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l-lactate plays an important role as metabolite and signaling molecule in eukaryotes and bacteria. Monocarboxylate transporters (MCTs) of the SLC16 solute carrier family are responsible for the transport of l-lactate across eukaryotic and bacterial cell membranes. Here we report an efficient protocol for the expression and purification of an SLC16 family homologue in milligram amounts. The purified protein is stable and can thus be used for biochemical and structural studies as shown by successful crystallization.
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Kaposiform hemangioendothelioma (KHE) is a rare infiltrative vascular tumor that may be associated with Kasabach-Merritt Phenomenon (KMP), which is a consumptive coagulopathy with potentially life-threatening thrombocytopenia. Management of KHE and KMP is challenging, and currently, there are no standardized validated treatment protocols. Mammalian target of rapamycin inhibitors have been shown to be effective in the treatment of KHE. We describe a term male who presented as a diagnostic dilemma with life-threatening pleural and pericardial effusions and severe thrombocytopenia. After extensive work-up the etiology for his condition was determined to be KHE with KMP. The patient was commenced on sirolimus and responded well to therapy with resolution of KMP.
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Digital value-added services (DVAS) represent a major opportunity for firms to create additional value for customers and differentiate themselves from competitors. However, many firms are struggling with how to develop DVAS. They often hire specialized innovation consulting firms to do this job but we know little about their secrets of success. To shed more light on the topic, we collected best practices along the critical first steps of DVAS development and derived six recommendations that can help firms increase their success rates. We provide insights, particularly concerning underlying organizational processes, the conception of ideas and the integration of customers.
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The Novgorod birchbark letters occupy an intermediate position on a continuum between orality and literacy. Thanks to this corpus, we can trace the development of a formal written language over a period of more than four centuries. Its emergence can partly be traced to (1) the adaptation of Church Slavonic norms to secular text types, and partly be attributed to (2) an adaptation of vernacular oral habits to the written medium. The twofold origin of this development is presented by means of a case study, viz. the use of verbal tenses, especially the perfect and aorist, in performative formulae. In early texts, the use of the aorist is due to Church Slavonic influence; in later texts, on the contrary, it is due to the development of a “language of distance” in interaction with patterns of oral influence. A comparison is made between the birchbark letters and a representative sample of parchment letters from Novgorod and Pskov. The use of the aorist in performative formulae is also attested in Ancient Greek and in Old Church Slavonic translations from Greek. Thus, a comparison can be made both within and beyond the Slavic realm. It is argued that a passive command of Church Slavonic led to a reanalysis of the aorist as a stylistically higher variant of the Old Russian perfect tense. This perception gained momentum in concordance with the growing status of the written word and the increasing “performative” role of written documents.
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OBJECTIVE To evaluate the effect of the timing of secondary alveolar bone graft (SABG) on craniofacial morphology in patients with complete unilateral cleft lip and palate (UCLP). DESIGN Single-center retrospective assessment of consecutively treated nonsyndromic patients with complete UCLP. PARTICIPANTS One hundred sixty-seven patients (108 males, 59 females) with complete UCLP in whom the cleft was repaired with 1-stage method at approximately 8 months of age. The age of 128 patients at SABG varied from 1.4 to 11.5 years (SABG group), while 39 patients still awaited SABG at the moment of cephalometric evaluation (no-SABG group). METHODS Craniofacial morphology was assessed on lateral cephalograms taken at 10 years of age (standard deviation = 0.8; range: 7.5-12.3) using linear and angular measurements. tests and regression models were made to analyze data. RESULTS Regression models demonstrated that the effect of SABG on the craniofacial morphology was limited-cephalometric variables which were statistically significantly different between SABG and no-SABG groups showed no association with the timing of SABG when (1) age of primary repair of the cleft, (2) age of cephalometric evaluation, (3) cleft side, (4) gender, and (5) operator were controlled for. Only the length of the maxilla (Condylion-point A) was affected-1-year delay of SABG corresponded with an increase in Co-point A distance by 0.52 mm. However, adjusted of the model was 0.11. CONCLUSIONS Our findings cautiously indicate that SABG performed before 8 years of age can have limited negative effect on craniofacial morphology. Nevertheless, our results should be confirmed by cleft centers practicing alternative surgical repairs of the cleft.
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Individuals’ disclosure of personal health information (PHI) can hold substantial benefits for both users and providers, but users are often reluctant to disclose, even if they gain benefits such as better personalization. While previous research has dealt with message framing and information quality in a health related context, factors have been observed separately. To our best knowledge, we are among the first to have examined both factors (attribute framing and argument strength) and their interactions concerning PHI disclosure. Thus, we conducted a web based experiment with 529 participants to examine the impacts of two persuasive message techniques (attribute framing and argument strength) on individuals’ PHI disclosure. We reveal that individuals tend to disclose more PHI when they experience persuasive messages with more positively framed health wearable (HW) attributes or messages with higher argument strength concerning data collection. We enable researchers to uncover the impacts of persuasive messages in highly sensitive data environments and provide practitioners with workable suggestions on how to affect individuals’ PHI disclosure behaviors.
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