Effective spatio-temporal control of transcription and replication during S-phase is paramount to maintaining genomic integrity and cell survival. Dysregulation of these systems can lead to conflicts between the transcription and replication machinery causing DNA damage and cell death. BRD4, a BET bromodomain protein and known transcriptional regulator, interacts with P-TEFb to ensure efficient transcriptional elongation by stimulating phosphorylation of RNA Polymerase II (RNAPII). Here we report that disruption of BET bromodomain protein function causes RNAPII pausing on the chromatin and DNA damage affecting cells in S-phase. We find that this persistent, RNAPII-dependent pausing leads to accumulation of RNA:DNA hybrids (R-loops), which are known to lead to transcription-replication conflicts (TRCs), DNA damage, and cell death. Furthermore, we show that resolution of R-loops abrogates BET bromodomain inhibitor-induced DNA damage, and that BET bromodomain inhibition induces both R-loops and DNA damage at sites of BRD4 occupancy. Finally, we see that the BRD4 C-terminal domain, which interacts with P-TEFb, is required to prevent R-loop formation and DNA damage caused by BET bromodomain inhibition and that oncogenes which promote transcription and replication exacerbate BET bromodomain inhibitor-induced DNA damage. Together, these findings demonstrate that BET bromodomain inhibitors can damage DNA via induction of R-loops and TRCs in highly replicative cells.
CT evaluation of TKA in 02 different implant designs , PFC sigma and Genesis II, after implantation, to verify the external rotation of the femoral component achieved with reference to the TEA.Genesis II has a Fixed inbuilt external rotation of 3 degrees and the PFC sigma can be modified. The data highlights the effective femoral external rotation achieved and its possible impact on patellofemoral kinematics
By optimizing for a reduction in peak line loading in a typical benchmark 0.4 kV network, additional line loading percentages caused by the introduction of incremental increases in the number of EVs charging in an agent based Monte Carlo simulation can be reduced by over 10% in the worst case and about 5% on average across several cable types. Optimization for a reduction in peak voltage drops shows an increase in voltage available at different nodes by up to 7 V in the worst case and 1.5 V on average. Optimization for total line losses shows a negligible savings across low voltage networks
Codes for replicating the results in the paper:
Naszodi, A., Cuccu, L., (2019). "Are high school degrees and university diplomas inherited in the U.S.? A new measure of relative intergenerational mobility"
Data for for Sperber, Clausen, Benke & Karnath - The anatomy of spatial neglect after posterior cerebral artery stroke
Included are all topographies and data underlying the main analyses and results of additional analyses. For more information see the README file. Use e.g. 7-zip freeware (https://www.7-zip.de/) to access the archived and compressed files.
The presented data article aims to provide the whole dataset obtained during an experiment of updating laser scan point clouds with photogrammetry meshes. In this context, the data quality and calculation time of photogrammetry models from different recording devices and different software solutions were compared. It was investigated whether photos from smartphones are also appropriate for updating point clouds by using photogrammetry in a factory environment. The photos of a technical installation were taken in 08:30 min with these three devices: Nikon D810 with Sigma art 24mm, iPhone 6 and iPhone XS. With each of the mentioned devices, three datasets have been created to provide enough data for the comparisons. One dataset (photos in .TIFF) of the iPhone XS is provided. The results of the data sets are used for a photogrammetry mesh quality comparison and a calculation time comparison. For the mesh quality comparison, visual qualitative inspections were performed on the models and the results were compared. Furthermore, all settings in the RealityCapture BETA 126.96.36.19996 ppi and Meshroom 2019 2.0 software are provided. A comparison of the quality of the photogrammetric 3D meshes was performed by comparing the rendering results. The dataset of the iPhone XS can be used to compare further photogrammetry software or single algorithms. Besides the images, the initial point cloud of the laser scanner is provided. Also included is the combined file which consists of the laser scan point cloud and the photogrammetry mesh of the end of the experiment.
This dataset contains three well-log data(D1,D2,X1),which located in Daguan area, northeast of Yunnan province, China. It is being made public both to act as supplementary data for publications of Qiao Wang and in order for other researchers to use this data in their own work. The purpose of the dataset was to investigate the shale gas resources in the Daguan area. All tests were performed on a well-Log equipment MTC6600, which tested the following parameters: GR, AC, SP, DEN, CNL, et al..This research project was made possible by a grant from the China Geological Survey Foundation.