Filter Results
10783 results
  • Dendritic Cell differentiation - CD molecule cluster follow up: The data files associated to this experiment show the gene expression levels for a subset of 152 transcripts (out of 12626 genes represented on Affymetrix Genechip HG_U95Av2) representing CD molecules specifically expressed in Dendritic Cells (DC) as assessed by the 9 conditions tested. Another subset of genes, corresponding to a cluster of Transcription regulators is available from E-MEXP-2 experiment.
    Data Types:
    • Software/Code
    • Tabular Data
    • Text
    • File Set
  • Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource for regenerative medicine. However, genetic manipulation and difficult-to-manufacture strategies used in reprogramming limit their clinical applications. Here, we show pluripotency can be induced from mouse somatic cells by specific small-molecule compounds. The completely chemically-induced pluripotent stem cells (CiPSCs) can be stably maintained in embryonic stem cell (ESC) culture medium and resemble ESCs in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. These findings suggest that exogenous master genes are dispensable for cell fate reprogramming and pave the way for the clinical application of somatic reprogramming techniques. Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource for regenerative medicine. However, genetic manipulation and difficult-to-manufacture strategies used in reprogramming limit their clinical applications. Here, we show pluripotency can be induced from mouse somatic cells by specific small-molecule compounds. The completely chemically-induced pluripotent stem cells (CiPSCs) can be stably maintained in embryonic stem cell (ESC) culture medium and resemble ESCs in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. These findings suggest that exogenous master genes are dispensable for cell fate reprogramming and pave the way for the clinical application of somatic reprogramming techniques. Chemicals' acronyms: V, VPA; C, CHIR; 6, 616452; T, tranylcypromine; F, FSK; Z, DZNep; P, PGE2; R, RG108; S, SRT1720; M, 2-Me-5HT; D, D4476; B, Sodium butyrate. To compare the global gene expression pofile of mouse embryonic fibroblasts (MEFs), mouse adult lung fibroblasts (MAFs), mouse neonatal fibroblasts (MNFs), adipose-derived stem cells (ADSCs), chemical induced pluripotent stem cells (CiPS), embryonic stem cells (ESCs) and OSKM-iPSCs. We profiled the mRNA expression of each sample by microarray (Mouse OneArray® v2, Phalanx). Different sample set was normalized for different purpose. Set1: MEFs1, CiPS34, MNF CIPS7, CiPS50 CiPS21, OSKM-iPS1 and ESCs1 were analyzed, each sample have three replicates; Set2: MNFs, MAFs, ADSCs, MNF CiPS1, MAF CiPS3, CiPS45, ADSC CiPS2, OSKM iPS2 and ESCs2 were analyzed, each sample have three replicates; Set3: WT MEFs, ESCs3, CiPS WT1 and CiPS WT2 were analyzed, each sample have two replicates; Set4: MEFs2, SKM-FSK1, SKM-FSK2 and ESCs4 were analyzed, each sample have three replicates; Set5: MEFs3, ESCs5, CiPSCs, D12, D20 and D32 were analyzed, each sample have two replicates.
    Data Types:
    • Document
    • Text
    • File Set
  • Gene transcription profiling in liver following high fat versus normal diet in C57/Bl6 mice. Characterisation of molecular mechanisms of in vivo insulin action in mouse models of experimentally induced Insulin Resistance. Switched the diet after mice are 5 weeks old, then sampled at 8 days, 3 weeks and 15 weeks.
    Data Types:
    • Software/Code
    • Geospatial Data
    • Image
    • Tabular Data
    • Text
    • File Set
  • PAO1 was grown in sub-inhibitory ciprofloxacin (0.1x-, 0.3x-, 1x-MIC) until log phase. Microarrays were done on total RNA isolated from these cultures. Loop design and dye swapping were used.
    Data Types:
    • Software/Code
    • Geospatial Data
    • Image
    • Tabular Data
    • Text
  • Mice which lack IRS-2, develop insulin resistance. Comparison between white adipose tissue from 5 week old IRS-2 KO and wild type mice. Processed data is not included for this experiment and therefore these data are not MIAME compliant
    Data Types:
    • Software/Code
    • Geospatial Data
    • Image
    • Tabular Data
    • Text
  • Study comprising 74 kidney tumor samples of different histological type, differentiation grade, stage and with data on chromosomal aberrations and follow-up. Samples were hybridised against a common reference obtained from pooling different kidney tumor samples.
    Data Types:
    • Software/Code
    • Geospatial Data
    • Image
    • Tabular Data
    • Text
    • File Set
  • Gene transcription profiling in muscle following muscle versus normal diet in C57/Bl6 mice. Switched the diet when mice were 5 weeks old, then sampled at 8 days, 3 weeks and 15 weeks.
    Data Types:
    • Software/Code
    • Geospatial Data
    • Image
    • Tabular Data
    • Text
    • File Set
  • Gene transcription profiling in Epidydimal fat pad (EPD) following High fat versus normal diet in C57/Bl6 mice. Characterisation of molecular mechanisms of in vivo insulin action in mouse models of experimentally induced Insulin resistance. Switched the diet when mice were 5 weeks old, then sampled at 8 days.
    Data Types:
    • Software/Code
    • Geospatial Data
    • Image
    • Tabular Data
    • Text
    • File Set
  • Finally differentiated 3T3-L1 adipocytes are treated with insulin (0 or 100nM)or metformin (0 or 2mM)for 2 and 12 hours to understand insulin and metformin(an anti-diabetic drug commonly applied for Non-Insulin Dependent Diabetes Mellitus)action in adipose tissues.
    Data Types:
    • Software/Code
    • Geospatial Data
    • Image
    • Tabular Data
    • Text
  • Gene transcription profiling in Epidydimal fat pad (EPD) following High fat versus normal diet in C57/Bl6 mice. The diet was switched when mice were 5 weeks old, then samples taken at 2 days, 8 days, 3 weeks and 15 weeks.
    Data Types:
    • Software/Code
    • Geospatial Data
    • Image
    • Tabular Data
    • Text
    • File Set