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  • We demonstrated that NK cell activity affects HSCs frequency in vitro as well as hematopoietic reconstitution in vivo. Gene expression and cytokine profile assays revealed the potential players of this HSC-NK cell regulation, such as IFNγ. Besides the known role of C/EBPg in NK cell differentiation, we demonstrated that it is also essential for NK-cytokine mediated HSC regulation. Finally, NK cell depletion from murine and human bone marrow favored transplant chimerism.
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  • The aim of this study is to identify sites occupied by Pdx1 throughout key stages in mouse and human pancreatic development as well as during in vitro differentiation of human ES cells.
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  • It is unclear why preterm birth increases risk of cardiovascular disease later in life. Studies in mice indicate excess oxygen used to treat preterm infants causes pulmonary hypertension, cardiac failure, and shortens lifespan. We previously reported neonatal hyperoxia causes pulmonary hypertension in aged mice as defined pathologically by pulmonary capillary rarefaction, dilation of pulmonary arterioles and veins, right ventricular hypertrophy, and reduced lifespan. Here, affymetrix gene arrays were used to identify early transcriptional changes in lungs of young adult mice exposed to room air or 100% oxygen between postnatal days 0-4.
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  • Elavl1/HuR is a ubiquitous and conserved RNA-binding protein that binds to a U-rich RNA motif that shuttles between nucleus and cytoplasm. In epithelia, the elevated expression of HuR assumingly promotes degeneration and cancer suggesting that its generic suppression may provide clinical benefits. In this study we focused on biological and clinical functions of HuR in intestinal epithelial cells and we presented evidence that changes in HuR levels induce polarized distortions in these cells to support different pathologic outcomes. For translation profiling cytoplasmic fractions of CMT93 sublines, containing monosomes and polysomes were separated as in (Katsanou et al., 2009). Fractions containing monosomes and polysomes were collected using a retriever 500 fraction collector (Teledyne Isco). Monosomal and polysomal fractions were pooled and total RNA was extracted from each fraction using Trizol reagent (Invitrogen). For micorarrays, RNAs were further purified via columns (Qiagen). Biotinylated complementary RNAs,(cRNAs) were hybridized onto Affymetrix Gene Mo-Gene-1.0 Chip in accordance to the protocols of the Genomics Unit of BSRC “Alexander Fleming”. (http://www.fleming.gr/facilities/genomics).
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  • This experiment aimed at characterising the signalling pathways downstream SUCNR1 activation in murine Neural Stem Cells (NSCs). To this end, we profiled by microarray the gene expression changes induced by succinate stimulation in both wild type NSCs and GPR91-deficient NSCs.
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  • Here we report the construction of a absA2 mutant strain which exhibited the classic precocious hyper-production of antibiotics and its complementation by an N-terminal triple-FLAG tagged version of AbsA2. The complemented and non-complemented absA2 mutant strains were used in large-scale time-course experiments to investigate the effect of deleting absA2 on gene expression at multiple time points and identify AbsA2 DNA binding sites in ChIP-on-chip studies using high-density microarrays.
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  • Here we report the construction of a absA2 mutant strain which exhibited the classic precocious hyper-production of antibiotics and its complementation by an N-terminal triple-FLAG tagged version of AbsA2. The complemented and non-complemented absA2 mutant strains were used in large-scale time-course experiments to investigate the effect of deleting absA2 on gene expression at multiple time points and identify AbsA2 DNA binding sites in ChIP-on-chip studies using high-density microarrays.
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  • This experiment aimed at characterising the modulatory role of murine induced Neural Stem Cells (iNSCs) and Neural Stem Cells (NSCs) on macrophages (MPs) exposed to LPS in vitro. Naïve MPs were polarized into an M1-like phenotype with LPS, and then co-cultured with 1:1 ratios of iNSCs in a trans-well system that avoids cell-to-cell contacts. Naïve MPs, LPS-stimulated MPs and LPS-stimulated MPs co-cultured with NSCs were used as controls.
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  • The experiment was designed to enable comparison between Arabidopsis thaliana columbia and DEWAX2 OX plants line.
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  • Desulfatibacillum alkenivorans AK-01 was grown on Hexadecane and Hexadecanoic acid. A microarray was run in triplicate for each growth condition and transcription was assessed for all predicted protein coding ORFs.
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