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Primary and raw data from the manuscript "CACNA1S haploinsufficiency confers resistance to New World arenavirus infection"
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Identifying Factors Affecting E-customer Loyalty in Gamified Trusted Store Platforms: A Case Study Analysis in Iran
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Supplementary Data. Includes Excel data tables for ages and shapefiles for ages, geomorphology and ice-sheet reconstruction.
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The THA-col composite was prepared by mixing THA (2.5% w/v) and col 1 (0.5% w/v) with simultaneous gelation induced by hydrogen peroxide and horseradish peroxidase for THA and neutralization for col. The composite biomaterial ink was characterized for fibrillation by turbidity measurement, fluorescence microscopy and rheological properties (shear thinning behaviour and amplitude sweep) were assessed. Anisotropic properties were introduced upon 3D bioprinting (3D DiscoveryTM, RegenHU) the biomaterial ink. Cellular behaviour was investigated by embedding human mesenchymal stromal cell (MSC) spheroids into the hydrogel. Cell migration as well as chondrogenic differentiations was analysed over time by microscopy with subsequent image analysis (Oval plugin, ImageJ, NIH), gene expression analysis, histological stainings and glycosaminoglycan and DNA quantification. The fibrillation of col 1 and the homogenous distribution of the fibers within THA was shown by second harmonic generation imaging and fluorescent imaging and confirmed by turbidity measurement. After 3D bioprinting an anisotropic alignment of col fibrils was achieved that guided cell migration along the fiber orientation. Cell migration of hMSC spheroids showed similar behavior comparing THA-col and col whereas no migration was present for THA only. Chondrogenic differentiation resulted in an increase in cartilage related genes (col 2, aggrecan) with low tendency of hypertrophy (col X, col I, RunX2). Cartilage like matrix deposition was further corroborated by quantification of GAGs within samples that showed an overall increase within 21 days of culture similar to hMSC pellet control group. Safranin O staining resulted confirmed production of proteoglycans . Extrusion based printing has been investigated to produce scaffolds with anisotropic properties on microscale exploiting the shear forces inducing alignment of col fibres within a shear thinning HA matrix. The combination of the two matrix components brings unique features and advantages addressing cell migration, differentiation and material tissue integration compared to the single polymers. THA-col biomaterial has shown its potential for cartilage tissue engineering and represents a potential injectable material for cell free cartilage treatment.
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Supplementary data Calculated values for Cobalt ferrites (CoFe2O4) samples of pH = 7, 8 and 9. Lattice parameter (a), Crystallite size (D), Dislocation Density and micro strain, (dβcosθ)2 and d2βcosθ for Williamson-Hall plot, Average values of Lattice Parameter, Volume of unit cell V (e-30), Hopping length, Ionic Radii, Avogadro's number, x-ray density Δx (gr/cm3), Calculation of Lattice Parameter, Volume of unit cell V (e-30), Hopping length, Ionic Radii, Avogadro's number, x-ray density Δx (gr/cm3), Texture Coefficient, Tc, Standard Deviation, Stalking fault coefficient, α, I/Io and Tc, LA and LB
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Data Set for the experimenter 1 (Critical N application rates for tomato); Table S1: Tissue nutrient concentration of 5th leaf at vegetative stag (3 WAP) of tomato, Table S2: Shoot dry weight and tissue nutrient concentration at applied N rates in early reproductive stage (7WAP), Table S3: Tissue nutrient concentration at applied N rates in middle reproductive stage of tomato, Table S4: Tissue nutrient concentration of at applied N rates in late reproductive stage of tomato. Data Set for the experimenter 2 (Effect of N application rate at the vegetative growth stage on the marketable yield of tomato); Table S5: Applied N-rates for each treatment at different stages of tomato in Exp.2, Table S6: Comparison of yield data of two different N-treatments of tomato.
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Laboratory abnormalities in patients with livedoid vasculopathy
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This is the Supplementary Information file for: "Shifting modes of iron sulfidization at the onset of OAE-2 drive regional shifts in pyrite δ34S records". This includes Figures A1 to A6, sample ID information (Table A1), all SIMS data (Table A2), results of statistical tests applied to intra-grain SIMS data (Table A3), a written explanation of statistical tests, and two additional references.
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Microcracks accumulate around the inner ear. These data represent intersections of microcracks in the human otic capsule with unbiased stereological line grids. The allow the calculation of the surface density (mm2/mm3) of microcracks.
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