Filter Results
102538 results
Test
Data Types:
  • Video
  • Dataset
Hardware design for build a Step Width System Capture
Data Types:
  • Other
  • Software/Code
  • Geospatial Data
  • Image
  • Sequencing Data
  • Tabular Data
  • Dataset
  • Document
  • Text
  • File Set
  • Audio
Different human linker histone (H1) variants are expected to have distinct binding modes to the nucleosome. The position and orientation of a number of different H1 globular domains on the nucleosome were investigated through molecular docking using MGLTools and HADDOCK. The nucleosome core and linker DNA in the GH5-chromatosome structure (PDB: 4QLC) were used as a docking template. GH5 (in PDB: 4QLC) was re-docked to this template to test the docking algorithm. Docked and re-docked GH5 compared well. The docking algorithm was further tested by docking the NMR solution structure of the globular domain of chicken H1 (GH1, PDB: 1GHC) to the nucleosome template. The position of docked GH1 on the nucleosome agreed with literature. 
The N-terminal - and globular domain H1x hybrid (NGH1x) was studied using solution NMR in both low (20 mM sodium phosphate, pH 7.0) and high (20 mM sodium phosphate, 1 M sodium perchlorate, pH 7.0) ionic strength conditions (de Wit, H., Vallet, A., Brutscher, B. et al. Biomol NMR Assign (2019) 13: 249. https://doi.org/10.1007/s12104-019-09886-x). These low and high ionic strength structures were docked to the nucleosome template. 
Homology (MODELLER) and ab initio modeling (CS-ROSETTA) were employed to model structures for other human H1 globular domains: GH1.0, GH1.4, GH1oo, and GH1t. The modeled structures were also docked to the nucleosome template.
 All the docking procedures listed above produced 100 models of different energies. In each case, the lowest energy docked model was chosen. The structures of all the H1 globular domains that were docked to the template are given as PDB files (1GHC_lowest_energy.pdb; 2LSO_lowest_energy.pdb; GH5_re-docked_position.pdb; NGH1x_high_salt_NTD.pdb; NGH1x_low_salt_NTD.pdb; modeled_GH1_0_lowest_energy.pdb; modeled_GH1_4_lowest_energy.pdb; modeled_GH1oo_lowest_energy.pdb; modelled_GH1t_lowest_energy.pdb) in the data file. The nucleosome template structure is also given in PDB file format (4QLC_nucleosome_without_GH5.pdb). Finally, the docked models are also given (GH5-chromatosome.pdb; 1GHC-chromatosome.pdb; 2LSO-chromatosome.pdb; GH1_0-chromatosome.pdb; GH1_4-chromatosome.pdb; GH1oo-chromatosome.pdb; GH1t-chromatosome.pdb; NGH1x_no_salt-chromatosome.pdb; NGH1x_salt-chromatosome.pdb). The files are compatible with most molecular graphics software. The file Dockings_modelling_test_and_results.pdf provides the modeling and docking results in figures and tables. A short description of each figure and table is given within the PDF file.
Data Types:
  • Sequencing Data
  • Dataset
  • Document
Variant call file of DENV2 16681 passage 1 in M3 iPSC cells (open with Microsoft Excel)
Data Types:
  • Sequencing Data
  • Dataset
The Indeterminate Domain (IDD) proteins are a plant specific subclass of C2H2 Zinc Finger transcription factors. Some of these transcription factors play roles in diverse aspects of plant metabolism and development; however the function of most of IDDs is unknown and its molecular evolution has not been explored. Here, Prochetto and Reinheimer reconstructed the evolution of IDDs during plant land conquest. They found that IDDs arose from the common ancestor of Streptophyta. Once in land, IDDs experienced a rapid radiation that accompanied key morphological, physiological and biochemical transitions required in plant terrestrialization. The authors present a solid phylogenetic framework of annotated IDD genes which links genetic and functional knowledge from model to non-model species.
Data Types:
  • Other
  • Sequencing Data
  • Tabular Data
  • Dataset
  • Text
Data from: Bitomský M., Mládková P., Pakeman RJ, & Duchoslav M. (2020). Clade composition of a plant community indicates its phylogenetic diversity. Ecology and Evolution. doi: 10.1002/ece3.6170 Data summarises results from the case studies and simulations presented in our paper. In addition, we provide an R script for calculation of proposed phylogenetic diversity metrics (the clade indices). Brief description of each file: 1. Grasslands_DNA_markers_info.xls - Accession numbers of all DNA markers used for phylogeny inference in grasslands 2. Grasslands_DNA_alignment_BEFORE_GBlocks.fasta - DNA alignment matrix before utilisation of the GBlocks tool 3. Grasslands_DNA_alignment_AFTER_GBlocks.fasta - DNA alignment matrix after utilisation of the GBlocks tool 4. Grasslands_BEAST_file.xml - BEAST .xml file submitted to the CIPRES portal (www.phylo.org) 5. Grasslands_tree.txt - Dated MCC tree, grasslands (newick format) 6. Grasslands_tree.nex - Dated MCC tree, grasslands (nexus format) 7. Phyto-database_pruned_tree.txt - Pruned dated tree from the super tree of European flora (Durka & Michalski 2012, Ecology), phytosociological database (newick format) 8. Plot_data.slx - plot data of all case studies + species lists 9. Simulation_results.txt - Summary of R2 values (phylogeny-based metric ~ the clade index) for simulated phylogenies and community matrices (manipulated: phylogenetic scale, species pool size and species richness range) 10. Bitomsky2020EE_R_script_indices.R - An R script for computation of the clade indices (with notes and examples)
Data Types:
  • Software/Code
  • Sequencing Data
  • Tabular Data
  • Dataset
  • Document
  • Text
Alignments for phylogenetic analyses
Data Types:
  • Sequencing Data
  • Dataset
Time evolution of melt pool / plasma region shown together with time-dependent phase changes determined from interference fringence: "diff phase overlay.mp4" The color map goes from -2Pi (blue) to +14Pi (red), see "image sequence with legends.jpg"
Data Types:
  • Image
  • Video
  • Dataset
This is a video clip of the microdischarges status in the entire plasma electrolytic oxidation process
Data Types:
  • Video
  • Dataset
The dataset includes ray characteristics obtained from conventional shooting and bouncing simulations. The nominal and numeric versions are available.
Data Types:
  • Video
  • Tabular Data
  • Dataset
  • File Set