dienogest also provides complete ovulation inhibition at a daily dose of 2mg, and a rapid recovery of ovarian function after cessation of its administration. PPOS is a new COH regimen using a progestin as alternative to GnRH antagonist for blocking LH surge, and several reports have shown that DYG is an appropriate progestin for PPOS protocol. However, dienogest has not been used in PPOS protocol yet.
Study design, size, duration
This was a prospective controlled study of 145 women with endometriosis (aged <41) undergoing COH for IVF/ICSI and frozen embryo transfer (FET) at our infertility center from February in 2018 to November in 2019. The patients taking dienogest were allocated in Study group, and the other patients taking PPOS with DYG were allocated in Control group.
Participants/ Materials, setting, methods
A total of 145 patients were analyzed, PPOS with DNG: 71 patients, PPOS with DYG: 74 patients. Of the participants, 111 patients were histologically confirmed as endometriosis and 39 patients were diagnosed with published imaging criteria using transvaginal ultrasonography, respectively. Patients took DNG 2mg continuously in DNG group, and DYG were started day 3 of COH cycle. Patients were administrated with 150-225 IU of human menopausal gonadotropin (hMG) daily for COH. All viable embryos were cryopreserved for later transfer. The primary outcome measure was the clinical pregnancy rate.
The number of oocytes retrieved in DNG group was less than that of DYG group (6.18±3.60 vs. 9.85±5.77, P<0.001), however, the rate of mature oocytes in DNG group was significantly higher than in DYG group [89.1% (391/439) vs. 78.9% (575/729), P<0.001].The fertilization rate was comparable between the two groups (C-IVF; 69.0% for DNG group vs. 65.1% for DYG group, P=0.510, ICSI; 80.1% for DNG protocol vs. 78.2% for DYG group, P=0.558). The clinical pregnancy rate [Odds ratio (OR) 1.15, 95%CI: 0.69～1.94, P=0.579 ] :50.5% (54/107) for DNG group vs.46.8% (59/126) for DYG group. The ongoing pregnancy rate [OR 0.70, 95%CI: 0.45～1.61, P=0.323]:55.2% (37/67) for DNG group vs.63.6% (42/66) for DYG group did not differ between the two groups.
Contributors:mickish zhang, Dan-Ya Wu, Hui Zheng, Yao Wang, Qiao-Ran Sun, Xin Liu, Li-Yan Wang, Wen-Jing Xiong, Qiujun Wang, James D. P. Rhodes, Kai Xu, lijia li, zili LIN, Guang Yu, Weikun Xia, Bo Huang, zhenhai du, Yao Yao, Kim A. Nasmyth, Robert J. Klose, Yi-Liang Miao, Wei Xie
Somatic cell nuclear transfer (SCNT) can reprogram a somatic nucleus to a totipotent state. However, the re-organization of three-dimensional chromatin structure in this process remains poorly understood. Using low-input Hi-C, we revealed that during SCNT, the transferred nucleus first enters a mitotic-like state (premature chromatin condensation). Unlike fertilized embryos, SCNT embryos show stronger TADs at the 1-cell stage. TADs become weaker at the 2-cell stage, followed by gradual consolidation. Compartments A/B are markedly weak in 1-cell SCNT embryos and become increasingly strengthened afterward. By the 8-cell stage, somatic chromatin architecture is largely reset to embryonic patterns. Unexpectedly, we found cohesin represses minor zygotic genome activation (ZGA) genes (2-cell specific genes) in pluripotent and differentiated cells, and pre-depleting cohesin in donor cells facilitates minor ZGA and SCNT. These data reveal multi-step reprogramming of 3D chromatin architecture during SCNT and support dual roles of cohesin in TAD formation and minor ZGA repression.
Contributors:Joseph Kanja, Xiangwei Li, Andrew Hunter, Henry Brunskill, Rob Dwyer-Joyce
This data provides the reader with a sample of raw data and processed data which was used for the measurement of surface film thickness using a superimposed standing wave.
The reader is advised to refer to the research article "Non-contact measurement of the thickness of a surface film using a superimposed ultrasonic standing wave" for more information on this data and how to interpret it.
For the full database, please visit: www.projectipad.org
The 2011 accident at Japan’s Fukushima Daiichi Nuclear Power Plant released a considerable inventory of radioactive material into the local and global environments. While the vast majority of this contamination was in the form of gaseous and aerosol species, of which a large component was distributed out over the neighbouring Pacific Ocean (where is was subsequently deposited), a substantial portion of the radioactive release was in particulate form and was deposited across Fukushima Prefecture. To provide an underpinning understanding of the dynamics of this catastrophic accident, alongside assisting in the off-site remediation and eventual reactor decommissioning activities, the ‘International Particle Analysis Database’, or ‘IPAD’, was established to serve as an interactive repository for the continually expanding analysis dataset of the sub-mm ejecta particulate. In addition to a fully interrogatable database of analysis results for registered users (exploiting multiple search methods), the database also comprises an open-access front-end for members of the public to engage with the multi-national analysis activities by exploring a streamlined version of the data.
Do you lose control by investing in risky securities? Are low risky investments the best options for those who like to play it safe? Why do people speculate in risky assets? What is value investing and why do legends like Warren Buffet and Peter Lynch prefer value investing? This research will address these questions and will focus on busting the financial myth of “You need to speculate to accumulate”. It will analyze the returns generated by stocks having high risk and stocks having low risk linked to a real-time basis by taking the historical prices. The paper will conduct hypothesis testing regarding the statement proposed that whether risky investments generate higher returns or not. The research will give light on Low-volatility anomaly and will provide a solution in determining which kind of stock will be a safer bet to generate higher returns in a longer time frame. The research will consider the financials of the riskiest stocks in the past few years and will analyze their return accordingly.
This dataset consists of raw sequencing data (fasta) and analysed relative abundance data (histograms of the dominant 10 species in respective taxonomic ranks ). This project shows the first bacterial diversity profiling of high-microbial-abundance wild tropical marine sponges of southern South China Sea, which are Aaptos aaptos and Xestospongia muta from Bidong and Redang islands, Malaysia. Marine sponges are acknowledged as a bacterial hotspot and resource of novel natural products or genetic material. However, sponge-associated bacteria are difficult to be cultivated and the production of their desirable metabolites are inadequate in terms of rate and quantity, yet bioinformatics and metagenomics tools are progressing. Therefore, the diversity profiling of bacterial communities in marine sponges reveals the approximate gene pool for the gene mining or isolation of bacteria that are potentially and commercially beneficial in manufacturing industry, medicine, or agriculture. The bacterial community data exploited from this project is useful for critical comparison through additional or integrated bioinformatics processing with other marine sponge-associated bacterial community profile data. The community data of this project also unveils some general physiological function of the sponge-associated bacterial assemblage in its local environment. In the data provided, the sponge-associated bacterial communities in A. aaptos of Pulau Bidong, A. aaptos of Pulau Redang, and X. muta of Pulau Bidong have been denoted by A, B, and M, respectively.