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These datasets involve 1) ambient air quality testing, 2) spontaneous combustion fire frequency record, 3) temperature anomalies detected by Landsat, 4) photos of mine waste heap as well as affected environment, 5) estimation of remedial cost, 6) VDO of gas emission from a crack on top of the mine waste heap, and 7) XRD analysis of coal-mine waste
Data Types:
  • Other
  • Image
  • Video
  • Tabular Data
  • Dataset
  • Document
Smoke Test on 17Jul2019 natscilivecustomer (Dataset-1) Smoke Test on 17Jul2019 natscilivecustomer (Dataset-2)
Data Types:
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  • Software/Code
  • Image
  • Video
  • Tabular Data
  • Dataset
  • Document
  • Text
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Video S1 shows the process that the liquid level in the cylinder gradually decreased between 0 and 12 h, while the thickness of the bright thin layer at the bottom increased, which is the supplementary datum of Figure 1a. Video S2 presents that the gel, supported on the filter paper, can be obtained by decanting the upper suspension.
Data Types:
  • Video
  • Dataset
Supplemental material for publication titled - "Palm reading and water divining: A cross-sectional study of the accuracy of palmar hyper-linearity and trans-epidermal water loss to identify individuals with a filaggrin gene null mutation" to be published in Journal of the American Academy of Dermatology
Data Types:
  • Slides
  • Document
  • Dataset
Abolition of contractile activity in in vitro neuromuscular junctions within multi-nodal microfluidic chips using alpha-Bungarotoxin. Chips seeded as described above were allowed to mature for 21 days after seeding. Chips were selected for high contractile activity of myotubes that had visual contact with axons (more than 1 contraction per minute over 5 minutes with at least 1 contraction in each minute, the day prior to the experiment). Video of pre-experiment activity was recorded for 1 min followed by counting of the total number of contractions in 10 min. The cells were allowed to recover for 1 h at 37°C, 5% CO2 before addition of 1:100 of α-BTX at a final concentration of 1.25μM or of 0.75μl sterile PBS and incubation of 10 min at 37°C, 5% CO2. The α-BTX was only added to the central well containing the myotubes, which was fluidically isolated by hydrostatic pressure throughout the incubation. Another 1 min video of activity after intervention was recorded before counting the total number of contractions in 10 min. Cells were fixed immediately after the final count. For quantitative analysis the experiment was repeated with 10 min video being recorded as baseline followed by 1 h recovery at 37°C, 5% CO2 and treatment. Another 10 min video was recorded after treatment, and blinded quantification was carried out for both.
Data Types:
  • Video
  • Dataset
X chromosome inactivation in mammals is regulated by the non-coding (nc) RNA, Xist, which represses the chromosome from which it is transcribed. High levels of the N6-methyladenosine (m6A) RNA modification occur within Xist exon I, close to the 5’ end of the transcript, and also further 3’, in Xist exon VII. The m6A modification is catalysed by the METTL3/14 complex that is directed to specific targets, including Xist, by the RNA binding protein RBM15/15B. m6A modification of Xist RNA has been reported to be important for Xist–mediated gene silencing. We use CRISPR/Cas9 mediated mutagenesis to delete sequences around the 5’ m6A region in interspecific XX mouse embryonic stem cells (mESC). Following induction of Xist RNA expression we assay chromosome silencing using allelic RNA-seq and Xist m6A patterns using m6A-seq. Additionally we use Xist RNA FISH to analyse the effect of deleting the 5’ m6A region on the function of the endogenous Xist promoter. Purifying epitope tagged RBM15, we employ MS/MS analysis to define the RBM15 interactome in mESCs. We show that a deletion encompassing the entire Xist 5’ m6A region results in a modest reduction in Xist-mediated silencing, and that the 5’ m6A region overlaps essential DNA elements required for activation of the endogenous Xist promoter. Deletion of the Xist A-repeat, to which RBM15 binds, entirely abolishes deposition of m6A in the Xist 5’ m6A region without affecting the modification in exon VII. We show that in mESCs RBM15 interacts with the m6A complex, the SETD1B histone modifying complex, and several proteins linked to RNA metabolism
Data Types:
  • Other
  • Slides
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Appendix B. PET imaging 3D rendered images. 1. PET 3D of 4-89Zr 2. PET 3D of 5-89Zr
Data Types:
  • Video
  • Dataset
In 2017 and 2018, seventy-one individuals (33 females and 38 males) from 40 ranches across three Choyero communities in the Sierra de la Giganta of Baja California Sur, Mexico were presented an ethnobiological knowledge assessment task. As a first step of the task, individuals took a vision acuity test using “tumbling E” eye charts presented on a laptop computer (see TumblingE_EyeChart.pptx), a robust and easy to use diagnostic tool that is practical for populations with innumerate or analphabetic participants (Messina et al. 2006). From this test we derived a visual acuity score [1,…11]. A higher score indicates better visual acuity. The ethnobiological knowledge assessment task presented a sequence of 137 slides on a laptop computer featuring images of 87 plants and 50 animals (see EthnobiologicalKnowledgeAssessmentTool.pptx). The order of items presented was varied among participants. Assessments of ethnobiological knowledge were conducted in Spanish in the privacy of individual homes and occurred as part of a larger set of household interviews regarding ranching demography and lifestyles. These household interviews additionally informed us of individuals’ educational achievement, their ranch affiliation, and community membership. For each of 137 plant and animal images presented in sequence, the researcher (ES) asked the participant whether they recognized the item in the image. If the participant answered affirmatively, the researcher asked the follow-up question about what the item is called. Responses were recorded and coded “correctly named” if matching locally appropriate culturally correct names used for species identification (see Macfarlan et al., 2020 http://dx.doi.org/10.17632/kjds8jztzv.1). Here we provide raw data generated by seventy-one participants (33 females and 38 male). We also include a codebook that explains data types and meanings and we provide the .pptx files for tumbling E eye charts and the ethnobiological knowledge assessment task used to generate the data in this survey.
Data Types:
  • Slides
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(1) conti2d-win10.zip includes manual, source code and pre-compiled executable file with and without GUI for Win10 system. (2) conti2d-master.zip includes manual, source code for Linux and Mac OS. (3) Tutorial_conti2d.mp4 is a video tutorial for executable program of conti2d with GUI.
Data Types:
  • Video
  • Dataset
  • File Set
The concomitant change of the pH as acquired by the pH meter and the related color change of the sensor membrane
Data Types:
  • Video
  • Dataset