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Pacemaker neurons exert control over neuronal circuit function by their intrinsic ability to generate rhythmic bursts of action potential. Recent work has identified rhythmic gut contractions in human, mice and hydra to be dependent on both neurons and the resident microbiota. However, little is known about the evolutionary origin of these neurons and their interaction with microbes. In this study, we identified and functionally characterized prototypical ANO/SCN/TRPM ion channel expressing pacemaker cells in the basal metazoan Hydra by using a combination of single-cell transcriptomics, immunochemistry, and functional experiments. Unexpectedly, these prototypical pacemaker neurons express a rich set of immune-related genes mediating their interaction with the microbial environment. Functional experiments validated a model of the evolutionary emergence of pacemaker cells as neurons using components of innate immunity to interact with the microbial environment and ion channels to generate rhythmic contractions. Data includes: Full count matrices for all the plates: - SS_038.rsem_counts.txt.gz - SS_039.rsem_counts.txt.gz - SS_040.rsem_counts.txt.gz Full expression matrix after cell filtering: raw_count_table_seurat_filter_cluster_ID_191009_SG.txt.gz Metadata with cluster assignment: cell_seurat_clusters_identity.txt.gz
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Time series single cell RNA sequencing analysis of smooth muscle cell (SMC) to mesenchymal cell transition process in TGFβR2iSMC-Apoe-/- and Apoe-/- mice. Chromatin immunoprecipitation DNA sequencing was done to identify the potential binding sites of DNA-associated proteins in human aortic smooth muscle cells treated with TGFb or PBS.
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Excel and other file sources for the Golden Droplets series
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Characterization of TGFβR2iSMC-Apoe-/- mice after 1 month of high cholesterol high fat by histology, imaging mass cytometry (IMC), histoCAT, CyTOF, and immunocytochemistry. Aortic smooth muscle cells from TGFβR2iSMC-Apoe-/- express stem cell markers (CD105, CD73, CD90, Sca-1, CD44), mesenchymal cell markers (Osteopontin, Aggrecan, Adiponectin). To show clonal origin of smooth muscle cell-derived mesenchymal cells in TGFβR2iSMC-Apoe mice-/-, we replaced mTmG reporter strain TGFβR2iSMC-Apoe mice generating Myh11CreERT2;Tgfbr2f/f;Apoe-/-;Confettif/f and Myh11CreERT2;Apoe-/-:Confettif/f (control) lines.
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Data Related to Fig 2. A mouse line carrying floxed Tgfbr2 and SMC lineage-tracing mT/mG alleles under control of a myosin heavy chain (Myh11) promoter on an Ldlr null background (Ldlr-/-;Myh11CreERT2;mT/mGf/f;Tgfbr2f/f) hereafter called TGFβR2iSMC-Ldlr. These mice were treated with Tamoxifen at 6 week of age and fed high cholesterol high fat diet for 4 months. Ascending aorta smooth muscle cells from these mice express macrophage, chondrocyte, adipocyte, and osteoblast lineage markers as shown by immunocytochemistry and qRT-PCT from laser micro dissection tissues compared to controls.
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In the current data article, we present detailed characteristics of voids in carbon/epoxy composite laminates as well as the original image stacks, obtained via X-ray micro-Computed Tomography (micro-CT) . Five different lay-ups are produced with altering the recommended cure cycle in order to intentionally induce voids in the material. For each lay-up, an image stack (consisting of tomographic slices) and a dataset are provided. The image slices are in 8-bit TIF format. The datasets (spreadsheets) include the volume, size parameters, shape parameters, orientation, and location of all the detected voids in the specimen. The segmentation of the images and quantification of voids are performed in VoxTex, an in-house software for processing of micro-CT results. The data is linked to a Data in Brief article "A dataset of voids’ characteristics in multidirectional carbon fiber/epoxy composite laminates, obtained using X-ray micro-computed tomography" and linked to the article "Mehdikhani et al. Detailed characterization of voids in multidirectional carbon fiber/epoxy composite laminates using X-ray micro-computed tomography. Comp Part A. in press.".
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In the current data article, we present detailed characteristics of voids in carbon/epoxy composite laminates as well as the original image stacks, obtained via X-ray micro-Computed Tomography (micro-CT) . Five different lay-ups are produced with altering the recommended cure cycle in order to intentionally induce voids in the material. For each lay-up, an image stack (consisting of tomographic slices) and a dataset are provided. The image slices are in 8-bit TIF format. The datasets (spreadsheets) include the volume, size parameters, shape parameters, orientation, and location of all the detected voids in the specimen. The segmentation of the images and quantification of voids are performed in VoxTex, an in-house software for processing of micro-CT results. The data is linked to a Data in Brief article "Mehdikhani et al., A dataset of voids’ characteristics in multidirectional carbon fiber/epoxy composite laminates, obtained using X-ray micro-computed tomography, DIB 27 (2019) 104686" and linked to the article "Mehdikhani et al. Detailed characterization of voids in multidirectional carbon fiber/epoxy composite laminates using X-ray micro-computed tomography. Comp Part A 125 (2019) 105532".
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Raw data for experimentation of photosensitivity of fungal substrates
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Smooth muscle cell (SMC) TGFb signaling and expression of relevant genes in the media of normal human aortas and non-Marfan atherosclerotic ascending aorta aneurysms by bulk RNAseq and immunocytochemistry. Related To Fig 1. SMC aneurysms samples show a reduction in TGFb signaling and increased expression of inflammation-related genes compared to normal aortas SMCs.
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