This research aims to provide an understanding of how absorptive capacity occurs in startups, which maintains interorganizational relationships with large companies. The research used a qualitative multiple case study design and the investigation of seven startups constituted the study corpus. The data were analyzed using statistical analysis, content analysis, and business process analysis. In the study, the software Iramuteq (Interface of R pour les Analyzes Multidimensionnelles de Textes et de Questionnaires) was used as a tool to aid the qualitative analysis of textual data. In a complementary way, the SPSS Statistics 17 software was used to treat the survey data.
It involves raw data and preprocessed files used for statistical analysis and the training of computational models. Please see the readme.txt files under each folder to get further information about the files inside that folder.
This repository includes a solidWorks (2019) CAD model of a research quality double pendulum. Additionally, we have provided video data with trackers recorded at 1000 FPS and Encoder data to validate the video data analysis.
Here we present the first demonstration of the use of eggshell membrane for research on endangered Maleo (Macrocephalon maleo).
We used 24 post-hatched eggshell membranes collected from two different sites, Tambun and Tanjung Binerean, in North Sulawesi, 12 samples in each. Two different DNA extraction methods: alkaline lysis method and gSYNCTM DNA Extraction Kit were applied. To determine the sex of Maleo, we utilized PCR-based DN A sexing using CHD genes, with the primer set 2550F/2718R.
We successfully extracted all samples; mean sample concentration was 267.5 ng/µl (range 47–510.5 ng/µl) and samples were of high purity (A 260/280 ratio 1.85±0.03). All samples were used to successfully identified sexes, 9 females and 15 males.
Our research clearly illustrates that eggshell membranes can be used for DNA sexing and open the possibility to build noninvasive DNA collections over large spatial scales for population study of endangered birds.
The data consist of
A. Electrophoresis photos of (1). DNA extraction of Maleo from eggshell membrane, collected from Tambun and Tanjung Binerean, North Sulawesi, from 4th April until 1st May 2018. We used gSYNCTM DNA Extraction Kit (Genaid) followed the provided user manual with little modifications to isolate the DNA frm the eggshell membrane.
The eluded DNA (1 µl) was quantified using NanoVue Plus™ (Biochrom, Harvard Bioscience, Inc), at A260 nm. The 260/280 nm absorbance ratio was measured to give an indication of purity of the DNA.
(2). PCR products of molecular sexing, using lysate and extracted DNA. We applied PCR based DNA sexing by using CHD genes, with the primer set 2550F/2718R (28). PCR used a 10 µl total volume containing template DNA (genomic DNA or lysate), 1.2 µl sterile dH2O, 5 µl 2x PCR buffer KOD FX Neo, 2 µl dNTPs (2 mM), (TOYOBO Co. Ltd.), 0.3 µl Primer 2550F (10 µM; 5'-GTT ACT GAT TCG TCT ACG AGA-3'), and 0.3 µl Primer 2718R (10 µM; 5'-ATT GAA ATG ATC CAG TGC TTG-3', (28)), and 0.2 U KOD polymerase enzyme. PCR was carried out in a Veriti™ 96-well thermal cycler (Applied Biosystems™). For genomic DNA templates, the following profile was used: 1 cycle at 94℃ for 2 min followed by 35 cycles of 98℃ for 10 sec, 53℃ for 30 sec and 68℃ for 45 sec;, and a final extension at 68℃ for 7 minutes. For lysate as DNA template, the PCR profiles was the same for DNA genome, except that it was run for more cycles (40x).
B. Sequences of CHD-W and CHD-Z genes of Maleo
The electrophoresis gels were cut on upper and lower bands for female sample and single band for male sample, then purified for sequencing. The sequence reactions were carried for both direction in sequencing services laboratory provided by 1st BASE Laboratories (Apical Scientific Sdn Bhd), Malaysia.
Repository of design files for the HardwareX article titled "A low-cost and customizable alternative for commercial implantable cannula for intracerebral administration in mice".
Contributing authors: Rogneda B Kazanskaya, Alexander V Lopachev, Tatiana N Fedorova, Raul R Gainetdinov, Anna B Volnova.
Abstract: Stereotaxic intracerebral cannula implantation for neuroactive agent administration is a wide-spread method for chronic experiments requiring bypassing the blood-brain barrier in rodents. However, commercially available cannula are bulky and may interfere with animal movement or lead to their dislodging during grooming. As the number of cannula needed in one experiment, and the accompanying costs can be high, it is in the interest of researchers to produce them on their own. Custom cannula manufacturing also offers the flexibility of different cannula lengths, which is required for agent delivery to various brain structures. In this article we present a protocol for making guide cannula along with the accompanying systems required for injection, which are small, cost-effective, light, easy to make, reusable, and can be made from easily procured materials.
Contributors:David Saceda-Corralo, Virginia Velasco Tamariz, Elena de las Heras, Sergio Vañó, Cristina Pindado
Supplemental figures of TRICHOSCOPIC FINDINGS OF DISCOID LUPUS ERYTHEMATOSUS ALOPECIA: A CROSS-SECTIONAL STUDY (an article in JAAD Journal). Brownish discolotation and follicular plugging in lupus alopecia.
Mass Spectrometry Imaging datasets used as validation of the functionality of rMSIcleanup (https://github.com/gbaquer/rMSIcleanup). Acquired with silver-assisted LDI using MALDI TOF/TOF ultrafleXtreme. Referred to as Dataset 1-10 in the accompanying publication (https://doi.org/10.1101/2019.12.20.884957). Datasets 1 and 2: Mouse Pancreatic Tissue. Dataset 3: Mouse Kidney Tissue. Datasets 4-10: Mouse Brain Tissue.
The data set contains Hadamard matrices of order 28 in machine-readable form, convenient for use in programs.
Data taken from the site
This data set complements following ones
Ukhalov, Alexey; Nevskii, Mikhail (2018), “Functions for checking necessary conditions for maximality of 0/1-determinant and example”, Mendeley Data, v1 http://dx.doi.org/10.17632/sm3x4xrb42.1
Ukhalov, Alexey (2019), “Matrices having the largest known determinant in machine-readable form”, Mendeley Data, v1 http://dx.doi.org/10.17632/hzf94h43c5.1
Data is presented in three formats: Wolfram Mathematica Notebook, PDF, and Plain Text.