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Pacemaker neurons exert control over neuronal circuit function by their intrinsic ability to generate rhythmic bursts of action potential. Recent work has identified rhythmic gut contractions in human, mice and hydra to be dependent on both neurons and the resident microbiota. However, little is known about the evolutionary origin of these neurons and their interaction with microbes. In this study, we identified and functionally characterized prototypical ANO/SCN/TRPM ion channel expressing pacemaker cells in the basal metazoan Hydra by using a combination of single-cell transcriptomics, immunochemistry, and functional experiments. Unexpectedly, these prototypical pacemaker neurons express a rich set of immune-related genes mediating their interaction with the microbial environment. Functional experiments validated a model of the evolutionary emergence of pacemaker cells as neurons using components of innate immunity to interact with the microbial environment and ion channels to generate rhythmic contractions. Data includes: Full count matrices for all the plates: - SS_038.rsem_counts.txt.gz - SS_039.rsem_counts.txt.gz - SS_040.rsem_counts.txt.gz Full expression matrix after cell filtering: raw_count_table_seurat_filter_cluster_ID_191009_SG.txt.gz Metadata with cluster assignment: cell_seurat_clusters_identity.txt.gz
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We describe data that has been collected and analysed from the nutritional status of children (2-5 years) in the Tamale Metropolis, Tamale – Ghana.
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Background: School-aged children are increasingly engaging with multiple conflicting texts to understand complex societal issues, however empirical research has not yet examined in what ways contextual factors affect detection of and memory for conflicts. Methods: The current experiment manipulated contextual factors that included the vocabulary terms that authors of different texts used when describing the same concepts, and the order with which students accessed contradictory information. Results: After controlling for general science knowledge, adolescent students displayed longer reading times when contradictory stances were presented in an alternating fashion than they did when texts were blocked by stance. When text presentation was alternating, students also remembered more conflicts when the texts used the same vocabulary terms than they did when the texts used different vocabulary terms (non-obvious synonyms). However, when adolescents read texts blocked by stance, they remembered a similar number of intertextual conflicts regardless of whether texts used the same or different vocabulary. Conclusions: The findings suggest that different contextual factors can facilitate (but also undermine) propensities to notice and remember conflicts across texts. As such, the findings have important implications for theories of text comprehension and applications for adolescents’ everyday reading experiences. Disclaimer: These materials are not to be redistributed or repurposed without permission from the corresponding author.
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Time series single cell RNA sequencing analysis of smooth muscle cell (SMC) to mesenchymal cell transition process in TGFβR2iSMC-Apoe-/- and Apoe-/- mice. Chromatin immunoprecipitation DNA sequencing was done to identify the potential binding sites of DNA-associated proteins in human aortic smooth muscle cells treated with TGFb or PBS.
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The files contain the replication data and replication instructions (as STATA do-file) that replicate the results presented in the article: Lujala, Päivi, and Christa Brunnschweiler and Ishmael Edjekumhene 2020. Transparent for whom? Dissemination of information on Ghana’s petroleum and mining revenue management. Journal of Development Studies. The article is an open access article and you can access it through this link: https://doi.org/10.1080/00220388.2020.1746276 The dataset comes from a survey conducted in Ghana between June-August 2016. The purpose of the survey was to study people’s level of knowledge of and perceptions and attitudes towards a number of issues related to petroleum and mining revenue management and to understand how people inform themselves about such matters. The survey sample consists of 3526 adult (18 years and over) respondents, who were interviewed face-to-face. The survey was conducted in 120 of the 216 districts in Ghana at the time (currently Ghana has 260 districts).
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Characterization of TGFβR2iSMC-Apoe-/- mice after 1 month of high cholesterol high fat by histology, imaging mass cytometry (IMC), histoCAT, CyTOF, and immunocytochemistry. Aortic smooth muscle cells from TGFβR2iSMC-Apoe-/- express stem cell markers (CD105, CD73, CD90, Sca-1, CD44), mesenchymal cell markers (Osteopontin, Aggrecan, Adiponectin). To show clonal origin of smooth muscle cell-derived mesenchymal cells in TGFβR2iSMC-Apoe mice-/-, we replaced mTmG reporter strain TGFβR2iSMC-Apoe mice generating Myh11CreERT2;Tgfbr2f/f;Apoe-/-;Confettif/f and Myh11CreERT2;Apoe-/-:Confettif/f (control) lines.
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Test
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Data Related to Fig 2. A mouse line carrying floxed Tgfbr2 and SMC lineage-tracing mT/mG alleles under control of a myosin heavy chain (Myh11) promoter on an Ldlr null background (Ldlr-/-;Myh11CreERT2;mT/mGf/f;Tgfbr2f/f) hereafter called TGFβR2iSMC-Ldlr. These mice were treated with Tamoxifen at 6 week of age and fed high cholesterol high fat diet for 4 months. Ascending aorta smooth muscle cells from these mice express macrophage, chondrocyte, adipocyte, and osteoblast lineage markers as shown by immunocytochemistry and qRT-PCT from laser micro dissection tissues compared to controls.
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dataset for helping and job performance dilemma study
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