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Pacemaker neurons exert control over neuronal circuit function by their intrinsic ability to generate rhythmic bursts of action potential. Recent work has identified rhythmic gut contractions in human, mice and hydra to be dependent on both neurons and the resident microbiota. However, little is known about the evolutionary origin of these neurons and their interaction with microbes. In this study, we identified and functionally characterized prototypical ANO/SCN/TRPM ion channel expressing pacemaker cells in the basal metazoan Hydra by using a combination of single-cell transcriptomics, immunochemistry, and functional experiments. Unexpectedly, these prototypical pacemaker neurons express a rich set of immune-related genes mediating their interaction with the microbial environment. Functional experiments validated a model of the evolutionary emergence of pacemaker cells as neurons using components of innate immunity to interact with the microbial environment and ion channels to generate rhythmic contractions. Data includes: Full count matrices for all the plates: - SS_038.rsem_counts.txt.gz - SS_039.rsem_counts.txt.gz - SS_040.rsem_counts.txt.gz Full expression matrix after cell filtering: raw_count_table_seurat_filter_cluster_ID_191009_SG.txt.gz Metadata with cluster assignment: cell_seurat_clusters_identity.txt.gz
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this Data reveals all results founded in this research paper
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Palynomorph assemblages and palynofacies analysis have been performed on several core samples from the Devonian–Carboniferous deposits identified in five wells located in the eastern part of the Moesian Platform. The investigated sections include, in ascending stratigraphic order, the Ţăndărei, Smirna, Călăraşi and Vlaşin formations. Based on stratigraphic distribution of key taxa identified (miospores, chitinozoans, acritarchs), seven biozone intervals (four for Devonian and three for Carboniferous) have been recognized. The oldest samples were dated as being part of the micrornatus-newportense (MN) – lower part of breconensis-zavallatus (BZ) interval zones (early Devonian), while the younger ones are assigned to the kosankei-varioreticulatus (KV) – nobilis-junior (NJ) interval zones (late Carboniferous). Palynofacies observations suggest a more distal depositional environment during the period between Lochkovian and Pragian times, followed by some proximal/fluvio-deltaic conditions in Emsian–early Eifelian (the top of Ţăndărei Formation). The upper Tournaisian to Serpukhovian sedimentary rocks of the Călăraşi and lower part of Vlaşin formations were deposited in inner neritic environments. Mud-dominated dysoxic/anoxic conditions prevailed in the Bashkirian, which were quickly succeeded by a deltaic deposition and oxidizing environments which persisted up to Moscovian. The lower Devonian terrestrial palynoflora is dominated by trilete spores which belong to the lowland vegetation of a non-forest mire palaeoecological group. The Carboniferous deposits yielded only terrestrial palynomorphs of various types of arborescent and herbaceous lycopsids and ferns, suggesting different habitats such as non-flooded wetlands or swamps within coastal plain and continental interiors. These assemblages of Carboniferous miospores are an indication of the neutral-humid climatic conditions which existed at the time of deposition.
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In the current data article, we present detailed characteristics of voids in carbon/epoxy composite laminates as well as the original image stacks, obtained via X-ray micro-Computed Tomography (micro-CT) . Five different lay-ups are produced with altering the recommended cure cycle in order to intentionally induce voids in the material. For each lay-up, an image stack (consisting of tomographic slices) and a dataset are provided. The image slices are in 8-bit TIF format. The datasets (spreadsheets) include the volume, size parameters, shape parameters, orientation, and location of all the detected voids in the specimen. The segmentation of the images and quantification of voids are performed in VoxTex, an in-house software for processing of micro-CT results. The data is linked to a Data in Brief article "Mehdikhani et al., A dataset of voids’ characteristics in multidirectional carbon fiber/epoxy composite laminates, obtained using X-ray micro-computed tomography, DIB 27 (2019) 104686" and linked to the article "Mehdikhani et al. Detailed characterization of voids in multidirectional carbon fiber/epoxy composite laminates using X-ray micro-computed tomography. Comp Part A 125 (2019) 105532".
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The dataset provides a chaos game representation (CGR) of SARS-CoV-2 virus nucleotide sequences. The dataset is composed of 100 virus instances of SARS-CoV-2. In addition, the dataset also provides a CGR representation of 11540 viruses from the Virus-Host DB dataset and the other three Riboviria viruses from NCBI.
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There is a detailed Readme.pdf in the files for the informations about the dataset. The main purpose is providing a dataset for the vibration behavior of a robot manipulator system under the control input of model-associative vibration control (MAVC) prodecure. Velocity profile is shown as [∗,𝑡𝑐𝑜𝑛,𝑡𝑑𝑒𝑐,𝑡𝑚] in study. In the case studies for both simulations and experiments, the parameters are varied as follows; 𝑡𝑐𝑜𝑛 can be valued as 0, 𝑡1ℎ or 2𝑡1ℎ, 𝑡𝑑𝑒𝑐 can be valued as 𝑡1ℎ,2𝑡1ℎ,3𝑡1ℎ,4𝑡1ℎ or 5𝑡1ℎ and 𝑡𝑚 can be valued as 1 or 1.5 seconds for corresponded 90 or 135 angular displacements. Thus thirty different velocity profiles are produced with aim to performed on system. Cases are invastigated with and without performing the MAVC procedure. Than the robot manipulator is examined for both unloaded and loaded cases, therefore total one hundred twenty cases are occured. More details can be found in related study.
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Western blots and soft agar images
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This data set contains the derived data products for the published manuscript Orgel et al. (2020) "Re-examination of the population, stratigraphy, and sequence of mercurian basins: Implications for Mercury´s early impact history and comparison with the Moon" at Journal of Geophysical Research Planets. This data package includes GIS-ready shapefiles of Mercurian basins, crater counting measurements, and Craterstats files.
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Identifying Factors Affecting E-customer Loyalty in Gamified Trusted Store Platforms: A Case Study Analysis in Iran
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The micro x-ray CT images of two rock samples have been reconstructed. The data type of images: uint8 Spatial Resolution: 1.2 micrometer/voxel Size: 400X400X400 The reconstructed or "Original" images are suffering from existence of artifacts, roundoff errors, and different types of visual or mathematical noises in the reconstructed CT images. Each image has been applied with a series of bandpass and bilateral filters.
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