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  • The image is an enhanced image sample of ancient ceramics. M_ming means plum vase in the Ming dynasty. M_qing means plum vase in the Qing dynasty. Y_ming means Jade pots of spring vase in the Ming dynasty. Y_qing means Jade pots of spring vase in the Qing dynasty.
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  • The THA-col composite was prepared by mixing THA (2.5% w/v) and col 1 (0.5% w/v) with simultaneous gelation induced by hydrogen peroxide and horseradish peroxidase for THA and neutralization for col. The composite biomaterial ink was characterized for fibrillation by turbidity measurement, fluorescence microscopy and rheological properties (shear thinning behaviour and amplitude sweep) were assessed. Anisotropic properties were introduced upon 3D bioprinting (3D DiscoveryTM, RegenHU) the biomaterial ink. Cellular behaviour was investigated by embedding human mesenchymal stromal cell (MSC) spheroids into the hydrogel. Cell migration as well as chondrogenic differentiations was analysed over time by microscopy with subsequent image analysis (Oval plugin, ImageJ, NIH), gene expression analysis, histological stainings and glycosaminoglycan and DNA quantification. The fibrillation of col 1 and the homogenous distribution of the fibers within THA was shown by second harmonic generation imaging and fluorescent imaging and confirmed by turbidity measurement. After 3D bioprinting an anisotropic alignment of col fibrils was achieved that guided cell migration along the fiber orientation. Cell migration of hMSC spheroids showed similar behavior comparing THA-col and col whereas no migration was present for THA only. Chondrogenic differentiation resulted in an increase in cartilage related genes (col 2, aggrecan) with low tendency of hypertrophy (col X, col I, RunX2). Cartilage like matrix deposition was further corroborated by quantification of GAGs within samples that showed an overall increase within 21 days of culture similar to hMSC pellet control group. Safranin O staining resulted confirmed production of proteoglycans . Extrusion based printing has been investigated to produce scaffolds with anisotropic properties on microscale exploiting the shear forces inducing alignment of col fibres within a shear thinning HA matrix. The combination of the two matrix components brings unique features and advantages addressing cell migration, differentiation and material tissue integration compared to the single polymers. THA-col biomaterial has shown its potential for cartilage tissue engineering and represents a potential injectable material for cell free cartilage treatment.
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  • Study aim: To study Gamma-enhancing neurofeedback learning process and evaluate its efficacy on visual feature binding and fluid intelligence Sample size: 18 healthy female students (mean age: 24.24 ± 1.94 years) Dataset: ----------- 1- Demographics: 18 subjects, Age, BMI, Weight, Height, Handedness, GPA 2- IQ measure: 18 subjects, Pretest and posttest sessions 3- Visual feature binding measure: 18 subjects, Pretest and posttest sessions, Response time and Error rate 4- 4 activity baseline EEG: 18 subjects, Pretest and posttest sessions, Tasks: Eyes open, Eyes closed, Auditory sensory attentiveness, Cognitive effort 5- Neurofeedback training EEG: 8 subjects, 8 training sessions, Eyes closed baseline EEG recorded before and after training in each session, EEG recorded during training in each session
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  • Distinguishing between bull Y- and X-bearing sperm populations is advantageous for techniques with sexed bull semen. The aim of this study was to produce a single-chain fragment variable (scFv) antibody against plasma membrane epitopes on bull Y-bearing sperm to distinguish between Y- and X- sperm. Variable heavy (VH) and variable light (VL) region genes generated from a hybridoma cell secreting a specific Y-bearing sperm monoclonal antibody (mAb-1F9) were cloned and expressed. The expected sizes of the DNA bands were ~350 bp for the VH gene and ~318 bp for the VL gene. The VH and VL genes were generated and used to construct an scFv gene (~650 bp) and express the corresponding soluble scFv antibody. Compared with the parent mAb-1F9, the scFv antibodies presented a high affinity for Y-bearing sperm and low cross-reactivity with X-bearing sperm. An immunofluorescence analysis confirmed that the scFv antibodies and mAb-1F9 recognize epitopes on the Y-bearing sperm surface. The fluorescence signal was strong on the plasma membrane of Y-bearing sperm but very weak for X-bearing sperm. This study helps the application and production of engineered scFv antibodies specific to Y-bearing sperm to distinguish between Y- and X-bearing sperm populations for techniques involving sexed bull semen
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  • Hardware design for build a Step Width System Capture
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  • Raw test data from SEAI funded ACCORD project. Results are from helical foil fatigue testing under radial load at ultimate limit state for ORPC's RivGen Power System. Load was applied at two locations along foil span to test turbine foil strength as well as composite clamp joint strength. The data highlights that the joint design is capable of handling the load applied where strain increase with cycles of load converged over time.
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  • Ab initio calculation files (Gaussian output files, video files, python code files). If any of these data is helpful, please cite the original paper: https://doi.org/10.1002/rcm.8790
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  • MicroCT stack of slice images of Tanaid crab that lives in small shell. Data set slice resolution (isotropic voxel size 2.028815 micrometers). MicroCT scanned at Stellenbosch CT facility using Nanotom S system, VGSTUDIO MAX 3.2 used for processing the data (de-noising).
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  • This article is investigating features of the microflora formation in newborns depending on the mother microbiological profile and locality altitude. Studying the problem of physiological and social adaptation of a person in extreme conditions of mountains and ensuring vital functions, in particular of microflora in unusual conditions is relevant and of significant priority. As it is known, the species composition of human microflora is constantly changing, and its stability is relative. This work is aimed to study the quantitative features and types of microbial colonization in newborns, depending on the microbiological profile of the mother in mountain conditions.
    Data Types:
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