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The aim of this research is to find how humen react to vibrational excitations in road field test environment.
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Exome Array Data from 1019 Han Chinese Bladder Cancer Cases
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The THA-col composite was prepared by mixing THA (2.5% w/v) and col 1 (0.5% w/v) with simultaneous gelation induced by hydrogen peroxide and horseradish peroxidase for THA and neutralization for col. The composite biomaterial ink was characterized for fibrillation by turbidity measurement, fluorescence microscopy and rheological properties (shear thinning behaviour and amplitude sweep) were assessed. Anisotropic properties were introduced upon 3D bioprinting (3D DiscoveryTM, RegenHU) the biomaterial ink. Cellular behaviour was investigated by embedding human mesenchymal stromal cell (MSC) spheroids into the hydrogel. Cell migration as well as chondrogenic differentiations was analysed over time by microscopy with subsequent image analysis (Oval plugin, ImageJ, NIH), gene expression analysis, histological stainings and glycosaminoglycan and DNA quantification. The fibrillation of col 1 and the homogenous distribution of the fibers within THA was shown by second harmonic generation imaging and fluorescent imaging and confirmed by turbidity measurement. After 3D bioprinting an anisotropic alignment of col fibrils was achieved that guided cell migration along the fiber orientation. Cell migration of hMSC spheroids showed similar behavior comparing THA-col and col whereas no migration was present for THA only. Chondrogenic differentiation resulted in an increase in cartilage related genes (col 2, aggrecan) with low tendency of hypertrophy (col X, col I, RunX2). Cartilage like matrix deposition was further corroborated by quantification of GAGs within samples that showed an overall increase within 21 days of culture similar to hMSC pellet control group. Safranin O staining resulted confirmed production of proteoglycans . Extrusion based printing has been investigated to produce scaffolds with anisotropic properties on microscale exploiting the shear forces inducing alignment of col fibres within a shear thinning HA matrix. The combination of the two matrix components brings unique features and advantages addressing cell migration, differentiation and material tissue integration compared to the single polymers. THA-col biomaterial has shown its potential for cartilage tissue engineering and represents a potential injectable material for cell free cartilage treatment.
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Time series single cell RNA sequencing analysis of smooth muscle cell (SMC) to mesenchymal cell transition process in TGFβR2iSMC-Apoe-/- and Apoe-/- mice. Chromatin immunoprecipitation DNA sequencing was done to identify the potential binding sites of DNA-associated proteins in human aortic smooth muscle cells treated with TGFb or PBS.
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Characterization of TGFβR2iSMC-Apoe-/- mice after 1 month of high cholesterol high fat by histology, imaging mass cytometry (IMC), histoCAT, CyTOF, and immunocytochemistry. Aortic smooth muscle cells from TGFβR2iSMC-Apoe-/- express stem cell markers (CD105, CD73, CD90, Sca-1, CD44), mesenchymal cell markers (Osteopontin, Aggrecan, Adiponectin). To show clonal origin of smooth muscle cell-derived mesenchymal cells in TGFβR2iSMC-Apoe mice-/-, we replaced mTmG reporter strain TGFβR2iSMC-Apoe mice generating Myh11CreERT2;Tgfbr2f/f;Apoe-/-;Confettif/f and Myh11CreERT2;Apoe-/-:Confettif/f (control) lines.
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Data Related to Fig 2. A mouse line carrying floxed Tgfbr2 and SMC lineage-tracing mT/mG alleles under control of a myosin heavy chain (Myh11) promoter on an Ldlr null background (Ldlr-/-;Myh11CreERT2;mT/mGf/f;Tgfbr2f/f) hereafter called TGFβR2iSMC-Ldlr. These mice were treated with Tamoxifen at 6 week of age and fed high cholesterol high fat diet for 4 months. Ascending aorta smooth muscle cells from these mice express macrophage, chondrocyte, adipocyte, and osteoblast lineage markers as shown by immunocytochemistry and qRT-PCT from laser micro dissection tissues compared to controls.
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In the current data article, we present detailed characteristics of voids in carbon/epoxy composite laminates as well as the original image stacks, obtained via X-ray micro-Computed Tomography (micro-CT) . Five different lay-ups are produced with altering the recommended cure cycle in order to intentionally induce voids in the material. For each lay-up, an image stack (consisting of tomographic slices) and a dataset are provided. The image slices are in 8-bit TIF format. The datasets (spreadsheets) include the volume, size parameters, shape parameters, orientation, and location of all the detected voids in the specimen. The segmentation of the images and quantification of voids are performed in VoxTex, an in-house software for processing of micro-CT results. The data is linked to a Data in Brief article "A dataset of voids’ characteristics in multidirectional carbon fiber/epoxy composite laminates, obtained using X-ray micro-computed tomography" and linked to the article "Mehdikhani et al. Detailed characterization of voids in multidirectional carbon fiber/epoxy composite laminates using X-ray micro-computed tomography. Comp Part A. in press.".
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Raw data for experimentation of photosensitivity of fungal substrates
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Smooth muscle cell (SMC) TGFb signaling and expression of relevant genes in the media of normal human aortas and non-Marfan atherosclerotic ascending aorta aneurysms by bulk RNAseq and immunocytochemistry. Related To Fig 1. SMC aneurysms samples show a reduction in TGFb signaling and increased expression of inflammation-related genes compared to normal aortas SMCs.
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